Cytotoxic effects of Annona squamosa leaves against breast cancer cells via apoptotic signaling proteins

Al-Nemari, Rawan; Bacha, Abir Ben; Al-Senaidy, Abdulrahman M.; Almutairi, Mikhlid H.; Arafah, Maha; Alsaran, Hadel; Abutaha, Nael; Semlali, Abdelhabib

doi:10.1016/j.jksus.2022.102013

Cited by 13 publications

(7 citation statements)

References 20 publications

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“…Collagenase type IV belongs to the MMP groups, destroys the epithelial basement membrane and vascular membranes, and releases ECM growth factors. After local destruction of the basement membrane and ECM, a migration channel will form, resulting in the migration of tumor cells so that tumor cells can enter the vessels and be carried by the bloodstream or lymph flow [9], [23].…”

Section: Discussionmentioning

confidence: 99%

Annona Squamosa Leaf Extract Inhibits the Migration of Human Cervical Cancer Cells Through MMP-9 Expression

2023

Journal of Southwest Jiaotong University

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To determine the effect of giving a combination of Annona squamosa leaf extract and cisplatin on matrix metalloproteinase-9 expression and inhibition of cell migration in HeLa cells. The sample in this study was divided into 6 groups: group 1 as the control group, group 2 as the single cisplatin (Cis) group, group 3-5 as the different combinations with ASL 12.5, 25 mg/mL, and 50 mg/mL respectively, and group 6 as the single ASL 75 mg/mL group. This research included the cytotoxicity test with MTT assay and MMP-9 expression using immunofluorescence method cell migration with scratch wound healing assay. In this study, the expression of MMP-9 decreased significantly in Annona squamosa leaf extract 25 mg/mL + Cis. The cytotoxicity activity of Annona squamosa leaf extract increased significantly in the ASL 12.5+Cis group. The combination of ASL 25+Cis significantly inhibited cell migration. The combination extract was more effective than a single dose of Cis or a single dose of the extract alone (p < 0.05). The novelty of this study can be used for combined cervical cancer therapy. The combination of cisplatin with an extract with the active ingredient acetogenin can induce cytotoxicity and reduce MMP-9 expression in HeLa cells. Acetogenins are also found in bark, seed, and fruit extracts; however, the

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Section: Discussionmentioning

confidence: 99%

Annona Squamosa Leaf Extract Inhibits the Migration of Human Cervical Cancer Cells Through MMP-9 Expression

2023

Journal of Southwest Jiaotong University

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“…For instance, aqueous extracts of Carica papaya leaves were found to inhibit the viability of K562 tumor cells ( Otsuki et al, 2010 ). Al-Nemari et al (2022) demonstrated that the leave extracts of Annona squamosa could effectively inhibit MCF-7 and MDA-MB-231 tumor cells. Moreover, acidic polysaccharides from Gynostemma pentaphyllum have been found to possess significant inhibitory activities against SPC-A-1 and MGC-803 cells ( Yu et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the in vitro antioxidant and antitumor activity of extracts from Camellia fascicularis leaves

Peng

He²,

Tang³

et al. 2022

Front. Chem.

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Camellia fascicularis is a unique plant rich in bioactive components. However, the isolation of the active substances in C. fascicularis leaves via sequential extraction with solvents of different polarity and the determination of their antioxidant and antitumor activities have not been reported. In this study, the total methanol extract of C. fascicularis leaves was sequentially extracted with different polar solvents, and the corresponding petroleum ether extract (PEE), ethyl acetate extract (EAE), and water extract (WE) were analyzed for their contents in active substances such as flavonoids, polyphenols, polysaccharides, and saponins. The antioxidant ability of the polar extracts was investigated by determining their reducing power and the radical scavenging rate on 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and hydroxyl radicals, and CCK-8 and Annexin-FITC/propidium iodide staining assays were conducted to investigate their inhibitory effects on HCCLM6 and HGC27 tumor cells. The results showed that PEE had a high saponin content of 197.35 ± 16.21 mg OAE/g, while EAE and WE exhibited a relatively higher polysaccharide content of 254.37 ± 1.99 and 373.27 ± 8.67 mg GE/g, respectively. The EAE demonstrated the greatest reducing power and the strongest clearing abilities on ABTS and DPPH radicals with respective EC50 values of 343.45 ± 20.12 and 14.07 ± 0.06 μg/ml. Moreover, the antitumor ability of the different polar extracts was dose-dependent, with WE showing the most potent inhibitory ability against HCCLM6 and HGC27 cells.

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“…They showed potent cytotoxicity, increasing caspase-3 expression significantly and cell cycle arrest in the G2/M phase. According to Al-Nemari et al, 2022, the free A. squamosa leaf extract increased the expression of proliferative and apoptotic markers, which in turn enhanced the extract’s anticancer effect on breast cancer cell lines.…”

Section: Introductionmentioning

confidence: 99%

“…They showed potent cytotoxicity, increasing caspase-3 expression significantly and cell cycle arrest in the G2/M phase. According to Al-Nemari et al, 2022, 14 the free A. squamosa leaf extract increased the expression of proliferative and apoptotic markers, which in turn enhanced the extract’s anticancer effect on breast cancer cell lines. Additionally, they conducted an in vivo investigation, and it was found that the treatment of rats with the extracts had an impact on the tumor size, proliferative, and apoptotic indicators.…”

Section: Introductionmentioning

confidence: 99%

Annona squamosa L. Extract-Loaded Niosome and Its Anti-Ehrlich Ascites’ Carcinoma Activity

et al. 2022

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Current research is focused on cancer treatments other than chemotherapy medications, particularly those derived from natural sources. The goal of this work was to look at the anticancer and biomarker properties of a methanolic extract of Annona squamosa leaves and their extract-loaded noisome. A. squamosa leaves extract and their leaves extract-loaded noisome were prepared. Transmission electron microscopy was used to screen the size of the niosomes loaded with the A. squamosa L. leaves extract. The tumor size, blood picture (hemoglobin, red blood cells, white blood cells), liver functions, kidney function, oxidative stress, and inflammatory markers were evaluated to assess the potential anticancer activity of the A. squamosa leaves extract and A. squamosa leaves extract-loaded noisome in Ehrlich ascites carcinoma. A. squamosa L. leaves extract was found to be an effective anticancer treatment. The protective effect of the loaded extract showed more significant results. All treated groups showed a lower tumor volume compared to the positive control. Liver and kidney functions were improved, and inflammatory markers were decreased. Oxidative stress was improved in tumor, liver, and kidney tissues. A. squamosa leaves contain major anticancer compounds that in general help most enzymes of the liver and kidney and other injured organs to return to their normal levels.

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Cytotoxic effects of Annona squamosa leaves against breast cancer cells via apoptotic signaling proteins

Cited by 13 publications

References 20 publications

Annona Squamosa Leaf Extract Inhibits the Migration of Human Cervical Cancer Cells Through MMP-9 Expression

Annona Squamosa Leaf Extract Inhibits the Migration of Human Cervical Cancer Cells Through MMP-9 Expression

Evaluation of the in vitro antioxidant and antitumor activity of extracts from Camellia fascicularis leaves

Annona squamosa L. Extract-Loaded Niosome and Its Anti-Ehrlich Ascites’ Carcinoma Activity

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