Cytotoxic effects of infusions (tea) of Solidago microglossa DC. (Asteraceae) on the cell cycle of Allium cepa

Abstract

DC. (Asteraceae) no ciclo celular de Allium cepa". A espécie medicinal Solidago micrglossa DC., a qual é nativa da América do Sul, é usada como infusão (chá) para desordens estomacais na medicina popular. O objetivo desse estudo foi avaliar o potencial efeito citotóxico dessas infusões no ciclo celular de Allium cepa. Folhas in natura de três populações dessa espécie foram utilizadas no preparo das infusões em duas concentrações: 1,75 mg/mL e 14 mg/mL. Células de raízes de cebola (Allium cepa) foram usadas como sistema teste nos ensaios in vivo. As lâminas foram feitas através da técnica de esmagamento. As células foram analisadas em todos os ciclos celulares de A. cepa, totalizando 6000 células para cada grupo de bulbos. O índice mitótico (IM) foi calculado e a análise estatística foi realizada através do teste Qui-quadrado (χ 2 = 0,05). Os resultados mostraram que as infusões de S. microglossa na maior concentração (14 mg/mL) causou uma redução no IM comparado com os controles nas populações estudadas. Nas concentrações comumente usadas pelas pessoas houve um aumento significativo do IM em relação ao controle nas populações 1 e 3 e foi reduzido na população 2. Em conclusão, infusões de S. microglossa apresentam efeito antiproliferativo na maior concentração testada.Unitermos: Sistema teste vegetal, planta medicinal, citotóxico, Solidago microglossa, Asteraceae. ABSTRACT:The medicinal specie Solidago microglossa DC., which is native from South America, is used as infusion (tea) for stomach disorders in popular medicine. This study aimed to evaluate the potential cytotoxic effect of these infusions on the cell cycle of Allium cepa. In natura leaves of three populations of this species were used in preparing infusions at two concentrations: 1.75 mg/mL and 14 mg/mL. Onion (Allium cepa) root-tip cells were used as test system in in vivo assays. Slides were done through smashing technique. Cells were analyzed in all cell cycles of A. cepa, totalizing 6000 cells for each group of bulbs. The mitotic index (MI) was calculated, and the statistical analysis was carried out through the Chi-square test (χ 2 = 0.05). The results show that the infusions of S. microglossa at a highest concentration (14 mg/mL) caused a significant reduction in the MI compared with control in the studied populations. In concentrations commonly used by people there was a significant increase of MI in relation to controlled populations 1 and 3 and a decrease in population 2. In conclusion, infusions of S. microglossa presented antiproliferative effect at a highest concentration.

Evaluation of the genotoxicity of aqueous extracts of leaves and stems of Dolichandra unguis-cati (L.) L.G. Lohmann (Bignoniaceae) using the Allium cepa test

“…The concentrations (20g/L) (mitotic index=11) and (12.5g/L) (mitotic index=12) also inhibited mitosis with respect to the control by 26% and 20% respectively. This decrease in the mitotic index as the concentration of the extracts increases coincides with the results of other medicinal extracts, for example extracts of Psidium guajava, 7 of Solidago microglossa 14 and of Pterocaulon polystachyum. 15…”

Assessment of the antiproliferative and antigenotoxic activity and phytochemical screening of aqueous extracts of Sambucus australis Cham. & Schltdl. (ADOXACEAE)

“…The evaluation of the effect caused by infusions carried out with Solidago microglossa DC. leaves on the Allium cepa cell cycle, Bagatini et al (2009) observed that the highest concentration tested (14 mg/ml) caused a reduction in the mitotic index, thus demonstrating the antiproliferative effect of this species.…”

Chromatographic analysis and antiproliferative potential of aqueous extracts of Punica granatum fruit peels using the Allium cepa test

“…The same was observed in Artemisia verlotorum Lamotte by Souza et al (2010). Bagatini et al (2009), while studying Solidago microglossa D.C., found antiproliferative activity in the extract with the highest concentration compared to the negative control.…”

“…The same was observed in Artemisia verlotorum Lamotte by Souza et al (2010). Bagatini et al (2009), while studying Solidago microglossa D.C., found antiproliferative activity in the extract with the highest concentration compared to the negative control.The A. cepa test can be considered a bioindicator for monitoring the genotoxic potential of several chemical compounds. Chauan et al (1999) used meristematic cells of A. cepa to study the clastogenic potential of the pesticides cypermethrim and fenvalerate and obtained a good correlation with the test in mammals.…”