Cytotoxic effects of oosporein isolated from endophytic fungus Cochliobolus kusanoi

Alurappa, Ramesha; Mudili, Venkataramana; Nirmaladevi, D.; Gupta, Vijai Kumar; Siddaiah, Chandranayaka; Srinivas, Ch.

doi:10.3389/fmicb.2015.00870

Cited by 23 publications

(8 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The cytotoxicity of oosporein was evaluated toward A549 and presented the IC 50 value of 21 μM (Alurappa et al, 2014 ). The cytotoxic potential of oosporein in the MDCK cell line and its role in oxidative stress in vivo and in vitro have been previously reported by our research group (Ramesha et al, 2015 ).…”

Section: Cytotoxic Compounds From Endophytic Fungi Associated With Tesupporting

confidence: 60%

Endophytic Fungi—Alternative Sources of Cytotoxic Compounds: A Review

et al. 2018

Self Cite

View full text Add to dashboard Cite

Cancer is a major cause of death worldwide, with an increasing number of cases being reported annually. The elevated rate of mortality necessitates a global challenge to explore newer sources of anticancer drugs. Recent advancements in cancer treatment involve the discovery and development of new and improved chemotherapeutics derived from natural or synthetic sources. Natural sources offer the potential of finding new structural classes with unique bioactivities for cancer therapy. Endophytic fungi represent a rich source of bioactive metabolites that can be manipulated to produce desirable novel analogs for chemotherapy. This review offers a current and integrative account of clinically used anticancer drugs such as taxol, podophyllotoxin, camptothecin, and vinca alkaloids in terms of their mechanism of action, isolation from endophytic fungi and their characterization, yield obtained, and fungal strain improvement strategies. It also covers recent literature on endophytic fungal metabolites from terrestrial, mangrove, and marine sources as potential anticancer agents and emphasizes the findings for cytotoxic bioactive compounds tested against specific cancer cell lines.

show abstract

Section: Cytotoxic Compounds From Endophytic Fungi Associated With Tesupporting

confidence: 60%

Endophytic Fungi—Alternative Sources of Cytotoxic Compounds: A Review

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Oosporein produced by B. caledonica has immunosuppressive properties and contributes to greater and faster mortality of insects (Mc Namara et al 2019). Oosporein from Cochliobolus kusanoi exerts cytotoxic effects on Madin-Darby canine kidney (MDCK) and mouse macrophage (RAW 264.7), which is mediated by ROS through oxidative stress that increases lipid peroxidation and glutathione (GSH) level (Ramesha et al 2015).…”

Section: Secondary Metabolites: Role In Infection and Possible Applicmentioning

confidence: 99%

Entomopathogenic fungi: unconventional applications

Litwin

Nowak

Różalska

2020

Rev Environ Sci Biotechnol

177

View full text Add to dashboard Cite

Entomopathogenic fungi (EPF) are microorganisms that cause fatal diseases of arthropods. The infection process involves several stages that consist of direct contact of the fungus with the surface of the cuticle of the attacked insect. The factors that determine the effectiveness of the infection process include lytic enzymes, secondary metabolites, and adhesins produced by EPF. Because of their high insecticidal effectiveness, these fungi are commonly used as biopesticides in organic farming. As the environment and farmlands are contaminated with many compounds of anthropogenic origin (e.g., pesticides), the effects of these toxic compounds on EPF and the mechanisms that affect their survival in such a toxic environment have been studied in recent years. This review presents information on the capacity of EPF to remove toxic contaminants, including alkylphenols, organotin compounds, synthetic estrogens, pesticides and hydrocarbons. Moreover, these fungi produce numerous secondary metabolites that can be potentially used in medicine or as antimicrobial agents. Despite their huge potential in biocontrol processes, the use of EPF has been underestimated due to a lack of knowledge on their abilities. In our work, we have presented the available data on the possibilities of the additional and unconventional use of these microorganisms.

show abstract

“…However, the confirmation of oosporein production in C. cicadae indicates that this fungus could potentially cause avian gout or mortality in chickens and birds [ 53 , 54 ]. Previous in vivo and in vitro assays also indicated that oosporein could cause cytotoxicity in the canine kidney and lead to tissue damages in mice in a dose-dependent manner by inducing oxidative stress [ 55 ]. In addition, our transcriptomic and metabolomic data indicated that the oxalic acid (OS) biosynthetic pathway was up-regulated during fungal fruiting, and OS was more highly accumulated in the fruiting-body samples when compared to the liquid cultures.…”

Section: Discussionmentioning

confidence: 99%

Omics data reveal the unusual asexual-fruiting nature and secondary metabolic potentials of the medicinal fungus Cordyceps cicadae

Luo

Cen

et al. 2017

BMC Genomics

View full text Add to dashboard Cite

BackgroundAscomycete Cordyceps species have been using as valued traditional Chinese medicines. Particularly, the fruiting bodies of Cordyceps cicadae (syn. Isaria cicadae) have long been utilized for the treatment of chronic kidney disease. However, the genetics and bioactive chemicals in this fungus have been largely unexplored.ResultsIn this study, we performed comprehensive omics analyses of C. cicadae, and found that, in contrast to other Cordyceps fungi, C. cicadae produces asexual fruiting bodies with the production of conidial spores instead of the meiotic ascospores. Genome sequencing and comparative genomic analysis indicate that the protein families encoded by C. cicadae are typical of entomopathogenic fungi, including the expansion of proteases and chitinases for targeting insect hosts. Interestingly, we found that the MAT1-2 mating-type locus of the sequenced strain contains an abnormally truncated MAT1-1-1 gene. Gene deletions revealed that asexual fruiting of C. cicadae is independent of the MAT locus control. RNA-seq transcriptome data also indicate that, compared to growth in a liquid culture, the putative genes involved in mating and meiosis processes were not up-regulated during fungal fruiting, further supporting asexual reproduction in this fungus. The genome of C. cicadae encodes an array of conservative and divergent gene clusters for secondary metabolisms. Based on our analysis, the production of known carcinogenic metabolites by this fungus could be potentially precluded. However, the confirmed production of oosporein raises health concerns about the frequent consumption of fungal fruiting bodies.ConclusionsThe results of this study expand our knowledge of fungal genetics that asexual fruiting can occur independent of the MAT locus control. The obtained genomic and metabolomic data will benefit future investigations of this fungus for medicinal uses.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-4060-4) contains supplementary material, which is available to authorized users.

show abstract

Cytotoxic effects of oosporein isolated from endophytic fungus Cochliobolus kusanoi

Cited by 23 publications

References 41 publications

Endophytic Fungi—Alternative Sources of Cytotoxic Compounds: A Review

Endophytic Fungi—Alternative Sources of Cytotoxic Compounds: A Review

Entomopathogenic fungi: unconventional applications

Omics data reveal the unusual asexual-fruiting nature and secondary metabolic potentials of the medicinal fungus Cordyceps cicadae

Contact Info

Product

Resources

About