Cytotoxic Effects of Air Freshener Biocides in Lung Epithelial Cells

Kwon, Junhyun; Lee, Mimi; Seo, Gun-Baek; Kim, Hyun-Mi; Shim, Ilseob; Lee, Doo-Hee; Kim, Taksoo; Seo, Jung Kwan; Kim, Pilje; Choi, Kyunghee

doi:10.1177/1934578x1300800929

Cited by 6 publications

(6 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To date, TCS-stimulated ROS production has been poorly studied in mammalian cells. The only available data refer to the A549 cell line, rat embryonal stem cells and mouse neuronal cells [14, 16, 32].…”

Section: Discussionmentioning

confidence: 99%

“…However, TCS-stimulated ROS production has been poorly studied in mammalian cells. The only available data refer to the human lung epithelial (A549) cell line, rat embryonal stem cells and mouse neuronal cells [14, 16, 32]. Furthermore, up to today, no studies have been undertaken to investigate the involvement of NMDARs or ROS-dependent excitotoxicity in the nervous system’s response to TCS.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Triclosan-Evoked Neurotoxicity Involves NMDAR Subunits with the Specific Role of GluN2A in Caspase-3-Dependent Apoptosis

et al. 2018

View full text Add to dashboard Cite

Triclosan (TCS) is an antimicrobial agent that is used extensively in personal care and in sanitising products. A number of studies have shown the presence of TCS in different human tissues such as blood, adipose tissue, the liver, brain as well as in breast milk and urine. N-Methyl-D-aspartate receptors (NMDARs) are glutamate-gated ion channels that are widely expressed in the central nervous system and which play key roles in excitatory synaptic transmission. There is, however, no data on the involvement of NMDAR subunits in the apoptotic and neurotoxic effects of TCS. Our experiments are the first to show that TCS used at environmentally relevant concentrations evoked NMDA-dependent effects in neocortical neurons in primary cultures, as MK-801, an uncompetitive NMDA receptor antagonist, reduced the levels of TCS-induced ROS production as well as caspase-3 activity and LDH release. TCS caused a decrease in protein expression of all the studied NMDA receptor subunits (GluN1, GluN2A, GluN2B) that were measured at 3, 6 and 24 h post-treatment. However, at 48 h of the experiment, the level of the GluN1 subunit returned to the control level, and the levels of the other subunits showed a tendency to increase. In TCS-treated neocortical cells, protein profiles of NMDAR subunits measured up to 24 h were similar to mRNA expression of GluN1 and GluN2A, but not to GluN2B mRNA. In this study, cells transiently transfected with GluN1, GluN2A or GluN2B siRNA exhibited reduced levels of LDH release, which suggests the involvement of all of the studied NMDAR subunits in the neurotoxic action of TCS. According to our data, GluN1 and GluN2A were mainly responsible for neuronal cell death as evidenced by neutral red uptake, whereas GluN2A was involved in TCS-induced caspase-3-dependent apoptosis. We suggest that TCS-evoked apoptosis and neurotoxicity could be related to transient degradation of NMDAR subunits in mouse neurons. Furthermore, recycling of NMDAR subunits in response to TCS is possible. Because transfections with specific siRNA did not completely abolish the effects of TCS as compared to cells transfected with negative siRNA in this study, other NMDAR-independent mechanisms of TCS action are also possible.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Triclosan-Evoked Neurotoxicity Involves NMDAR Subunits with the Specific Role of GluN2A in Caspase-3-Dependent Apoptosis

et al. 2018

View full text Add to dashboard Cite

show abstract

“…According to environmental studies, chronic use of air fresheners by inhalation or ingestion is harmful for all living organisms. These products have endocrine disrupting phthalates (di-cyclohexyl phthalate, di-isononyl phthalate, and di-n-propyl phthalate) and cytotoxic effects [19,20]. Environmentalists advise to periodically change the brands and flavors of air fresheners used to avoid chronic exposures [21].…”

Section: Discussionmentioning

confidence: 99%

Forensic Identification of Air Freshener Components from the Toxicological Samples by GC-MS : A Case Report

Mukharjee¹,

Yadav²,

Singh³

2020

AJFSFM

View full text Add to dashboard Cite

Indoor air fresheners are commonly used to deodorize rooms and cars. A case of air freshener intoxication by oral ingestion was forwarded to the forensic laboratory to determine the components and cause of death. The presumptive tests were conducted for organo-phosphorus and organo-chloro compounds, formaldehyde, carbolic acid, drugs and aromatic compounds. Gas Chromatography–Mass Spectroscopy (GC-MS) was used as a confirmatory test for all these compounds. Presence of toxic substances like dichlorvos (2,2 di-chlorovinyl dimethyl phosphate), phenol, formaldehyde, naphthalene was confirmed in the air freshener that led to the person’s death. Laboratory analysis also confirmed the presence of alprazolam in the viscera as per case history. The methodology used can be utilized as a reference for TLC and GC-MS based analysis of such cases

show abstract

“…BP-3 may also disrupt the levels of PPARγ receptor, down-regulating surfactant protein expression in alveolar type II cells 9 , essential for lung maturation. Triclosan was shown to decrease the viability, growth and morphology of lung epithelial cells 67 . Parabens, triclosan, and BP-3 can also increase biomarkers of oxidative stress and inflammation 68 .…”

Section: Parabens Triclosan and Benzophenonesmentioning

confidence: 97%

In Utero Exposure to Organic Pollutants and Lung Function in the Offspring

Abellan¹,

Casas²

2021

BRN

View full text Add to dashboard Cite

Humans are exposed daily to thousands of chemicals present in many consumer products that can interfere with hormonal signalling systems. The prenatal period is critical because the developing lung is intrinsically subject to hormonal regulation. Alterations at this time may predispose to reduced lung function in later life. In this review, we summarise current knowledge about the role of prenatal exposure to organic pollutants on lung function in the offspring. We divide pollutants into persistent: organochlorine and perfluoroalkyl compounds, and non-persistent: bisphenols, parabens, triclosan, benzophenones, phthalates, and currently used pesticides. Eleven prospective cohort studies, mainly from Europe and the US, have been identified. Overall, the literature is scarce and inconsistent. The observed associations have identified small changes in lung function parameters. Main challenges for future studies include assessment of exposure to non-persistent pollutants and the study of multipollutant effects. In parallel, public health strategies should be implemented to reduce exposure to organic pollutants, particularly in pregnant women.

show abstract

Cytotoxic Effects of Air Freshener Biocides in Lung Epithelial Cells

Cited by 6 publications

References 17 publications

Triclosan-Evoked Neurotoxicity Involves NMDAR Subunits with the Specific Role of GluN2A in Caspase-3-Dependent Apoptosis

Triclosan-Evoked Neurotoxicity Involves NMDAR Subunits with the Specific Role of GluN2A in Caspase-3-Dependent Apoptosis

Forensic Identification of Air Freshener Components from the Toxicological Samples by GC-MS : A Case Report

In Utero Exposure to Organic Pollutants and Lung Function in the Offspring

Contact Info

Product

Resources

About