Cytotoxic HL‐A Antibodies: Immunoglobulin Classification

Ahrons, S.; Glavind-Kristensen, S.

doi:10.1111/j.1399-0039.1971.tb00089.x

Cited by 34 publications

(3 citation statements)

References 13 publications

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“…It can be seen that in only one case is there evidence of inhibition by a 'supernatant' fraction and this is very slight. All the serum 'supernatant' fractions tested in these experiments were also tested in a cytotoxic assay and were shown to have retained specific cytotoxic antibody, presumably 7 s. Most specific antibodies in multiparous sera have been found to be 7 s (Ahrons & Glavind-Kristensen 1971). In some experiments complement was added to cells which had been preincubated with serum 'supernatants' and washed ready for rosette formation, t o test for bound cytotoxic antibody.…”

Section: Icesultsmentioning

confidence: 99%

Relationship between the HLA Associated Specificities 4a and 4b and the Lymphocyte C3 Receptor

Frew

Bright

1977

Tissue Antigens

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Contrary to previous reports, we have obtained no evidence that 4a and 4b antisera specifically inhibit C3 rosette formation by human lymphocytes. Pretreatment of lymphocytes with various whole 4a and 4b antisera resulted in partial inhibition which was often nonspecific. Ultracentrifugation of the sera to remove immune complexes removed C3 rosette inhibitory activity although specific cytotoxic activity remained. It is probable that immune complexes in antisera will have fixed C3 which will have been converted to C3d and so be able to block the C3d receptor. This is the receptor mainly measured in these and in the previous experiments.

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Section: Icesultsmentioning

confidence: 99%

Relationship between the HLA Associated Specificities 4a and 4b and the Lymphocyte C3 Receptor

Frew

Bright

1977

Tissue Antigens

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“…Since in preliminary experiments IgC prepared by ion exchange chromatography of serum CMD shows only peak A of cytotoxic activity after IEF, it is tempting to speculate that IgM and/or highly charged IgG molecules (known to be relatively depleted by ion exchange chromatography) are important in the crossreactive activity observed in CMD and perhaps important in cross-reactivity in other anti-HL-A sera. IgM anti-HL-A antibodies have been reported (Stocker et al 1969, Ahrons & Glavind-Kristensen 1971, Colombani et al 1973) although most reports emphasize that the activity is predominantly IgG (Metzgar & Seigler 1967, Williams et al 1971 Glavind-Kristensen 197 1). However, in previously reported work ) on focused fractions of BC p-B, peak C, not peak A, was depleted in cross-reactivity suggesting that more than one mechanism may be involved.…”

Section: Disc Us F I Onmentioning

confidence: 99%

Isoelectric Focusing of an Anti‐HL‐A2 Antiserum Cross‐Reacting with W28

et al. 1976

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The cytotoxic activity of an anti-HL-A2 antiserum (1-oss-reactive with W28 appeared in three distinct peaks after the serum was subjected t o isoelectric focusing. The pH at which the peaks formed was reproducible using pH 5-8 or pH 3..5 10 gradients. Anti-HLA2 activity occurred in each peak, but the cross-reactive anti-W28 cytotoxicity appeared to be largely confined t o the second and third peaks. The first peak was exclusively Ig<;, the second contained Ig(; and IgM, and the third contained IgC, IgA, and IgM.Received for fiiLblzcrrtzon I Juls. riccuplrd 12 Orfobrr 197.5

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“…[20][21][22][23] Patients with primary RM have a prevalence of HLA antibodies of maximum 10%; 24,25 patients with secondary RM on the other hand may have a high prevalence of HLA antibodies. 26 Steinborn et al 27 found an association between an increased prevalence of HLA antibodies and placental abruption.…”

Section: Hla Antibodiesmentioning

confidence: 99%

Anti‐HY Responses in Pregnancy Disorders

Christiansen¹,

Steffensen²,

Nielsen³

2011

American J Rep Immunol

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Problem Cellular and humoral immune responses against male-specific minor histocompatibility (HY) antigens are important in the pathogenesis of graft-versus-host reactions and can be detected in women who have previously given birth to a boy. However, the importance of these responses for pregnancy outcome is unclear. Method of study Review of the current knowledge about the impact of anti-HY immunity on pregnancy outcome in terms of risk of miscarriage, placental abrup-tion and low birth weight. Results Women with secondary recurrent miscarriage (RM) more often have given birth to a boy compared with a girl prior to a series of miscarriages (P < 0.0001) and a firstborn boy seems significantly to reduce the chance of a subsequent live in these patients (P = 0.0003). Human leu-kocyte antigen (HLA) class II alleles known to restrict CD4 T-cell-mediated anti-HY responses were investigated among 358 patients with secondary RM and 203 of their children born prior to the miscarriages. The chance of a subsequent live birth in secondary RM patients with firstborn boys compared with firstborn girls was significantly lower in women with HY-restricting HLA class II alleles [OR: 0.17 (0.1-0.4), P = 0.0001]. In patients without these alleles, the chance of live birth was similar in those with firstborn boys and girls, respectively. In early pregnancy, both antibodies against HLA and HY antigens were found with increased prevalence in secondary patients with RM and in particular in those with a firstborn boy compared with controls (P = 0.005). The presence of these antibodies was associated with a low subsequent live birth rate, and the presence of HY antibodies was associated with a low male ⁄ female ratio (12% boys versus 88% girls) in subsequent live births (P = 0.03). Register-based population studies suggested that births of boys also are associated with subsequent adverse obstetric outcomes also in the background population. Conclusion Findings strongly indicate that aberrant maternal immune reactions against fetal HY antigens playing a role in secondary RM and other pregnancy complications. We propose pathogenetic pathways for these conditions that in our view best explain the findings.

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Cytotoxic HL‐A Antibodies: Immunoglobulin Classification

Cited by 34 publications

References 13 publications

Relationship between the HLA Associated Specificities 4a and 4b and the Lymphocyte C3 Receptor

Relationship between the HLA Associated Specificities 4a and 4b and the Lymphocyte C3 Receptor

Isoelectric Focusing of an Anti‐HL‐A2 Antiserum Cross‐Reacting with W28

Anti‐HY Responses in Pregnancy Disorders

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