Cytotoxic Antibody Response to Tumors Induced in Adult and Newborn Rabbits by Fibroma Virus

Abstract

was obtained from Harry Hinze, Univ. of Wisconsin, Madison, Wisconsin. DRK3 cells were grown in medium 199 supplemented with 10% fetal calf serum (FCS), 100 units of penicillin/ml, 100 1Ag of streptomycin/ml, and 0.075% sodium bicarbonate. PRK cells were grown in Eagle medium supplemented with 10% FCS, antibiotics, and sodium bicarbonate. Primary rabbit skin fibroblasts (RSF) were prepared from 4week-old rabbits. The rabbits were bled by cardiac puncture and the backs were shaved and washed with 70% alcohol. An excision was made down the back, the skin was reflected, and a piece was excised and placed in a petri dish. The hypodermis layer was re-on August 1, 2020 by guest http://iai.asm.org/ Downloaded from INFECT. IMMUNITY on August 1, 2020 by guest http://iai.asm.org/ Downloaded from

Depressed Cell-Mediated Immunity in Newborn Rabbits Bearing Fibroma Virus-Induced Tumors

“…The tumors increased in size during this time despite the increasing antibody titer. These results are in agreement with those of Allison (1) and with our previous demonstration of cytotoxic antibodies in newborn rabbits with progressively growing tumors (7). Delayed hypersensitivity reaction in adult and newborn tumor-bearing rabbits.…”

“…of tumor growth and regression, as described previously (7). In contrast, eight newborn tumorbearing rabbits showed no delayed hypersensitivity reaction to fibroma antigens when tested at weekly intervals for 4 weeks after tumor induction.…”

“…The delayed hypersensitivity reaction was readily demonstrable in adult tumor-bearing rabbits by the 1st week after 6,17 VO0L. 7,1973 on September 17, 2020 by guest http://iai.asm.org/ Downloaded from tumor induction and at all times tested thereafter. This has to be interpreted with some caution, since, as stated by Allison (1), it is technically difficult to introduce antigen into the thin skin of newborn rabbits anid be certain the antigein is retained at the site of inoculation.…”

“…Circulating antiviral antibody has been demonstrated by 7 days after virus inoculation of adult rabbits (1). Similarly, antibody cytotoxic for fibromainfected cells appears by the end of the first week of tumor growth and increases in titer as the tumors regress (5,7).…”

In Vitro Cellular Immunity to Unrelated Pathogens in Chickens Infected with Fowlpox Virus

“…Primary rabbit kidney cells were prepared by trypsinization of kidneys from 3-week-old rabbits and grown in Eagle medium supplemented as above with the exception of tryptose phosphate broth. A cloned line of New Zealand white rabbit kidney cells (DRK3) were grown as described (21).…”