Cytotoxic effects of chemopreventive agents curcumin, naringin and epigallocathecin-3-gallate in C2C12 myoblast cells

Abstract

Muscle tissues make up about 40-50% of the human bodies. Satellite cells, which present in between the basal lamina and myofiber, are the adult muscle stem cells or myoblasts which are important for the regeneration of muscle tissues. Anticancer agents generally possess high cytotoxicity to either cancer cells or normal cells. Their effects on muscle cells generate cachexia or the deterioration of muscle tissues. Chemopreventive agents which possess lower cyctotoxic effects are expected to show higher safety in normal cells. Therefore, we investigated the effects of chemopreventive agents curcumin, naringin, and epigallocathecin-3-gallate (EGCG), which show anticancer properties in cancer cells, in C2C12 myoblast cells. We observed the C2C12 cell viability by MTT and WST assays, cell migration by wound healing scratch assay, as well as differentiation assay after treatment with the chemopreventive agents. The results indicated that curcumin showed highest cytotoxicity compared to naringin and EGCG. In addition, naringin and EGCG exhibited lower cytotoxicity. Both naringin and EGCG inhibited C2C12 cell migration at cell density 150, 000 cells/ml. Whereas, at cell density 100, 000 cells/ml, there was no significant effects of naringin as well as EGCG. Altogether, the results suggest that naringin and EGCG possess lower cytotoxic effect on C2C12 myoblast cells whereas curcumin showed stronger cytotoxicity at concentration higher than 20 µM.

Cytotoxicity of curcumin-loaded magnetic nanoparticles against normal and cancer cells as a breast cancer drug delivery system

“…After incubation for 2 hours, the formazan crystals as a result of the transformation of MTT, were solubilized by DMSO in 5 minutes. Using a microplate reader (BioTek 800TS, USA ), the absorbance of the color caused by the generation of solubilized crystals was quantified at 570 nm (Septisetyani, Santoso, Wisnuwardhani, & Prasetyaningrum, 2020). The amount of living cells in the culture is directly related to the absorption reading.…”