Cytotoxic properties of the nitrosyl iron complex with phenylthiyl

Stupina, Tatyana S.; Parkhomenko, I. I.; Balalaeva, Irina V.; Kostyuk, G. V.; Санина, Н. А.; Терентьев, А. А.

doi:10.1007/s11172-011-0221-9

Cited by 17 publications

(8 citation statements)

References 22 publications

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“…The complex inhibited viability of HeLa cells with IC50 corresponding to the IC50 range found earlier for other cell lines. The IC50 of 2-AmPh is similar to that for HeLa cells of the structurally similar Ph complex [57]. Based on the results of MTT assay for 2-AmPh and 2-aminothiophenol (Figure 2A), it can be suggested that the toxicity of 2-AmPh might be partially accounted for by the presence of 2-aminothiophenyl moieties.…”

Section: Discussionsupporting

confidence: 54%

“…Cells were plated and treated as described above in the "Flow Cytometry" section. In some experiments NICs containing cysteamine and phenylthiyl ligands, CysAm and Ph complexes, respectively, were used at IC50 doses that are 3.5 µM for CysAm [56] and 25 µM for Ph [57]. After exposure to the studied compounds for 6 h, cells were washed with PBS and lysed on ice for 15 min in the buffer containing 10 mM HEPES (pH 7.9), 1.5 mM MgCl 2 , 10 mM KCl, 0.1% NP-40, 1 mM DTT, 1 mM PMSF, and protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA).…”

Section: Preparation Of Nuclear Extracts and Immunoblottingmentioning

confidence: 99%

“…After exposure to 2-AmPh the nuclear p65 level decreased substantially compared to the vehicle control ( Figure 3A). The effect of 2-AmPh was compared to that exerted by NICs containing cysteamine and phenylthiyl ligands (CysAm [56] and Ph [57] complexes, respectively). All complexes were used at corresponding IC50 doses.…”

Section: Effects Of 2-amph On Nf-κb Functionsmentioning

confidence: 99%

“…Ph decreased p65 nuclear content to the extent similar to that observed under 2-AmPh exposure, while CysAm had a weaker effect on nuclear p65. Ph and CysAm were shown earlier to cause cell death and affect nuclear levels of p50 subunit of NF-κB [56,57]. It is interesting that CysAm exerted weaker effect on nuclear p50 compared to Ph.…”

Section: Effects Of 2-amph On Nf-κb Functionsmentioning

confidence: 99%

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NO-Donor Nitrosyl Iron Complex with 2-Aminophenolyl Ligand Induces Apoptosis and Inhibits NF-κB Function in HeLa Cells

et al. 2018

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NO donating iron nitrosyl complex with 2-aminothiophenyl ligand (2-AmPh complex) was studied for its ability to cause cell death and affect nuclear factor kappa B (NF-κB) signaling. The complex inhibited viability of HeLa cells and induced cell death that was accompanied by loss of mitochondrial membrane potential and characteristic for apoptosis phosphatidylserine externalization. At IC50, 2-AmPh caused decrease in nuclear content of NF-κB p65 polypeptide and mRNA expression of NF-κB target genes encoding interleukin-8 and anti-apoptotic protein BIRC3. mRNA levels of interleukin-6 and anti-apoptotic protein BIRC2 encoding genes were not affected. Our data demonstrate that NO donating iron nitrosyl complex 2-AmPh can inhibit tumor cell viability and induce apoptosis that is preceded by impairment of NF-κB function and suppression of a subset of NF-κB target genes.

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Section: Discussionsupporting

confidence: 54%

Section: Preparation Of Nuclear Extracts and Immunoblottingmentioning

confidence: 99%

Section: Effects Of 2-amph On Nf-κb Functionsmentioning

confidence: 99%

Section: Effects Of 2-amph On Nf-κb Functionsmentioning

confidence: 99%

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NO-Donor Nitrosyl Iron Complex with 2-Aminophenolyl Ligand Induces Apoptosis and Inhibits NF-κB Function in HeLa Cells

et al. 2018

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“…It is known that the iron sulfonitrosyl complexes, being intermediates in the formation and decomposition of natural thiols cat alyzed by cellular iron, stimulate vasodilation [5][6][7], exhibit antitumor and antimetastatic activity , induce apoptosis of human tumor cells of different genesis [12][13][14], etc.…”

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confidence: 99%

Synthesis, structure, and NO-donor activity of bis(5-nitropyridine-2-thiolato)tetranitrosyliron

et al. 2012

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A new method is developed for the synthesis of the binuclear iron tetranitrosyl complex with 5 nitropyridine 2 thiolate, [Fe 2 (SC 5 H 3 N 2 O 2 ) 2 (NO) 4 ] (I), from 2,2' dithiobis(5 nitropyridine) and hydra zine hydrate in an aqueous alcohol solution followed by the replacement of the thiosulfate ligands in an aque ous solution of salt Na 2 [Fe 2 (S 2 O 3 ) 2 (NO) 4 ] ⋅ 4H 2 O by functional 5 nitropyridine 2 thiolates. It is established by the X ray diffraction method that the complex has the structure of the μ SR type and crystallizes as two polymorphs. The Moessbauer spectral parameters at 293 K (quadrupole splitting ΔE Q = 1.243(1) mm/s, iso meric shift δ Fe = 0.095(1) mm/s, and absorption line width Γ = 0.263(1) mm/s) for studied compound I are similar to the isoelectronic complexes with thiophenoxide, pyridyl 2 thiolate, and pyrimidyl 2 thiolate. According to the electrochemical analysis data, complex I is a more efficient donor of NO in hydrolytic decomposition in protic media with NO formation than its isostructural analog with the unsubstituted pyridylthiolate ligand. The maximum amount of NO generated by complex I in a 1% aqueous solution of dimethyl sulfoxide at T = 25°C and pH 7.0 is 7.4 nmoles and increases twofold with an increase in the acidity of the medium (to pH 6.5) or temperature (37°C).

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Bis(4-nitrobenzenethiolato)tetranitrosyldiiron: synthesis, structure, and pharmacological activity of a new nitric oxide (NO) donor

et al. 2017

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Cytotoxic properties of the nitrosyl iron complex with phenylthiyl

Cited by 17 publications

References 22 publications

NO-Donor Nitrosyl Iron Complex with 2-Aminophenolyl Ligand Induces Apoptosis and Inhibits NF-κB Function in HeLa Cells

NO-Donor Nitrosyl Iron Complex with 2-Aminophenolyl Ligand Induces Apoptosis and Inhibits NF-κB Function in HeLa Cells

Synthesis, structure, and NO-donor activity of bis(5-nitropyridine-2-thiolato)tetranitrosyliron

Bis(4-nitrobenzenethiolato)tetranitrosyldiiron: synthesis, structure, and pharmacological activity of a new nitric oxide (NO) donor

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