Cytotoxic Effects of Activated Human Monocytes and Lymphocytes to Anti‐D‐treated Human Erythrocytes in Vitro

Randazzo, B.; Hirschberg, Tom; Hirschberg, Henry

doi:10.1111/j.1365-3083.1979.tb03173.x

Cited by 13 publications

(2 citation statements)

References 15 publications

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“…The following variation in technique could be considered: pure monocytes as effector cells [6], enzyme treatment of target cells [24], addition of cytochalasin B to the effector cell-target cell mixture [8], determination of the osmotic fragility of the target cells in addition to lysis [7] and prior activation of effector cells by incubation with cell-free supernatants from mixed lymphocytic cultures [28], Spontaneous cell-mediated cytotoxicity as discussed by Leibold and Peter [21 ] should also be considered. Another possibility would be to care fully search for IgA antibodies.…”

Section: Discussionmentioning

confidence: 99%

Intravascular Hemolytic Transfusion Reaction without Detectable Antibodies: A Case Report and Review of Literature

Harrison¹,

Hayes²,

Trow³

et al. 1986

Vox Sanguinis

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A case of intravascular hemolytic transfusion reaction without detectable antibodies occurring in a 55-year-old male is reported. Specificity for the C antigen in the Rh system was demonstrated by technetium-99m red cell survival studies. A cell-mediated mechanism of hemolysis was suspected and investigated. Previously reported cases are reviewed and discussed. The entity of intravascular hemolytic transfusion reaction associated with minimal symptoms and no detectable antibodies deserves further investigation.

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Section: Discussionmentioning

confidence: 99%

Intravascular Hemolytic Transfusion Reaction without Detectable Antibodies: A Case Report and Review of Literature

Harrison¹,

Hayes²,

Trow³

et al. 1986

Vox Sanguinis

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“…Human monocytes and neutrophils are able to lyse a variety of antibody-sensitized target cells in vitro, including tumor cell lines and erythrocytes (RBC) (9,14,22,23,27,29,37). This has led to the suggestion that antibody-dependent cell-mediated cytotoxicity (ADCC) may be important in tumor surveillance (12, 15, 38) as well as in the pathogenesis of hemolytic anemias.…”

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confidence: 99%

Activation of Monocyte and Granulocyte Antibody-Dependent Cytotoxicity by Phorbol Myristate Acetate

1982

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We have characterized the effects of phorbol myristate acetate (PMA) on human monocyte and neutrophil oxidative metabolism and antibody-dependent cytotoxicity toward anti-D sensitized human erythrocytes (RBC) and a human lymphoblastoid cell line (CEM). Hexose monophosphate shunt activity was measured by [1- 14 C]glucose oxidation and target lysis by 51 Cr release. PMA produced a dose-dependent stimulation of hexose monophosphate shunt activity. Neutrophils responded with higher hexose monophosphate shunt activity and at a lower PMA concentration than did monocytes. PMA increased monocyte lysis of antibody-sensitized RBC by two-thirds, but did not affect lysis of CEM targets. Neutrophils were unable to lyse either antibody-sensitized or nonsensitized RBC without the addition of PMA. When PMA was added, lysis of both targets increased markedly. Neutrophils without PMA were able to lyse a small number of both antibody-sensitized and nonsensitized CEM targets. PMA also increased neutrophil lysis of these targets. Target lysis by neutrophils from a patient with chronic granulomatous disease, cells unable to produce reactive oxygen species, was not increased by PMA. Chronic granulomatous disease monocytes, however, responded to PMA by more than doubling lysis of antibody-sensitized RBC. Hypoxia inhibited PMA augmentation of antibody-sensitized RBC lysis by neutrophils, but not by monocytes. Generation of reactive oxygen species by the xanthine-xanthine oxidase system inhibited CEM growth, but did not cause lysis, indicating that in some cases oxidative injury may be nonlytic. We suggest that PMA augments neutrophil cytotoxicity to tumor and RBC targets by stimulating reactive oxygen species-mediated lysis, but in monocytes augmentation of lysis is due to activation of a nonoxidative mechanism of lysis.

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Monocyte-mediated-antibody-dependent cellular cytotoxicity in malignant lymphoma and solid tumors

Mulder¹,

Pauw²,

Ven³

et al. 1984

Cancer

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Monocyte‐mediated‐antibody‐dependent cellular cytotoxicity (MO‐ADCC) was studied in 21 patients with Hodgkin's disease (HD), 15 patients with a long‐lasting remission of HD, 11 patients with non‐Hodgkin's lymphoma (NHL), 11 patients with solid tumors, and 15 normal controls. Lymphocyte ADCC (LY‐ADCC) was evaluated in 12 patients with HD and 9 normal controls. Monocytes and lymphocytes were isolated with cell‐scatter monitored counterflow centrifugation providing high purity and yield. Antibody‐dependent cellular cytotoxicity was evaluated by means of DNA flowcytometry, using antibody‐coated chicken erythrocyte targets (CRBC). in comparison with normal controls MO‐ADCC was significantly increased in HD (P <0.0005), NHL (P < 0.005), and solid tumors (P < 0.005). in patients in a long‐lasting complete remission of HD, MO‐ADCC was in the normal range. Lymphocyte‐ADCC of 12 patients with HD was similar to that of 9 normal controls. in all experiments LY‐ADCC was invariably lower than MO‐ADCC of the same donor, indicating the monocyte as the most potent effector cell towards CRBC targets. Results indicate the following: (1) purified cell suspensions of both lymphocytes and monocytes are essential to unravel their role as effector cells; (2) LY‐ADCC in HD is similar to normal controls; (3) MO‐ADCC enhancement is not uncommon in malignant lymphoma and several solid tumors; (4) normal MO‐ADCC in a group of successfully treated patients with HD suggests a disease‐related induction of enhanced MO‐ADCC.

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Cytotoxic Effects of Activated Human Monocytes and Lymphocytes to Anti‐D‐treated Human Erythrocytes in Vitro

Cited by 13 publications

References 15 publications

Intravascular Hemolytic Transfusion Reaction without Detectable Antibodies: A Case Report and Review of Literature

Intravascular Hemolytic Transfusion Reaction without Detectable Antibodies: A Case Report and Review of Literature

Activation of Monocyte and Granulocyte Antibody-Dependent Cytotoxicity by Phorbol Myristate Acetate

Monocyte-mediated-antibody-dependent cellular cytotoxicity in malignant lymphoma and solid tumors

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