Cytotoxic ent-Abietane-type diterpenoids from the roots of Euphorbia ebracteolata

Han, Chunhui; Peng, Yulin; Wang, Yifei; Huo, Xiaokui; Zhang, Baojing; Li, Dawei; Leng, Aijing; Zhang, Hou-Li; Ma, Xiaochi; Wang, Chao

doi:10.1016/j.bioorg.2018.07.032

Cited by 24 publications

(13 citation statements)

References 20 publications

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“…These results indicated that TDEE was the anti-ascites active fraction of EER. Previous studies have shown that the diterpenoids from EER have inhibitory activities on tumor cells in vitro [5,23]. However, this study found that they have no effects on the tumor cell viability, cell cycle, or apoptosis of tumor cells.…”

Section: Discussioncontrasting

confidence: 68%

“…The root of EEH (EER) contains various terpenoids, which are considered to be effective components. Recent studies on terpenoids have been conducted to determine their anti-inflammatory, antiviral, anti-tuberculosis, and other effects in vitro [2][3][4][5]. However, basic research investigations on the traditional effects of EER in the treatment of abdominal distension and edema are limited.…”

Section: Introductionmentioning

confidence: 99%

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Anti-Malignant Ascites Effect of Total Diterpenoids from Euphorbiae ebracteolatae Radix Is Attributable to Alterations of Aquaporins via Inhibiting PKC Activity in the Kidney

et al. 2021

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This study evaluated the anti-ascites effect of total diterpenoids extracted from Euphorbiae Ebracteolatae Radix (TDEE) on malignant ascitic mice and elucidated its underlying mechanism. TDEE was extracted by dichloromethane and subjected to column chromatography. The purity of six diterpenoids isolated from TDEE was determined to be 77.18% by HPLC. TDEE (3 and 0.6 g raw herbs/kg, p.o.) reduced ascites and increased urine output. Meanwhile, analysis of tumor cell viability, cycle and apoptosis indicated that TDEE had no antitumor activity. In addition, the expression levels of aquaporins (AQPs) and the membrane translocation levels of protein kinase C (PKC) α and PKCβ in kidney and cells were measured. TDEE reduced the levels of AQP1–4, and inhibited PKCβ expression in membrane fraction. Four main diterpenoids, except compound 2, reduced AQP1 level in human kidney-2 cells. Compounds 4 and 5 inhibited AQP2–4 expression in murine inner medullary collecting duct cells. The diterpenoid-induced inhibition of AQP1–4 expression was blocked by phorbol-12-myristate-13-acetate (PMA; agonist of PKC). The diterpenoids from TDEE are the main anti-ascites components. The anti-ascites effect of diterpenoids may be associated with alterations in AQPs in the kidneys to promote diuresis. The inhibition of AQP1–4 expression by TDEE is related to the inhibition of PKCβ activation.

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Section: Discussioncontrasting

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Anti-Malignant Ascites Effect of Total Diterpenoids from Euphorbiae ebracteolatae Radix Is Attributable to Alterations of Aquaporins via Inhibiting PKC Activity in the Kidney

et al. 2021

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“…The cytotoxicity assay was carried out using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide method, as previously described. 15,16 5-Fluorouracil (Sigma, St. Louis, MO, United States) was used as a positive control, and the IC 50 values were calculated by Graphpad prism 7.0.…”

Section: Methodsmentioning

confidence: 99%

“…The cytotoxicity assay was carried out using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide method, as previously described. 15,16…”

Section: Biological Activitiesmentioning

confidence: 99%

Chemical Constituents and Their Activities From the Seeds of Ormosia hosiei

Qiu

Chen

et al. 2019

Natural Product Communications

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A new cytisine-like alkaloid, hosimonoal (1), with a fused monoterpene group, and 6 known compounds (2-6) were isolated from the seeds of Ormosia hosiei Hemsl. et Wils. The structure of the new compound was confirmed by high resolution electrospray ionization mass spectroscopy (HRESIMS), 1D and 2D nuclear magnetic resonance (NMR) spectroscopy, and electronic circular dichroism, and the others by 1H and 13C NMR spectroscopy and comparison with the literature data. In addition, compounds 1, 2, 3, and 6 showed moderate cytotoxic effects against A2780, A-549, HepG2, and MCF-7 cell lines with IC50 values ranging from 5.7 to 36.5 μM.

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“…Especially, diterpenoids as the major constituents of E. ebracteolata displayed a wide spectrum of bioactivities, including anti-tumor [8][9], anti-inflammatory [10], anti-HIV [11], and anti-tuberculosis [12]. In our previous work, 40 new and 45 known compounds were isolated from the ethanoic extracts of the roots of E. ebracteolata [12][13][14][15][16][17][18][19][20].…”

Section: Previous Studiesmentioning

confidence: 99%

Aromatic Rosane-type Diterpenoid with Lipase Inhibitory Effect from Euphorbia ebracteolata and Chemotaxonomic Significance of Diterpenoids

Zhou¹,

Wen²,

Huang³

et al. 2021

Rec.Nat.Prod.

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The phytochemical investigation has been performed for Euphorbia ebracteolata, the roots of which are usually used natural medicine in traditional Chinese medicine for the treatments of tuberculosis and bacterial infection. A diterpenoid has been obtained using silica gel and ODS column chromatography. Furthermore, on the basis of widely spectroscopic data analyses, including 1D-, 2D-NMR, HR-ESIMS and ECD, the isolated compound was determined to be a rosane type diterpenoid, which possessed a rare aromatic ring. The isolated diterpenoid as a new compound was named ebraphenol E (1). The bioactivity of isolated diterpenoid (1) has been evaluated and moderate inhibitory effect on lipase (IC50 = 12.5 μM) was observed. In combination with our previous phytochemical investigations about E. ebracteolata, the chemotaxonomic significance of diterpenoids was summarized for E. ebracteolata.

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Cytotoxic ent-Abietane-type diterpenoids from the roots of Euphorbia ebracteolata

Cited by 24 publications

References 20 publications

Anti-Malignant Ascites Effect of Total Diterpenoids from Euphorbiae ebracteolatae Radix Is Attributable to Alterations of Aquaporins via Inhibiting PKC Activity in the Kidney

Anti-Malignant Ascites Effect of Total Diterpenoids from Euphorbiae ebracteolatae Radix Is Attributable to Alterations of Aquaporins via Inhibiting PKC Activity in the Kidney

Chemical Constituents and Their Activities From the Seeds of Ormosia hosiei

Aromatic Rosane-type Diterpenoid with Lipase Inhibitory Effect from Euphorbia ebracteolata and Chemotaxonomic Significance of Diterpenoids

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