Cytotoxic effect of γ-sitosterol from Kejibeling (&lt;em&gt;Strobilanthes crispus&lt;/em&gt;) and its mechanism of action towards &lt;em&gt;c-myc&lt;/em&gt; gene expression and apoptotic pathway

Endrini, Susi; Rahmat, Asmah; Ismail, Patimah; Taufiq‐Yap, Yun Hin

doi:10.13181/mji.v23i4.1085

Cited by 35 publications

(26 citation statements)

References 12 publications

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“…Phytosterols, particularly γ-sitosterol and stigmasterol, are also important constituents of the extract (detected by GC-MS). γ-sitosterol was previously shown to be cytotoxic against colon and liver cancer cell lines by downregulating c-myc and inducing apoptosis [53]. Moreover, stigmasterol was formerly studied for activating apoptosis in hepatocellular carcinoma cell lines through upregulation of the Bax protein and downregulation of the Bcl-2 protein [54].…”

Section: Discussionmentioning

confidence: 99%

Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro

Khoury

Haykal

Hodroj

et al. 2020

Cancers

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Malva pseudolavatera Webb & Berthel. is a plant from the Malvaceae family that has long been included in the human diet due to its various curative effects. Many plant leaf extracts from the various species of Malva genus have been reported to possess anti-cancer properties, however, studies on M. pseudolavatera Webb & Berthel. leaves have documented anti-inflammatory and anti-oxidant effects with no emphasis on their possible anti-cancer potential. The present study explores the anti-cancer properties of Malva pseudolavatera Webb & Berthel. leaf extract on acute myeloid leukemia (AML) cell lines in vitro and deciphers the underlying molecular mechanism. Treatment of AML cell lines with M. pseudolavatera methanolic leaf extract showed a dose- and time-dependent inhibition of proliferation and a dose-dependent increase in apoptotic hallmarks such as an increase in phosphatidylserine on the outer membrane leaflet and membrane leakage in addition to DNA fragmentation. The pro-apoptotic effect was induced by reactive oxygen species (ROS) as well as an upregulation of cleaved poly(ADP-ribose) polymerase (PARP), increase in Bax/Bcl-2 ratio, andrelease of cytochrome-c from the mitochondria. Major compounds of the extract included methyl linolenate, phytol, γ-sitosterol, and stigmasterol as revealed by gas chromatography coupled with mass spectrometry, and amino acids, amino acid derivatives, tiliroside, 13-hydroxyperoxyoctadecadienoic, and quercitrin as detected by liquid chromatography coupled to mass spectrometry.

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Section: Discussionmentioning

confidence: 99%

Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro

Khoury

Haykal

Hodroj

et al. 2020

Cancers

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“…[19] In addition, γsitosterol from Strobilanthes crispus leaves extract was cytotoxic against colon and liver cancer cell lines. [20] Additionally, β-tocopherol and γ-tocopherol identified in this extract, are well known for their antioxidant properties. [21] Recent studies also have reviewed the benefits of these compounds such as anticancer, anti-inflammatory, and cancer preventive effects.…”

Section: Discussionmentioning

confidence: 92%

Phytochemical Screening and Cytotoxic Effects of Crude Extracts of Pereskia Bleo Leaves

Mohd-Salleh

Ismail

Wan-Ibrahim

et al. 2020

Journal of Herbs, Spices & Medicinal Plants

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The phytocompounds in crude solvent extracts of Pereskia bleo leaves were identified and their cytotoxic effects on cancer cell lines were determined. Crude extracts were obtained via maceration and subjected to GCMS analysis. Then, each extract was incubated with HeLa, MDA-MB-231, SW480, and NIH/3T3 cell lines for 72 h. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was done to determine IC 50 values of each extract. Terpenoids, sterols, alkaloids, fatty acids and phenolic compounds were identified from the crude extracts of P. bleo leaves. Other compounds identified were γ-sitosterol, β-tocopherol, and γ-tocopherol. The ethyl acetate extract had potent cytotoxic effect against HeLa and MDA-MB-231 cancer cells as noted by the lowest IC 50 values ARTICLE HISTORY

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“…The gamma sitosterol has already been described as cytotoxic against colon, liver, breast and lung human tumor cell lines [28,29]. The pharmacological mechanism was attributed to cell cycle arresting and apoptosis induction [29][30][31].…”

Section: Resultsmentioning

confidence: 99%

In vitro Antiproliferative Potential of Celtis iguanaea against Ovarian (OVCAR-3) and Colon (HT-29) Tumor Cell

Zanchet

Miorando

Gomes

et al. 2019

EJMP

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Natural products have been reported as a main source of anticancer molecules. The species Celtis iguanaea (Jacq.) Sarg., (Cannabaceae) is widely distributed in Brazil where it is known as “esporão-de-galo or taleira”. The leaves are popularly used as anti-inflammatory, in the treatment of body pain and urinary infections. However, the antiproliferative potential against human cancer cells remain to be elucidated. In this study, extracts and different fractions from the leaves of C. iguanaea were tested in vitro, against a panel tumor cell lines. The hydroalcoholic extract was inactive, while dichloromethane extract showed promisor antiproliferative effects. In turn, the dichloromethane fraction showed potent cytostatic activity against ovarian cell line (OVCAR-3, GI50 = 4.65 mg/ml). However, the stronger antiproliferative effects with high selectivity were observed for the hexane fraction that exhibited activity against ovarian (GI50 = 3.99 mg/ml) and colon (HT-29, GI50 = 3.16 mg/ml). The antiproliferative effects observed are probably related to the presence of 3,7,11,15-tetramethyl-2-hexadecen-1-ol and gamma-sitosterol present in the hexane fraction and detected by GC/MS. This is the first report of antiproliferative activity of C. iguanaea and the results suggested that the molecules of fraction hexane are promising chemotherapeutic compounds, especially against tumor cell of ovarian and colon.

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Cytotoxic effect of γ-sitosterol from Kejibeling (<em>Strobilanthes crispus</em>) and its mechanism of action towards <em>c-myc</em> gene expression and apoptotic pathway

Cited by 35 publications

References 12 publications

Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro

Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro

Phytochemical Screening and Cytotoxic Effects of Crude Extracts of Pereskia Bleo Leaves

In vitro Antiproliferative Potential of Celtis iguanaea against Ovarian (OVCAR-3) and Colon (HT-29) Tumor Cell

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