Cytotoxic effects of dental cements on two cell culture systems

Hanks, C. T.; Anderson, Marlene; Craig, Robert G.

doi:10.1111/j.1600-0714.1981.tb01255.x

Cited by 96 publications

(57 citation statements)

References 24 publications

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“…It demonstrates the importance of the extraction medium used in toxicity testing of dental resins. It can explain contradictory results obtained from cytotoxicity assays when different extraction media were used, especially when concentration of TEGDMA monomer released into ethanol solution is compared to distilled water [30,31,36].…”

Section: Discussionmentioning

confidence: 92%

Elution study of unreacted TEGDMA from bulk-fill composite (SDR™ Dentsply) using HPLC

Łagocka

Jakubowska

Chlubek

et al. 2015

Advances in Medical Sciences

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Section: Discussionmentioning

confidence: 92%

Elution study of unreacted TEGDMA from bulk-fill composite (SDR™ Dentsply) using HPLC

Łagocka

Jakubowska

Chlubek

et al. 2015

Advances in Medical Sciences

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“…Different zinc phosphate cements have been revealed to be highly cytotoxic immediately after mixing. 17 Completely set specimens that were eluted in 0.9% saline solution or in cell culture medium for 7 days were not cytotoxic (cell culture medium) or were only slightly cytotoxic (saline solution) in cultures of periodontal ligament fibroblasts. Toxic reactions were observed 4 in a permanent growing cell line (3T3 mouse fibroblasts).…”

Section: Discussionmentioning

confidence: 95%

Cytotoxic effects of resin-modified orthodontic band adhesives

Malkoç¹,

Çörekçi²,

Botsali³

et al. 2010

The Angle Orthodontist

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) were prepared and the samples were extracted in 3 mL of Basal Medium Eagle with 10% newborn calf serum for 24 hours. The L929 cells were plated (25,000 cells/mL) in wells of 96-well dishes and maintained in a humidified incubator for 24 hours at 37uC, 5% CO 2 , and 95% air. After 24-hour incubation of the cells, the incubation medium was replaced by the immersed medium in which the samples were stored. Then L929 cells were incubated in contact with eluates for 24 hours. The cell mitochondrial activity was evaluated by the methyltetrazolium test. Twelve wells were used for each specimen, and methyltetrazolium tests were applied two times. The data were statistically analyzed using one-way analysis of variance and Tukey Honestly Significantly Different tests. Results:Results with L929 fibroblasts demonstrated that all freshly prepared resin-modified orthodontic band adhesive materials reduced vital cell numbers (P . .05), in comparison to the control group. Our data demonstrate that all materials showed significant cytotoxicity compared to the control group. Conclusions:The results indicate that all materials showed significant cytotoxicity compared to the control group, and further studies using different test methods are needed for all resin-modified orthodontic band adhesives. (Angle Orthod. 2010;80:890-895.)

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“…Whole resin composites, when placed into culture medium with cells, caused a variety of cytotoxic effects (Spangberg et al, 1973;Hensten-Pettersen and Helgeland, 1977;Tyas and Browne, 1977;Hanks et al, 1981;Imai and Nakamura, 1989;Kaga etal, 1989;Meryon and Brook, 1989). Jontell et al (1995) described the cytotoxic effects of several resins on lymphocytes and also noted stimulation of lymphocyte proliferation at low concentrations, which may be significant in possible immune responses.…”

Section: Indirect Evidence For Releasecytotoxic Responses In Vitromentioning

confidence: 99%

Bioavailability of Components of Resin-Based Materials Which Are Applied To Teeth

Hume

Gerzina

1996

Critical Reviews in Oral Biology & Medicine

148

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Chemical components of many materials used in dental practice can move into the local biophase, where they can have beneficial or adverse effects. The strongest indirect evidence that components of resin-based materials used in dentistry can move into the biophase are the many reports of allergic dermatitis in dental personnel. Direct measurement of component release has shown that triethylene glycol dimethacrylate (TEGDMA), hydroxyethyl methacrylate (HEMA), and, in the case of some orthodontic cements, bis-glycidyl methacrylate and benzoyl peroxide can move into an aqueous medium from a range of resin-based materials which are applied to teeth as part of oral care. In the case of resin composite restorations, HEMA and TEGDMA are available in microgram quantities via the salivary surface in the minutes and hours after clinical placement and via dentin and pulp in the hours and days after placement. Fortunately, moderate thickness of dentin protects pulp tissue against local toxicity. There are no data which suggest that systemic toxicity is a risk with any of these materials. There are some case reports of allergic responses to the monomers in patients, but the incidence of such responses appears at present to be much lower than that in dental personnel.

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Cytotoxic effects of dental cements on two cell culture systems

Cited by 96 publications

References 24 publications

Elution study of unreacted TEGDMA from bulk-fill composite (SDR™ Dentsply) using HPLC

Elution study of unreacted TEGDMA from bulk-fill composite (SDR™ Dentsply) using HPLC

Cytotoxic effects of resin-modified orthodontic band adhesives

Bioavailability of Components of Resin-Based Materials Which Are Applied To Teeth

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