Cytotoxic effect of<i>Moringa oleifera</i>on colon cancer cell lines

Reda, Farah; Borjac, Jamilah; Fakhouri, R.; Usta, Jinan

doi:10.17660/actahortic.2017.1158.30

Cited by 13 publications

(5 citation statements)

References 20 publications

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“…Antiproliferative effect of its methanol extract on HCT‐116 human colorectal carcinoma cells showed a cytotoxic effect due to components of such as astragalin and isoquercetin (Tragulpakseerojn et al, ). Aqueous extracts showed cytotoxicity on colon cancer cell lines HTC116, HCT116P53 and Caco2 (Reda, Borjac, Fakhouri, & Usta, ).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant traits and protective impact ofMoringa oleiferaleaf extract against diclofenac sodium-induced liver toxicity in rats

El-Hadary

Ramadan

2018

J Food Biochem

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Moringa oleifera gained importance as a medicinal plant. The current study assesses Moringa leaf ethanol extracts (MLE) against experimentally diclofenac sodium (DcNa)‐induced liver toxicity in male rats. Leaves were extracted with different solvents differing in polarity. Assessment involved total phenolic compounds, total flavonoids and radical scavenging activity against 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH·). HPLC was performed for identifying phenolic compounds, wherein ethyl vanillin (1,205 mg/kg), 3‐OH‐tyrosol (812.2 mg/kg), benzoic acid (273.8 mg/kg), salicylic acid (240.0 mg/kg), chlorogenic acid (233.3 mg/kg) and 3,4,5‐methoxy‐cinnamic acid (172.5 mg/kg) were measured. Fifty animals (each treatment group consisted of 10 rats) were subjected to five treatments and the experiment lasted for 4 weeks. Animals were exposed to DcNa (100 mg/kg) and two doses of MLE as well as silymarin (an antioxidant flavonoid C25H22O10) for 4 weeks. Liver marker enzymes, including alkaline phosphatase, alanine transaminase, and aspartate transaminase as well as urea, uric acid, and creatinine were increased. Serum albumin and total protein decreased in DcNa‐treated rats. Homogenates nitric oxide increased in liver tissue of the DcNa‐treated rats, while the activity of each of glutathione peroxidase, glutathione‐S‐transferase, glutathione, and catalase decreased. It could be concluded that MLE in both doses and silymarin are considerably hepatoprotective with antioxidant activity (AOA) against DcNa‐induced hepatotoxicity in rats. Practical applications Administration of MLE caused improvements in kidney functions and acted as antioxidant enzymes as compared with silymarin (as a reference drug). AOA was exhibited by MLE in vivo, and this would have a positive effect against oxidative liver damage caused by DcNa. Plasma membrane was protected and the regenerative and reparative capacity of liver increased by phenolics in the MLE. The study demonstrated the MLE hepatoprotective activity and recommends using M. oleifera leaves for the treatment of liver disorders.

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Section: Introductionmentioning

confidence: 99%

Antioxidant traits and protective impact ofMoringa oleiferaleaf extract against diclofenac sodium-induced liver toxicity in rats

El-Hadary

Ramadan

2018

J Food Biochem

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“…A glucosinolate-rich hydrolyzed extract from Moringa oleifera only showed significant effects on LDH release for HT-29 but not for HCT116 cells, compared to their untreated counterparts [ 16 ]. Other authors evaluating the impact of Moringa oleifera also found increased LDH release in HCT116, Caco-2, and HCT116 p53 −/− cells, and the activity was presumably linked to the isothiocyanate content of the leaves [ 61 ]. Since most isothiocyanates are released from vegetal sources due to bacterial enzymatic activity, it could be inferred that the colonic microbiota activity could release these compounds to exert their necrotic effect.…”

Section: Discussionmentioning

confidence: 99%

Isothiocyanate-Rich Extracts from Cauliflower (Brassica oleracea Var. Botrytis) and Radish (Raphanus sativus) Inhibited Metabolic Activity and Induced ROS in Selected Human HCT116 and HT-29 Colorectal Cancer Cells

Cuellar-Núñez

Luzardo-Ocampo

Lee-Martínez

et al. 2022

IJERPH

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Cruciferous vegetables such as cauliflower and radish contain isothiocyanates exhibiting chemoprotective effects in vitro and in vivo. This research aimed to assess the impact of cauliflower (CIE) and radish (RIE) isothiocyanate extracts on the metabolic activity, intracellular reactive oxygen species (ROS), and LDH production of selected human colorectal adenocarcinoma cells (HCT116 and HT-29 for early and late colon cancer development, respectively). Non-cancerous colon cells (CCD-33Co) were used as a cytotoxicity control. The CIE samples displayed the highest allyl isothiocyanate (AITC: 12.55 µg/g) contents, whereas RIE was the most abundant in benzyl isothiocyanate (BITC: 15.35 µg/g). Both extracts effectively inhibited HCT116 and HT-29 metabolic activity, but the CIE impact was higher than that of RIE on HCT116 (IC50: 0.56 mg/mL). Assays using the half-inhibitory concentrations (IC50) of all treatments, including AITC and BITC, displayed increased (p < 0.05) LDH (absorbance: 0.25–0.40 nm) and ROS release (1190–1697 relative fluorescence units) in both cell lines. BITC showed the highest in silico binding affinity with all the tested colorectal cancer molecular markers (NF-kB, β-catenin, and NRF2-NFE2). The theoretical evaluation of AITC and BITC bioavailability showed high values for both compounds. The results indicate that CIE and RIE extracts display chemopreventive effects in vitro, but additional experiments are needed to validate their effects.

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“…Thus, suggesting that this plant might be significantly toxic to the cells (9). Reda et al (2017), demonstrated that Moringa oleifera leaves water extracts have shown to reduce the viability of colon cells (HCT116, CACO2, and HCT116P53-/-) in a concentration-dependent manner varying from 0.1 to 2.5 per cent, via allocation of membrane integrity and cell cycle arrest in subG0 phase. Increased in oxidative stress and stimulate ROS producing and lead to LDH (is a cytosolic enzyme led to oxidizes l-lactate to pyruvate) emission.…”

Section: Induction Of Apoptosis On Human Colon Cancer Cell Line (Hct ...mentioning

confidence: 99%

“…Increased in oxidative stress and stimulate ROS producing and lead to LDH (is a cytosolic enzyme led to oxidizes l-lactate to pyruvate) emission. The release from the cytoplasm to the medium, therefore, displayed changes in the permeability of the plasma membrane elicit the incidence of necrosis or apoptosis (10).…”

Section: Induction Of Apoptosis On Human Colon Cancer Cell Line (Hct ...mentioning

confidence: 99%

“…A study by Reda et al (2017), demonstrated that phytochemicals, for example glucosinolates (GLs) and their breakdown products (i.e., ITCs as benzyl ITC, allyl ITC, phenethyl ITC, and sulforaphane) were subjected to avert the risk of carcinogenesis (10). ITCs are a category of natural products which produced and released myrosinase enzymatic action on their GLs precursors after cell damage (26).…”

Section: Cell Cycle Analysis On Moringa Oleifera Targeted Protein Pat...mentioning

confidence: 99%

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Molecular Targets of Moringa Oleifera in Cancers (In Vitro and in Vivo): A Systematic Review

Sharif Salhab,

Ziyad Mustafa,

Joseph

et al. 2023

JUMMEC

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Cancer is unquestionably the greatest worldwide threat to humanity. With fewer side effects, natural anticancer medicines are the most prevalent. Anticancer benefits of dietary polyphenols could be achieved through carcinogen elimination, cancer cell signalling alteration, antioxidant enzymatic activities, and apoptosis-inducing substances, according to the likely approach to anticancer effects of dietary polyphenols research. This study aims to investigate the apoptosis induction mediated by Moringa oleifera in in vitro and other molecular targets in vivo in all types of cancer studies, as well as to describe the most recent and comprehensive research on antioxidant and anticancer characteristics. The terms "molecular target", "signalling pathway", "apoptosis", "Moringa oleifera extract", "mechanism of action", "cancer", "tumour", "carcinoma", and "melanoma" were used to search four online databases, PubMed, Scopus, ScienceDirect, and Google Scholar, for relevant articles published between 2009 and 2019.

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Cytotoxic effect ofMoringa oleiferaon colon cancer cell lines

Cited by 13 publications

References 20 publications

Antioxidant traits and protective impact ofMoringa oleiferaleaf extract against diclofenac sodium-induced liver toxicity in rats

Antioxidant traits and protective impact ofMoringa oleiferaleaf extract against diclofenac sodium-induced liver toxicity in rats

Isothiocyanate-Rich Extracts from Cauliflower (Brassica oleracea Var. Botrytis) and Radish (Raphanus sativus) Inhibited Metabolic Activity and Induced ROS in Selected Human HCT116 and HT-29 Colorectal Cancer Cells

Molecular Targets of Moringa Oleifera in Cancers (In Vitro and in Vivo): A Systematic Review

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