Cytotoxic cytochalasins from the endozoic fungus Phoma sp. of the giant jellyfish Nemopilema nomurai

Kim, Eun La; Li, Jian Lin; Dang, Hung; Hong, Jongki; Lee, Chong-Ok; Kim, Dong‐Kyoo; Yoon, Won Duk; Kim, Euikyung; Liu, Yonghong; Jung, Jee H.

doi:10.1016/j.bmcl.2012.03.058

Cited by 41 publications

(29 citation statements)

References 31 publications

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“…To reconstruct the phylogenetic tree, another five relative sequences from four identified jellyfish-derived fungi, Epicoccum purpurascens [ 19 ], Phoma sp. [ 47 ], Aspergillus fumigatus [ 48 ] and Paecilomyces variotii [ 17 ], were used. These five ITS sequences were FR822845, GQ352490 [ 18 ], KH00184 [ 18 ], JX022941 and JN8509969, respectively.…”

Section: Methodsmentioning

confidence: 99%

Exploring the Antibacterial and Antifungal Potential of Jellyfish-Associated Marine Fungi by Cultivation-Dependent Approaches

Yue

et al. 2015

PLoS ONE

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Fungi isolated from marine invertebrates are of considerable importance as new promising sources of unique secondary metabolites with significant biomedical potential. However, the cultivable fungal community harbored in jellyfish was less investigated. In this work, we seek to recover symbiotic fungi from different tissues of jellyfish Nemopilema nomurai. A total of seven morphotypes were isolated, which were assigned into four genera (Aspergillus, Cladosporium, Purpureocillium, and Tilletiopsis) from two phyla (Ascomycota and Basidiomycota) by comparing the rDNA-ITS sequences with the reference sequences in GenBank. The most fungi were found in the inner tissues of subumbrella. Two of the cultivation-independent procedures, changing media type and co-cultivation, were employed to maximize the complexity of metabolites. Thus, thirteen EtOAc gum were obtained and fingerprinted by High Performance Liquid Chromatography (HPLC) equipped with a photodiode array (PDA) detector. Antibacterial and antifungal activities of these complex mixtures were tested against a panel of bacterial and fungal pathogens. The antimicrobial results showed that all of the 13 EtOAc extracts displayed different levels of antibacterial activity, three of which exhibited strong to significant antibacterial activity to the bacterial pathogens Staphylococcus aureus and Salmonella entrica. Antifungal activity indicated that the EtOAc extracts from pure culture of Aspergillus versicolor and co-culture of A. versicolor and Tilletiopsis sp. in rice media were promising for searching new compounds, with the maximal mycelial growth inhibition of 82.32% ± 0.61% for Rhizoctonia solani and 48.41% ± 11.02% for Botrytis cinerea at 200 μg/ml, respectively. This study is the first report on the antibacterial and antifungal activity of jellyfish-associated fungi and allows the first sight into cultivable fungal community residing in jellyfish. Induced metabolites by cultivation-dependent approaches provides a new reservoir for drug discovery from jellyfish-derived fungi.

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Section: Methodsmentioning

confidence: 99%

Exploring the Antibacterial and Antifungal Potential of Jellyfish-Associated Marine Fungi by Cultivation-Dependent Approaches

Yue

et al. 2015

PLoS ONE

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“…1) was isolated from Phoma sp. fungus obtained from the giant jellyfish Nemopilema nomurai (22). This toxin interferes with cytoskeleton functions by inhibiting actin polymerization (23,24).…”

Section: Introductionmentioning

confidence: 99%

Cytochalasin B induces apoptosis through the mitochondrial apoptotic pathway in HeLa human cervical carcinoma cells

Hwang

Zhang

et al. 2013

Oncology Reports

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Cytochalasin B (CB) is a cell-permeable mycotoxin. It inhibits cytoplasmic division by blocking the formation of contractile microfilaments, it inhibits cell movement and induces nuclear extrusion. In the present study, we investigated the anticancer activity of CB in HeLa human cervical carcinoma cells. CB showed significant cytotoxicity, with an IC50 of 7.9 µM, in a WST-8 assay and significantly inhibited cell proliferation. Furthermore, results from Annexin V-FITC/propidium iodide double-staining indicated that CB induced early apoptosis of HeLa cells in a time-dependent manner. The cells exhibited apoptotic morphology, including cell shrinkage and nuclear condensation. CB induced cell cycle arrest at the S phase. We also observed inhibition of DNA replication in a [3H]-thymidine incorporation assay. Furthermore, CB induced a time-dependent increase in reactive oxygen species and a decrease in mitochondrial membrane potential. Western blot analysis showed an increase in levels of mitochondrial factors Bax and Bcl-2, which was followed by activation of caspase-9 and -3. These results suggested that CB induced apoptosis via a mitochondrial-dependent pathway in HeLa cells.

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“…The number of fungal isolates received from twigs, shells and leaves of Corylus avellana was greater than those obtained from young and mature leaves of Ocimum basilicum. The endophytic fungal isolates were identified and belonged to 14 commonly isolated endophytic genera such as Alternaria, Fusarium, Phoma, Pestalotiopsis, which have also been reported as producers of the anticancer compounds: taxol, cytochalasins, podophyllotoxin [25][26][27][28][29]. Among all the isolates, Phoma with about 19% isolates was the dominant genus.…”

Section: Resultsmentioning

confidence: 99%

Plant endophytic fungi as a source of paclitaxel

Michalczyk

Cieniecka‐Rosłonkiewicz

Cholewińska

2015

Herba Polonica

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Summary Endophytic fungi were isolated from different parts of the plants Corylus avellana (Corylaceae) and Ocimum basilicum (Lamiaceae) and then identified to the genus level based on the morphology of the fungal culture and the characteristics of the spores. The fungicidal and antitumor activity of isolates and extracts obtained from them was determined. We found that a few isolates from Corylus avellana and Ocimum basilicum produced metabolites that inhibited the growth of Oomycetes fungi to a highly significant extent. In the potato disc bioassay only an extract from the isolate C-9 showed an inhibitory action in tumor development. Paclitaxel presence in extract from the isolate C-9 was confirmed using the thin layer chromatography method (TLC) and UV-VIS spectroscopic analysis. The above results indicated that the tested samples revealed an antitumor activity. The detection of paclitaxel in the isolate C-9 suggests that the antitumor activity resulted probably from the presence of this taxane in the tested material.

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Cytotoxic cytochalasins from the endozoic fungus Phoma sp. of the giant jellyfish Nemopilema nomurai

Cited by 41 publications

References 31 publications

Exploring the Antibacterial and Antifungal Potential of Jellyfish-Associated Marine Fungi by Cultivation-Dependent Approaches

Exploring the Antibacterial and Antifungal Potential of Jellyfish-Associated Marine Fungi by Cultivation-Dependent Approaches

Cytochalasin B induces apoptosis through the mitochondrial apoptotic pathway in HeLa human cervical carcinoma cells

Plant endophytic fungi as a source of paclitaxel

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