Cytotoxic Effects of Compounds Isolated from Ricinodendron heudelotii

Yakubu, Omolara Faith; Adebayo, Abiodun Humphrey; Dokunmu, Titilope M.; Zhang, Yingjun; Iweala, E. E. J.

doi:10.3390/molecules24010145

Cited by 14 publications

(4 citation statements)

References 41 publications

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“…Compound 10 showed two molecular ion peaks at m/z 634.9866 [M-H] - and 1271.1479 [2M-H] - . After 1 H NMR analysis, the molecular formula was deduced as C 27 H 24 O 18 and was identified as 1, 2, 6, tri- O -galloyl β-D-glucose ( Fig 5.10 )after comparing it with published literature [ 43 ]. Compound 11 showed two molecular ion peaks at m/z 953.5759 [M-H] - and 1907.0813 [2M-H] - .…”

Section: Resultsmentioning

confidence: 99%

Extraction, phytochemical characterization and anti-cancer mechanism of Haritaki churna: An ayurvedic formulation

2023

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Haritaki churna (HC), a single herb ayurvedic formulations is known to be prescribed for various gastro-intestinal disorders in Ayurveda. Haritaki churna aqueous extract (HCAE) has anti-cancer activity against different types of cancer cells with an IC50 in the range of 50–97 μg/ml. Bioavailability of Haritaki Churna is very high in digestive track and treatment of colorectal cancer cells HCT-116, DLD1, HT-29 with HCAE reduces its cellular viability with anti-cancer IC50 70μg/ml. HCAE consumption is safe for human as it didn’t affect the cellular viability of primary human PBMCs or non-cancerogenic HEK-293 cells. Haritaki churna was found to be stable in biological gastric fluids and bioactive agents are not losing their anti-cancer activity under such harsh conditions. The HPLC Chromatogram of HCAE is giving 13 major peaks and 11 minor peaks. Exploiting LC-MS, IR and NMR spectroscopic techniques, a total of 13 compounds were identified from HCAE namely Shikimic acid, Chebulic acid, gallic acid, 5-hydroxymethylfurfural, Protocatechuic acid, 4-O-galloyl-shikimic Acid, 5-O-galloyl-shikimic Acid, Methylgallate, corilagin, 1, 2, 6, Tri-O-galloyl β-D-glucose, chebulagic acid, chebulinic acid, and Ellagic acid. Reconstitution and subtraction of phytochemicals from the mixture indicate that Ellagic acid significantly contribute into anti-cancer effect of HCAE. Cancer cells treated with ellagic acid from HCAE were incapable of completing their cell-cycle and halted the cell-cycle at DNA synthesis S-Phase, as demonstrated by decreased cyclin A2 expression levels with increasing ellagic acid concentration. Halting of cells at S-phase causes induction of apoptosis in cancer cells. Cancer cells exhibiting DNA fragmentation, changes in expression of several apoptotic proteins such as Bcl2, cytochrome-c and formation of cleaved products of caspase 3 and PARP-1 suggests ellagic acid induces cell death via mitochondrial pathway of apoptosis.

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Section: Resultsmentioning

confidence: 99%

Extraction, phytochemical characterization and anti-cancer mechanism of Haritaki churna: An ayurvedic formulation

2023

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“…Bioactive compounds which are naturally obtained from plants have been recognised for their therapeutic potential in the treatment of different types of cancers [18][19][20][21][22]. These According to the World Health Organization, the classification of haematological malignancies into different categories is based on the origin of the uncontrollably differentiating cell, the stage of disease development and clinical features of the condition [4,5].…”

Section: Introductionmentioning

confidence: 99%

“…Bioactive compounds which are naturally obtained from plants have been recognised for their therapeutic potential in the treatment of different types of cancers [18][19][20][21][22]. These naturally sourced products, which are sometimes referred to as herbs or traditional medicines, have been recognised for their roles as chemotherapeutic adjuvants [9,23,24].…”

Section: Introductionmentioning

confidence: 99%

Bioactive Phytoconstituents and Their Therapeutic Potentials in the Treatment of Haematological Cancers: A Review

Iweala

Oluwapelumi

Dania

et al. 2023

Life

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Haematological (blood) cancers are the cancers of the blood and lymphoid forming tissues which represents approximately 10% of all cancers. It has been reported that approximately 60% of all blood cancers are incurable. Despite substantial improvement in access to detection/diagnosis, chemotherapy and bone marrow transplantation, there is still high recurrence and unpredictable but clearly defined relapses indicating that effective therapies are still lacking. Over the past two decades, medicinal plants and their biologically active compounds are being used as potential remedies and alternative therapies for the treatment of cancer. This is due to their anti-oxidant, anti-inflammatory, anti-mutagenic, anti-angiogenic, anti-cancer activities and negligible side effects. These bioactive compounds have the capacity to reduce proliferation of haematological cancers via various mechanisms such as promoting apoptosis, transcription regulation, inhibition of signalling pathways, downregulating receptors and blocking cell cycle. This review study highlights the mechanistic and beneficial effects of nine bioactive compounds (quercetin, ursolic acid, fisetin, resveratrol, epigallocatechin gallate, curcumin, gambogic acid, butein and celastrol) as potential remedies for chemoprevention of haematological cancers. The study provides useful insights on the effectiveness of the use of bioactive compounds from plants for chemoprevention of haematological cancers.

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“…The bark helps cure malaria, yellow fever, and dysentery and equally has diuretic functions [7]. Recently, we reported the antimicrobial effect of R. heudelotii extract [8] and the cytotoxic effect of isolated compounds from the leaf extract [9]. It was also reported that the leaf extract exhibited regulatory role on the toxicity of artemisinin when co-administered with artemisinin [10].…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory and antioxidant activities of fractions and compound from Ricinodendron heudelotii (Baill.)

Yakubu

Adebayo

Iweala

et al. 2019

Heliyon

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Medicinal plants have been documented over the years to play vital role in promoting human health. The study evaluated the anti-inflammatory and anti-oxidant activities of different fractions and isolated compound from Ricinodendron heudelotii leaves. The leaves of Ricinodendron heudelotii were extracted with ethanol and further partitioned sequentially using petroleum ether, ethylacetate and butanol. Bioassay–guided fractionation of the ethylacetate fraction was done using repeated column chromatographic technique while the structural elucidation of pure compound was carried out using mass spectra, 13C and 1H NMR analyses. Antioxidant potential of the fractions and isolated compound were evaluated with 2,2-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays and anti-inflammatory effect of fractions was measured by their inhibitory potency on nitric oxide (NO).Corilagin, an amorphous tannin was isolated and structurally elucidated. Corilagin showed scavenging effect against ABTS and DPPH radicals which vary in a dose dependent manner. It also showed an antioxidant potential with IC50 value of 0.003 mg/mL comparable to vitamin C 0.001 mg/mL) used as standard. The butanol and ethylacetate fractions exhibited significant (p < 0.05) NO inhibition of 60 and 69% respectively after treatment of RAW 264.7 macrophages with lipopolysaccharide. These results demonstrated the role of isolated corilagin as a promising potent antioxidant while the ethylacetate and butanol fractions suppressed the expression of an inflammation mediator by inhibiting nitric oxide.

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Cytotoxic Effects of Compounds Isolated from Ricinodendron heudelotii

Cited by 14 publications

References 41 publications

Extraction, phytochemical characterization and anti-cancer mechanism of Haritaki churna: An ayurvedic formulation

Extraction, phytochemical characterization and anti-cancer mechanism of Haritaki churna: An ayurvedic formulation

Bioactive Phytoconstituents and Their Therapeutic Potentials in the Treatment of Haematological Cancers: A Review

Anti-inflammatory and antioxidant activities of fractions and compound from Ricinodendron heudelotii (Baill.)

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