Cytotoxic effects ofEryngium kotschyiandEryngium maritimumon Hep2, HepG2, Vero and U138 MG cell lines

Abstract

Context: Eryngium maritimum L. and the endemic Eryngium kotschyi Boiss. of the Apiaceae family are used for antiinflammatory, antivenom, antinociceptive and diuretic purposes in folk medicine in Turkey.Objective: This study investigated the cytotoxic effects of the plant extracts belonging to Eryngium L. genus on various cell lines. Materials and methods: Cytotoxic activites of the lyophilized aqueous aereal and root parts of the plant extracts on human hepatocellular carcinoma (HepG2), human laryngeal epidermoid carcinoma (Hep2), human glioma (U138-MG) and African green monkey kidney epithelial (Vero) cell lines at 8.33-266.62 mg/ml concentrations were analyzed by lactate dehydrogenase (LDH) leakage and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) cell viability assays. Results: Inhibitory concentration 50 (IC 50 ) values were found 5100 mg/ml in most cases varying around 16.33-125.66 mg/ml. IC 50 values for E. kostchyi and E. maritimum root parts on Hep2 cells (32.86 and 30.25 mg/ml, respectively), E. kotschyi aereal, E. maritimum aereal and root parts on HepG2 cells (31.75, 32.42 and 35.01 mg/ml, respectively) by MTT assay were found to be close to the US National Cancer Institute (NCI) recommendations (IC 50 530 mg/ml) to define the antivity aganist cancer cells. The lowest IC 50 values according to the LDH method were observed in Hep2 cells and the highest in U138-MG cells. Root parts were found to be more toxic than aereal parts for both plants in both methods in general. Discussion and conclusion: Both plant extracts exerted cytotoxic activity aganist Hep2 and HepG2 cells, with low IC 50 values defining their promising anticancer property according to NCI; however, further analysis are needed to confirm their activity.

Antiproliferative activity of Syzygium coriaceum, an endemic plant of Mauritius, with its UPLC-MS metabolite fingerprint: A mechanistic study

“…accordance with the US National Cancer Institute guidelines, extracts with IC 50 values below 30 μg/mL are considered potent candidates for further investigations with regards to their anticancer potential [35]. Consequently, it is of value to gauge the S. coriaceum effect on the replicative ability of the cancer cells, at doses equivalent to ½ X, X and 2X IC 50 values.…”

Edible halophytes of the Mediterranean basin: Potential candidates for novel food products

“…Eryngium maritimum is also an important halophyte, since leaves extracts exhibited strong antioxidant activity and antibacterial activity against Pseudomonas aeruginosa and Pseudomonas fluorescens (Meot-Duros, Le Floch, & Magné, 2008), while Yurdakök and Baydan (2013) reported that extracts from the aerial and root parts showed cytotoxic effects against human hepatocellular carcinoma (HepG2) and human laryngeal epidermoid carcinoma (Hep2) cells. In a recent study, Amessis-Ouchemoukh et al 2014observed that leaves extracts exhibited significant acetylcholinesterase inhibitory activity, while stems had a stronger metal chelating activity than leaves.…”

Nonhuman Primates

“…As with the baboon and rhesus macaque, genetic maps have been developed for C. a. sabaeus (Jasinska et al, 2007). The use of this cell line continues to be widespread in biomedical research Yurdakök and Baydan, 2013). Some of the many research areas include African trypanosomiasis infection (Ouwe-Missi-Oukem- Boyer et al, 2006), vaccinology (Martín et al, 2009), HIV/ AIDS (Hatziioannou and Evans, 2012), hypertension (Ervin and Palmour, 2003), neurological disease (Lemere et al, 2004;Harvey et al, 2000), psychology and social behavior (Bailey et al, 2007;Fairbanks et al, 2004), atherosclerosis and metabolic syndrome (Rayner et al, 2011;van Jaarsveld et al, 2002), and leishmania (Olobo et al, 2001).…”