Cytotoxic effect of jasmonate and methyl jasmonate on a canine macrophage tumor cell line

Abstract

HERNANDES, C.; CARDOZO, G.P.; FRANÇA, S.C.; FACHIN, A.L.; MARINS, M.*; LOURENÇO, M.V.Universidade de Ribeirão Preto, UNAERP, CEP: 14096-900, Ribeirão Preto-Brasil *marins@heranza.com.br ABSTRACT: Cancer is one of the leading causes of death in dogs, a fact that has boosted the investigation for new more specific antitumor drugs. This study evaluated the antitumor activity of jasmonates in the canine macrophage cell line DH82 (ATCC # CRL-10389) isolated from a case of malignant histiocytoma. The activities of methyl jasmonate and jasmonic acid were compared to that of doxorubicin by the MTT assay. Methyl jasmonate resulted in the highest inhibition of cell growth (82.2%), followed by doxorubicin (80.7%) and jasmonic acid (36.5%). More detailed studies regarding the action of jasmonates on animal health are necessary, but the present results indicate methyl jasmonate as an alternative to the development of drugs for the treatment of canine oncologies. Key words: jasmonates, histiocytoma, dogsRESUMO: Efeito citotóxico de jasmonato e metiljasmonato em linhagem tumoral celular de macrófagos caninos. O câncer é uma das principais causas de morte em cães o que tem impulsionado a pesquisa por novos antitumorais com ação mais específica. Neste trabalho, a atividade antitumoral de jasmonatos foi avaliada na linhagem de macrófagos caninos DH82 (ATCC # CRL-10389), a qual é originada de um caso de histiocitoma maligno. A atividade do metil jasmonato (MJ) e do ácido jasmônico (AJ) foram comparadas à doxorrubicina, através do método de coloração MTT. O MJ apresentou maior inibição (82,2%), seguido da Doxorrubicina (80,7%) e AJ (36,5%). Estudos mais completos da ação dos jasmonatos na saúde animal ainda são necessários, porém, os dados deste trabalho apontam o MJ como alternativa para o desenvolvimento de drogas para o tratamento das oncologias caninas.

Methyl Jasmonate: An Alternative for Improving the Quality and Health Properties of Fresh Fruits

“…Recently, Umukoro et al [118] found that MeJA exhibits specific anti-offensive aggressive activity, and they proposed this as a potential suitable treatment of reactive aggression in humans. Based on the promisingantitumor effects of JAs observed in animals, this molecule has been also proved as antitumor drugs for the treatment of canine oncologies [119,120]. Thus, there is evidences indicating that MeJA resulted in the highest inhibition of cell growth (82.2%), followed by doxorubicin (positive control, 80.7%) and JA (36.5%) of canine macrophage cell line DH82 (malignant histiocytoma) [120].…”

Interactions of Bacillus thuringiensis and organic biofertilizer as elicitors in Mikania laevigata and phytopathogenic action / Interações de Bacillus thuringiensis e biofertilizante orgânico como elicitores em Mikania laevigata e ação fitopatogênica

“…The use of AJ and MJ in stimulation experiments is a widely used strategy to increase the production of secondary metabolites in vegetables (PERASSOLO et al, 2011), acting as a stress signal, activating the plant defense mechanism that is fundamental for production of biomolecules of pharmacological interest (HERNANDES et al, 2012, MIERSCH et al, 1989.…”