Cytotoxic effects of acrylic acid, methacrylic acid, their corresponding saturated carboxylic acids, HEMA, and hydroquinone on fibroblasts derived from human pulp

Kurata, Shigeaki; Morishita, Kumiko; Kawase, Toshio; Umemoto, Kozo

doi:10.4012/dmj.2011-085

Cited by 31 publications

(32 citation statements)

References 23 publications

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“…Literature reports that monomers such as HEMA are soluble in aqueous medium (31) and are present in adhesive systems such as Single Bond (11). However, under the conditions of the present study, HEMA did not alter the cell viability or metabolism even in direct contact with human dental pulp fibroblasts at 1 nM, in contrast with other works that report decrease of fibroblast grow in HEMA treatment groups (5 mmol/L and and 3 mmol/L) compared to control group (32). The concentrations tested in the present study do not necessarily correspond to the ones present in resin systems, since these data are unavailable from manufacturer's information.…”

Section: Discussioncontrasting

confidence: 97%

Dental Pulp Fibroblasts Response after Stimulation with HEMA and Adhesive System

et al. 2018

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This study evaluated in vitro cell viability and metabolism, nitric oxide release and production of chemokines by cultured human dental pulp fibroblasts (DPF) under contact with HEMA and Single Bond. Cultures of DPF were established by means of an explant technique. Once plated, cells were kept under contact with increasing concentrations of HEMA (10, 100 and 1000 nM) or Single Bond (SB) [10-fold serially diluted in culture medium (10 -4 , 10 -3 and 10 -2 v/v)] and also with polymerized SB components. Cytotoxicity was assessed by Trypan Blue exclusion method and MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. Nitric oxide release on cell supernatant was detected by Griess Method whereas chemokines (CXCL12 and CXCL8) were detected by ELISA. RT-qPCR was employed for chemokines gene expression analysis. Cytotoxic tests showed significant differences for SB 10 -2 . None of the tested materials significantly altered NO levels. Protein levels of CXCL12 were significantly decreased only by HEMA. On the other hand, while CXCL12 mRNA remained unaltered, gene expression of CXCL8 had significant decrease with all materials, except for polymerized SB. In conclusion, Single Bond and HEMA at various concentrations, decreased expression and production of molecules involved in inflammatory processes and, therefore, the use of adhesive systems such as pulp capping materials must be viewed with caution due to its large cytotoxic effect when in close contact with the pulp.

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Section: Discussioncontrasting

confidence: 97%

Dental Pulp Fibroblasts Response after Stimulation with HEMA and Adhesive System

et al. 2018

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“…Although the cell viability of UVB-irradiated dA did not totally consist of the concentration of the produced hydroquinone, it might because the original dA has activity to enhance the growth of Detroit 551 cells (Figure 1b). In addition, the cytotoxic effects of HQ to fibroblasts had been estimated by previous study; fibroblast growth of 3 to 5 mM HQ after two days markedly decreased compared with that of control, only 10% to 40% cells stayed alive [15]. Therefore, we can propose again that the severe cytotoxicity of UVB-irradiated dA to fibroblasts is mainly provided by HQ.…”

Section: Resultsmentioning

confidence: 59%

Study of Hydroquinone Mediated Cytotoxicity and Hypopigmentation Effects from UVB-Irradiated Arbutin and DeoxyArbutin

Chang

Chen

Lin

et al. 2017

IJMS

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Arbutin (Arb) and deoxyArbutin (dA) are both effective hypopigmentation agents. However, they are glucoside derivatives of hydroquinone (HQ), which may be decayed into HQ under higher energy environments. Therefore, safety and toxicity are very important issues when considering the usage of these compounds. However, no study has verified the properties of Ultra-Violet B (UVB)-irradiated Arb and dA. In this work, we investigated the cytotoxicity and hypopigmentation effects of UVB-irradiated Arb and dA in Detroit 551 human fibroblast cells and B16-F10 mouse melanoma cells. The results showed that UVB-irradiated Arb and dA have strong cytotoxicity for the fibroblast cells, especially for dA, the caspase-3 is also activated by the treatment of UVB-irradiated dA in Detroit 551 cells. The results correlated with the produced HQ. In addition, UVB-irradiated Arb and dA suppressed the production of melanin in melanoma cells; this is due to the release of HQ that compensates for the UVB triggered Arb and dA decomposition.

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“…Nevertheless, it can be supposed that the presence of MMA in Durabase Soft liquid contributed, at least in part, to the cytotoxic effects observed in the present study. Moreover, MMA can decompose via hydrolysis in methacrylic acid (MA) [35], which also has a cytotoxic effect [36], altering both cellular metabolism and DNA synthesis [37]. …”

Section: Discussionmentioning

confidence: 99%

Effects of Soft Denture Liners on L929 Fibroblasts, HaCaT Keratinocytes, and RAW 264.7 Macrophages

Chaves

Costa

Vergani

et al. 2014

BioMed Research International

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The effects of six soft liners (Ufi Gel P (UG), Sofreliner S (SR), Durabase Soft (D), Trusoft (T), Coe Comfort (CC), and Softone (ST)) on L929, HaCat, and RAW 264.7 cells were investigated. Eluates (24 and 48 h) from the materials were applied on the cells and the viability, type of cell death, and morphology were evaluated. Cells were also seeded on the specimens' surfaces (direct contact) and incubated (24 or 48 h), and viability was analyzed. Controls were cells in culture medium without eluates or specimens. For cell viability, no significant differences were found among materials or between extraction periods, and the liners were noncytotoxic or slightly cytotoxic. Morphology of RAW 264.7 cells was altered by the 24 h eluates from CC and D and the 48 h eluates from SR, CC, and D. The 24 and 48 h eluates from all materials (except T) increased the percentages of L929 necrotic cells. For direct contact tests, the lowest cytotoxicity was observed for UG and SR. Although eluates did not reduce viability, morphology alterations and increase in necrosis were seen. Moreover, in the direct contact, effects on viability were more pronounced, particularly for D, T, CC and ST. Thus, the use of UG and SR might reduce the risk of adverse effects.

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Cytotoxic effects of acrylic acid, methacrylic acid, their corresponding saturated carboxylic acids, HEMA, and hydroquinone on fibroblasts derived from human pulp

Cited by 31 publications

References 23 publications

Dental Pulp Fibroblasts Response after Stimulation with HEMA and Adhesive System

Dental Pulp Fibroblasts Response after Stimulation with HEMA and Adhesive System

Study of Hydroquinone Mediated Cytotoxicity and Hypopigmentation Effects from UVB-Irradiated Arbutin and DeoxyArbutin

Effects of Soft Denture Liners on L929 Fibroblasts, HaCaT Keratinocytes, and RAW 264.7 Macrophages

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