Cytotoxic effect of Spirulina platensis extract and Ulva compressa Linn. on cancer cell lines

Abstract

Studies have shown that algae and seaweed have cytotoxic activity. This study was aimed to determine the cytotoxic activity of Spirulina platensis and Ulva compressa Linn. extracts against cancer cell lines. The cytotoxic activity of the extract was carried out using the MTT ((3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method. Results showed that the ethanol extract and methanol extract of Spirulina platensis have no cytotoxic effect against HeLa, WiDr, MCF7 and T47D cells. Water extract of Spirulina platensis had no cytotoxic activity on T47D and Vero cells. Water extracts of Spirulina platensis increase MCF7 cell growth. Phycocyanin powder also stimulates MCF7 cell growth. Ethanol extract of Ulva compressa Linn. exhibited potentially cytotoxic activity against MCF7 and moderate cytotoxic against WiDR cells with IC50 values are 31.86 μg/ mL and 104.93 μg/mL, respectively. It can be concluded that extract of Spirulina platensis has no potential to be developed for cancer therapy. Ulva compressa Linn has the potential to be developed as an anti-cancer. Further research for study the mechanism of anticancer of Ulva compressa Linn on MCF7 was needed.

Potential Activity of Arthrospira platensis as Antioxidant, Cytotoxic and Antifungal against Some Skin Diseases: Topical Cream Application

“…Cyanobacteria are rich in many bioactive compounds and have become a cheap and safe source of these compounds in many medicinal and pharmacological applications [ 18 ]. However, some variables, such as environment, season of collection and culture, growth stages and experimental procedures may impact cyanobacteria’s antifungal activity [ 19 ]. Although many studies have used several solvents to screen cyanobacteria for antimicrobial activity, it is still unknown which solvent is the most successful and acceptable for extracting algae for antifungal applications [ 10 ].…”

Baeckea frutescens L. Promotes wound healing by upregulating expression of TGF-β, IL-1 β, VEGF and MMP-2

“…Following treatment, cells were allowed to proliferate for an additional 24, 48, or 72 h before undergoing MTT assay. The MTT assay was conducted following a modified version of the protocol outlined by Maryati et al (2020) . The culture media were replaced with 100 μl of fresh media containing 0.5 μg/ml MTT and incubated for 4 h. Subsequently, the media were aspirated, and DMSO was added to solubilize the purple formazan crystals generated by viable cell mitochondria.…”

Penentuan Kadar Astaxanthin, Uji Antiinflamasi Dan Sitotoksik Terhadap Sel Kanker Payudara Ekstrak Aseton Spirulina Platensis

“…Spirulina platensis merupakan mikroalga yang telah diproduksi secara masal dan banyak dimanfaatkan sebagai suplemen karena mengandung zat yang memiliki manfaat bagi kesehatan, yaitu protein (60-63%), karbohidrat (16%), lemak (4%), dan berbagai macam mikronutrien seperti biopigmen berupa klorofil, karotenoid dan fikosianin [3][4] . Telah dilaporkan pada penelitian sebelumnya bahwa ekstrak etanol dan metanol Spirulina platensis memiliki efek sitotoksik terhadap sel kanker payudara MCF-7 dengan nilai IC50 masing-masing sebesar 114,014 mg/L dan 117,522 mg/L [5] .…”

“…Beberapa penelitian tentang uji sitotoksik ekstrak Spirulina platensis terhadap sel kanker payudara MCF-7 telah dilakukan sebelumnya, ekstrak etanol dan metanol Spirulina platensis dilaporkan memiliki aktivitas sitotoksik terhadap sel kanker payudara MCF-7 dengan nilai IC50 sebesar 114,014 mg/L dan 117,522 mg/L [5] . Berdasarkan nilai IC50, sitotoksik dari ekstrak aseton Spirulina platensis terhadap sel kanker payudara MCF-7 lebih tinggi dibandingkan ekstrak etanol dan metanol Spirulina platensis.…”