Cytotoxic Effects and Production of Cytokines Induced by the Endophytic Paenibacillus polymyxa RNC-D In Vitro

Neris, Débora Meira; Luna, Genoveva Lourdes Flores; Rodolpho, Joice Margareth de Almeida; Urbaczek, Ana Carolina; Correia, Ricardo de Oliveira; Feitosa, Karina Alves; Serrano, Nadja Fernanda Gonzaga; Sousa, Cristina Paiva de; Aníbal, Fernanda de Freitas

doi:10.2174/1389201018666171026155522

Cited by 4 publications

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“…Our results showed that the values of EC 50 obtained in the periods of 24 and 48 hours of leishmanicidal were 0.624 mg/mL and 0.547 mg/mL, respectively ( Table 1 ). Neris and collaborators [ 24 ] have previously observed the effect of the Paenibacillus polymyxa RNC-D extract in BALB/3T3 fibroblasts and J774A.1 macrophages. In the present study, it was reported that the mortality rate of 50% was around 1.171 mg/mL for fibroblasts and 0.994 mg/mL for macrophages in the period of 48 hours.…”

Section: Discussionmentioning

confidence: 99%

“…Our results showed that there was significant production of IL-12 in the infected group treated with 0.5 mg/mL ( Figure 2(d) ). For the other concentrations of the extract (0.1 and 1 mg/mL), there was no significant production of this cytokine, which may be the result of the regulation of IL-10, since significant production of NO was observed in the periods of 24 and 48 hours in macrophages exposed to the extract [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

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In Vitro Modulator Effect of Total Extract from the Endophytic Paenibacillus polymyxa RNC-D in Leishmania (Leishmania) amazonensis and Macrophages

Neris

Ortolani

Castro

et al. 2020

International Journal of Microbiology

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Leishmaniases are diseases with high epidemiological relevance and wide geographical distribution. In Brazil, Leishmania (Leishmania) amazonensis is related to the tegumentary form of leishmaniasis. The treatment for those diseases is problematic as the available drugs promote adverse effects in patients. Therefore, it is important to find new therapeutic targets. In this regard, one alternative is the study of biomolecules produced by endophytic microorganisms. In this study, the total extract produced by the endophytic Paenibacillus polymyxa RNC-D was used to evaluate the leishmanicidal, nitric oxide, and cytokines production using RAW 264.7 macrophages. The results showed that, in the leishmanicidal assay with L. amazonensis, EC50 values at the periods of 24 and 48 hours were 0.624 mg/mL and 0.547 mg/mL, respectively. Furthermore, the cells treated with the extract presented approximately 25% of infected cells with an average of 3 amastigotes/cell in the periods of 24 and 48 hours. Regarding the production of cytokines in RAW 264.7 macrophages infected/treated with the extract, a significant increase in TNF-α was observed at the periods of 24 and 48 hours in the treated cells. The concentrations of IFN-γ and IL-12 showed significant increase in 48 hours. A significant decrease in IL-4 was observed in all cells treated with the extract in 24 hours. It was observed in the treated cells that the NO production by RAW 264.7 macrophages increased between 48 and 72 hours. The endophytic Paenibacillus polymyxa RNC-D extract modulates the mediators of inflammation produced by RAW 264.7 macrophages promoting L. amazonensis death. The immunomodulatory effects might be a promising target to develop new immunotherapeutic and antileishmanial drugs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%