Cytotoxic, antioxidant and antimicrobial properties of red sweet pepper (Capsicum annuum L. var. Llanerón) extracts: In vitro study

Abstract

Alcoholic and aqueous extracts were obtained from red sweet pepper (Capsicum annuum L.) by different methodologies to evaluate their cytotoxic, antioxidant and antimicrobial properties. Alcoholic extracts (MFP, MSd, SFP, SDP, SSd) from fresh red sweet pepper (FP) and dry pulp (DP) and seed (Sd) were obtained by maceration (M) and Soxhlet (S) equipment using methanol as extraction solvent; whereas aqueous extracts (LFP, LSd) were obtained by decoction followed by lyophilization (L). Human tumoral cell lines from breast (MCF-7 and SKBr3), prostate (PC3) and cervix (HeLa), and fibroblasts (as control) were used to determine the cytotoxic properties by the MTT assay. Antioxidant and antimicrobial properties were determined by DPPH and disc diffusion method, respectively. The extracts SDP and SFP showed the higher cytotoxic activity. The SDP extract had a significant (P < 0.05) in-vitro effect on HeLa (1.9 ± 1.4 µg/mL) and PC3 (< 1 µg/mL) cells with a moderated impact on fibroblasts (26.1 ± 1.2 µg/mL); whereas, SFP had a significant (p < 0.05) effect on MCF-7 cell line (2.1 ± 1.2 µg/mL) with a moderated impact on fibroblasts (25.9 ± 1.0 µg/mL). The higher antioxidant activity was found for MFP (80.3 ± 0.2%) and SFP extracts (75.5 ± 0.5%). Mild antimicrobial activity was only observed for alcoholic extracts. The results showed the potential of red sweet pepper (C. annuum L.) as a source of antioxidant and cytotoxic compounds, and suggest the need of further studies to isolate and characterize the bioactive compounds that impart those properties.

Preparation and Evaluation of Gallate Ester Derivatives Used as Promising Antioxidant and Antibacterial Inhibitors

“…The antibacterial rate was determined by the spread‐plate method [54] . A mixture of gallate ester derivative (1.00 mL, 300 μg mL −1 ) and bacterial suspension (15.00 mL) was placed in a glass tube and cultivated at 37 °C for 18 h. Then, the dilutions (0.20 mL) were spread evenly on nutrient agar, the colonies were counted after 18 h with three parallel samples for every concentration [55] . The antibacterial rate was calculated based on equation 2. …”

Pharmacological Properties and Health Benefits of Capsicum Species: A Comprehensive Review

“…These illnesses can generate many ailments, ranging from stomach discomfort to spontaneous abortions in pregnant women, and can even lead to death in severe cases. Researchers have recently stated that some varieties of chili peppers and their active compounds exhibit significant antimicrobial properties, equivalent to some modern-day antibiotics [73][74][75][76]. Especially, Goci et al investigated the carbopol-based formulated capsaicin enhances the antibacterial and antifungal effects against Escherichia coli (ATCC 10536), Bacillus cereus (Peru MycA 4), Salmonella typhi (Peru Myc 7), Staphylococcus aureus (ATCC 6538), Candida tropicalis (YEPGA 6184), Candida albicans (YEPGA 6379), Candida parapsilosis (YEPG 6551), and Candida albicans (YEPGA 6183), with a minimum inhibitory concentration (MIC) value reduction of at least 50% [77].…”

Effect of Elicitors as Stimulating Substances on Sensory Quality Traits in Color Sweet Bell Pepper (Capsicum annuum L. cv. Fascinato and Orangela) Grown under Greenhouse Conditions

“…Sweet pepper (Capsicum annuum, L.) is one of the most important commercial vegetable crops worldwide and a component of the traditional human diet, it is a good source of antioxidants, fl avonoids, phenolic acids and carotenoids [Castro et al, 2008;Ilić et al, 2017;Raybaudi-Massilia et al, 2017]. Consumers purchasing decisions are commonly focused on the color, size, fi rmness and other quality features [Maalekuu et al, 2004].…”