Cytotoxic, genotoxic and pro-inflammatory effects of zinc oxide nanoparticles in human nasal mucosa cells in vitro

Hackenberg, Stephan; Scherzed, Agmal; Technau, Antje; Kessler, M.; Froelich, Katrin; Ginzkey, Christian; Koehler, Christian; Burghartz, Marc; Hagen, Rudolf; Kleinsasser, Norbert

doi:10.1016/j.tiv.2011.01.003

Cited by 195 publications

(126 citation statements)

References 39 publications

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“…The result that ZnO particles displayed a steep decrease in viability above 10 μg/mL ZnO is consistent with the studies of Heng et al (24) and Hackenberg et al (25). The other study from Yang et al (23) on cytotoxic effects of four typical nanoparticles showed that zinc oxide induced much greater cytotoxicity than nonmetal nanoparticles.…”

Section: In Vitro Cytotoxicity Of Zno Particlessupporting

confidence: 90%

“…The other study from Yang et al (23) on cytotoxic effects of four typical nanoparticles showed that zinc oxide induced much greater cytotoxicity than nonmetal nanoparticles. The study also indicated that oxidative stress might be a key route in inducing the cytotoxicity of nanoparticles (24,25), and demonstrated in the end that particle composition probably played a primary role in the cytotoxic effects.…”

Section: In Vitro Cytotoxicity Of Zno Particlesmentioning

confidence: 81%

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Effect of Particle Size of Zinc Oxides on Cytotoxicity and Cell Permeability in Caco-2 Cells

Chang¹,

Choi²,

Ko³

et al. 2011

JFN

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The cell permeability and cytotoxic effects of different-sized zinc oxide (ZnO) particles were investigated using a human colorectal adenocarcinoma cell line called Caco-2. Morphological observation by scanning electron microscopy revealed that three zinc oxides with different mean particle sizes (ZnO-1, 20 nm; ZnO-2, 90～200 nm; ZnO-3, 1～5 μm) tended to aggregate, particularly in the case of ZnO-1. When cytotoxicities of all three sizes of zinc oxide particles were measured at concentration ranges of 1～1000 μg/mL, significant decreases in cell viability were observed at concentrations of 50 μg/mL and higher. Among the three zinc oxides, ZnO-1 showed the lowest viability at 50 μg/mL in Caco-2 cells, followed by ZnO-2 and ZnO-3. The permeate concentration of ZnO-1 from the apical to the basolateral side in the Caco-2 model system after four hours was about three-fold higher than that of either ZnO-2 or ZnO-3. These results demonstrated that ZnO-1, with a 20 nm mean particle size, had poorer viability and better permeability in Caco-2 cells than ZnO-2 and ZnO-3.

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Section: In Vitro Cytotoxicity Of Zno Particlessupporting

confidence: 90%

Section: In Vitro Cytotoxicity Of Zno Particlesmentioning

confidence: 81%

Effect of Particle Size of Zinc Oxides on Cytotoxicity and Cell Permeability in Caco-2 Cells

Chang¹,

Choi²,

Ko³

et al. 2011

JFN

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“…Nasal mucosa cells Cytotoxic, genotoxic and pro-inflammatory effects [77] Hepatocytes Inflammatory and genotoxic effects [56] Renal cells Cytotoxicity [78] Neurons Neurotoxic effect by disturbing the electrical activity of neuronal networks [7] Lung epithelial cells Cytotoxicity [79] Carbon black nanoparticles (CB-NPs) Lymphocytes Induction of chromosomal aberrations [80] Hepatocytes Hepatotoxicity [81] Neurons Dopaminergic neurons damage pathways [82] Lung epithelial cells Toxicity and inflammatory response. [83] Lymphocytes Cytotoxicity and genotoxicity [84] Silica nanoparticles (SiO 2 ) Fibroblast Cytotoxicity [25] Hepatocytes Genotoxicity, carcinogenicity, hepatotoxicity and inflammation [48,85] Renal cells Induction of oxidative stress and cytotoxicity [86] Neurons Neurotoxicity [7] Lung epithelial cells Genotoxicity, mutagenicity and carcinogenicity [87,88] Lymphocytes Induction of oxidative stress and reduction of immune capacity, Genotoxicity [27,80] Titanium dioxide nanoparticles (TiO 2 )…”

Section: Hepatocytesmentioning

confidence: 99%

Nanoparticle Technology as a Double-Edged Sword: Cytotoxic, Genotoxic and Epigenetic Effects on Living Cells

Jennifer¹,

Wnuk²

2013

JBNB

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Nanoparticles are considered as powerful tools in nanotechnological applications. Due to their unique physicochemical properties, their interactions with different biological systems have been shown. Nanomaterials have been successfully used as coating materials or treatment and diagnosis tools. Nevertheless, toxic effects of nanoparticles in vitro and in vivo have also been reported. Here, we summarize the current state of knowledge on exposure routes, cellular uptake and toxicological activities of the commonly used nanoparticles. In this context, we discuss the mechanisms of toxicity of nanoparticles involving perturbation of redox milieu homeostasis and cellular signaling pathways.

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“…Additionally, the potential repair of DNA fragmentation after 24 hr of regeneration was examined. Results of a prior study revealed significant DNA damage in human air-liquid interface nasal mucosa cell cultures after exposure to ZnO-NPs at concentrations of 10 lg/ml [Hackenberg et al, 2011]. Thus, sub-genotoxic ZnO-NP concentrations (<10 lg/ml) were chosen in the current study to investigate cumulative genotoxic effects.…”

Section: Introductionmentioning

confidence: 99%

Repetitive exposure to zinc oxide nanoparticles induces dna damage in human nasal mucosa mini organ cultures

Hackenberg

Zimmermann

Scherzed

et al. 2011

Environ and Mol Mutagen

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Data on the toxicological properties of zinc oxide nanoparticles (ZnO-NPs) is incomplete. ZnO-NPs may enter humans via inhalation or ingestion. The aim of the current study was to evaluate ZnO-NP-induced genotoxicity in three-dimensional (3D) mini organ cultures (MOCs) of human nasal mucosa following repeated exposure to ZnO-NP and regeneration. Nasal MOCs of 10 patients and ZnO-NPs were cultivated for one week and then characterized by electron microscopy. Nasal MOCs were partially covered by ciliated epithelium after one week of cultivation. ZnO-NPs were distributed to the cytoplasm and the nucleus. MOCs were exposed once, twice, or three times to 0.1 or 5 μg/ml of ZnO-NPs for 1 hr per exposure and were then evaluated for cytotoxicity and genotoxicity. MOCs were cultivated for 24 hr after the triple ZnO-NP exposure to allow for regeneration. ZnO-NP exposure did not result in significant cytotoxicity or apoptosis, as determined by trypan blue exclusion and caspase-3 activity, respectively. A significant increase in DNA damage was detected following repetitive exposure compared to single exposure to ZnO-NPs at 5 μg/ml, but not 0.1 μg/ml ZnO-NPs. At both concentrations of ZnO-NP, DNA fragmentation increased after 24 hr of regeneration. In contrast, DNA damage which was induced by the positive control, methyl methanesulfonate, was significantly reduced after 24-hr regeneration. Thus, our results suggest that repetitive exposure to low concentrations of ZnO-NPs results in persistent or ongoing DNA damage.

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Cytotoxic, genotoxic and pro-inflammatory effects of zinc oxide nanoparticles in human nasal mucosa cells in vitro

Cited by 195 publications

References 39 publications

Effect of Particle Size of Zinc Oxides on Cytotoxicity and Cell Permeability in Caco-2 Cells

Effect of Particle Size of Zinc Oxides on Cytotoxicity and Cell Permeability in Caco-2 Cells

Nanoparticle Technology as a Double-Edged Sword: Cytotoxic, Genotoxic and Epigenetic Effects on Living Cells

Repetitive exposure to zinc oxide nanoparticles induces dna damage in human nasal mucosa mini organ cultures

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