Cytotoxic Effect of Chitosan-H, Resveratrol, β-Carotene and Propolis and their Chitosan Hydrogels on Balb/C Mouse 3T3 Fibroblast Cells

Abstract

Chitosan [10] which is produced commercially by de-acetylation of chitin is a natural polysaccharide composed of randomly distributed β-1, 4-linked D-glucosamine and N-acetyl α-glucosamine.Chitosan is non-toxic, biocompatible, bio-degradable and has muco-adhesive properties and as a result became widely used in the pharmaceutical field as a carrier system for drugs, hormones, proteins, enzymes and genes [11][12][13][14]. Chitosan is positively charged in acid medium because the amino groups become protonated

Cytotoxicity of Different Degrees of De-Acetylated Chitosan on 3T3- and Two Human Tooth Fibroblast Cell-Lines

“…We determined the survival rates at a growth medium concentration of 1mg/ml which is reported to be in line with other research and which may be considered as on the upper recommended level [16] of concentration. The three cell lines tested consisted of a standard cell line bought from the National Repository for Biological Materials and two self-established human tooth pulp fibroblast cell lines (H1 cell line and H2 cell line) from two different people.…”

“…A Balb/c 3T3 mouse fibroblast cell line (The National Repository for Biological Materials, Sandringam) was maintained and cultured under standard conditions [13]. The two human dental pulp fibroblast cultures were established as previously described [14][15][16]. Briefly freshly extracted impacted 3 rd molars were collected in Dubecco's Modified Eagels Medium, the middle third of the pulp cultured and the cells between the 3 rd and 5 th passage used.…”

Towards Bioactive Dental Restorative Materials with Chitosan and Nanodiamonds: Evaluation and Application

“…In our previous studies [23] on cytotoxicity we found that the presence of chitosan with various substances, like anti-oxidants (resveratrol, propolis and β-carotene) and others (naproxen, ibuprofen) increased the cell survival rate of mouse 3T3 fibroblast cells significantly in comparison to the mentioned substances alone.…”

“…The cytotoxicity was examined as previously reported [23]. Briefly, the cells were first grown to near confluency, diluted to a final cell suspension containing approximately 3×10 5 cells/ml and plated out insets of 96 well plates.…”

Chitosan Bio-Active Designer Materials and Orthodontics: Development and Evaluation of Novel Materials as Enamel Protective Agents

“…A chemical bonding capacity through the interaction between some functional monomers and the calcium of residual hydroxyapatite may contribute favorably to the bonding effectiveness [26][27][28][29] but, fluoride affects the enamel surface rendering it more resistant to demineralization. Fluoride in low concentrations favors the formation of fluoro-hydroxyapatite, which is less susceptible to acidic solubility than hydroxyapatite [34,35]. Therefore, it is recommended to use these preventive agents after bonding the brackets when self-etch adhesive systems are used.…”