Cytotoxic response of breast cancer cell lines, MCF 7 and T 47 D to triphala and its modification by antioxidants

Sandhya, Tera; Mishra, Kaushala Prasad

doi:10.1016/j.canlet.2005.07.013

Cited by 74 publications

(44 citation statements)

References 35 publications

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“…As a result, the sensitivity of tumor cells to anticancer drugs was increased [32,33] . This study agreed with the study of Sandhya and Mishra [34] which showed the cytotoxic response of human breast cancer cell lines to Triphala, which contained T. bellerica, P. emblica and T. chebula extracts. It might be possible that T. bellerica and P. emblica extracts could induce ROS in the induction of apoptosis.…”

Section: Discussionsupporting

confidence: 92%

“…Plant derived polyphenols, including tannins and gallic acid, were reported to be the main constituents in P. emblica and T. bellerica [13,30] . Marienfeld et al [31] reported that tannic acid (TA) could inhibit the malignant cholangiocyte growth both in vitro and in vivo and also enhance sensitivity of Mz-ChA-1 cholangiocarcinoma cells to camptothecin cytotoxicity which might involve an effect on xenobiotic metabolism.…”

Section: Discussionmentioning

confidence: 99%

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Synergistic growth inhibitory effects of Phyllanthus emblica and Terminalia bellerica extracts with conventional cytotoxic agents: Doxorubicin and cisplatin against human hepatocellular carcinoma and lung cancer cells

Pinmai

Chunlaratthanabhorn²,

Ngamkitidechakul³

et al. 2008

WJG

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AIM:To examine the growth inhibitory effects of Phyllanthus emblica (P. emblica ) and Terminalia bellerica (T. bellerica ) extracts on human hepatocellular carcinoma (HepG2), and lung carcinoma (A549) cells and their synergistic effect with doxorubicin or cisplatin. METHODS:HepG2 and A549 cells were treated with P. emblica and T. bellerica extracts either alone or in combination with doxorubicin or cisplatin and effects on cell growth were determined using the sulforhodamine B (SRB) assay. The isobologram and combination index (CI) method of Chou-Talalay were used to evaluate interactions between plant extracts and drugs. R E S U LT S : P. e m b l i c a a n d T. b e l l e r i c a e x t r a c t s demonstrated growth inhibitory activity, with a certain degree of selectivity against the two cancer cell lines tested. Synergistic effects (CI < 1) for P. emblica / doxorubicin or cisplatin at different dose levels were demonstrated in A549 and HepG2 cells. The T. bellerica / cisplatin or doxorubicin also showed synergistic effects in A549 and HepG2 cells. In some instances, the combinations resulted in antagonistic effects. The dose reduction level was different and specific to each combination and cell line. CONCLUSION:The growth inhibitory activity of doxorubicin or cisplatin, as a single agent, may be modified by combinations of P. emblica or T. bellerica extracts and be synergistically enhanced in some cases. Depending on the combination ratio, the doses for each drug for a given degree of effect in the combination may be reduced. The mechanisms involved in this interaction between chemotherapeutic drugs and plant extracts remain unclear and should be further evaluated.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Synergistic growth inhibitory effects of Phyllanthus emblica and Terminalia bellerica extracts with conventional cytotoxic agents: Doxorubicin and cisplatin against human hepatocellular carcinoma and lung cancer cells

Pinmai

Chunlaratthanabhorn²,

Ngamkitidechakul³

et al. 2008

WJG

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“…Cell culture and harvesting 20 Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% inactivated fetal bovine serum (FBS) was used for culturing the MCF-7 (Human breast carcinoma) cell lines. MCF-7 (Human Breast carcinoma) cell line was procured from National Centre for Cell Sciences (NCCS), Pune, India.…”

Section: Preparation Of Rhizome Extractmentioning

confidence: 99%

“…21 MTT assay was used to assess the cytotoxicity based on the reduction of MTT by mitochondrial dehydrogenase enzyme of the viable cells to purple formazon product as described by Mosmann, (1983). 20) Briefly, cells were diluted in the growth medium and seeded in 96 well plates at a concentration of 1×10 5 cells/well. After 12 hours, the growth medium was replaced with exposure medium (DMEM without FBS) containing the different concentrations (1000µg/ml, 500µg/ml, 250µg/ml, 125µg/ml, 62.5µg/ml) of rhizome extracts [Combined rhizomes of Alpinia calcarata and Alpinia speciosa (RR2282) and rhizome synthesized gold nanoparticles (RR2283)].…”

Section: Preparation Of Rhizome Extractmentioning

confidence: 99%

Investigating the Cytotoxic Effect of Combined Rhizome Extract (Alpinia Calcarata and Alpinia Speciosa) and Gold Nanoparticle in the Breast Cancer McF-7 Cell Lines

L¹,

Suja²

2017

Int. J. Res. Ayurveda Pharm.

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This study was carried out to investigate the cytotoxicity of human breast carcinoma cell lines (MCF-7) using Alpinia calcarata and Alpinia speciosa (Family: Zingiberaceae) rhizomes and rhizome extracted gold nanoparticles (green synthesis). Cells were cultured in DMEM medium and treated with rhizomes (RR2282) and gold nanoparticles (RR2283) for consecutive days. Cell proliferation assay, DNA fragmentation assay and LDH leakage assays were performed to evaluate and investigate the cytotoxicity in MCF-7 cells. It can be seen that inhibition of cell proliferation induced by the drug was dependant on concentration of plant extracts and gold nano particles. At the highest concentration of RR2282 tested (1000µg/ml), the proliferation of MCF-7 decreased by 90.69±2.0% after 12h. On the other hand, the proliferation of MCF-7 decreased by 77.05±1.5% at the highest concentration (1000µg/ml) of RR2283 tested. When the concentration of RR2282 and RR2283 test samples were decreased, cell cytotoxicity percentage was found decreased. LDH leakage assay showed RR2282 and RR2283 were capable of killing MCF-7 cells more effectively by increasing the cell permeability leading to increased cell death. Electrophoretic analysis of DNA extracted from untreated and treated cells showed significant differences in DNA fragmentation. The DNA from untreated cells was not fragmented, but all other samples resulted in DNA fragmentation. The study concluded that the extracts of Alpinia spp could be considered as a promising chemotherapeutic agent in breast cancer treatment. Gold nanoparticles anticipated that nanoparticle-mediated targeted delivery of drugs might significantly reduce the dosage of anti-cancer drugs with better specificity, enhanced efficacy and lower toxicities.

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“…Apart from this, triphala has been shown to possess significant antioxidant (Naik et al, 2005;Mahesh et al, 2009;Hazra et al, 2010) and nitric oxide scavenging activity (Jagetia et al, 2004). In addition, the anti-cancer (Deep et al, 2005;Sandhya and Mishra, 2006;Shi et al, 2008), immunomodulatory (Srikumar et al, 2005) and anti-inflammatory (Rasool and Sabina, 2007) activity of triphala directly targets the common health problems associated with aging. The selective cytotoxic and apoptotic activity of triphala on cancer cells while sparing the adjacent normal cells (Shi et al, 2008) further strengthens its compelling role as a dietary supplement to promote longevity.…”

Section: Signaling Molecules That Mediate the Biological Effects Of Hmentioning

confidence: 99%

Herbal Antioxidants as Rejuvenators in Alternative Medicine

Jamesdaniel¹,

Samson²

2011

Phytochemicals - Bioactivities and Impact on Health

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Cytotoxic response of breast cancer cell lines, MCF 7 and T 47 D to triphala and its modification by antioxidants

Cited by 74 publications

References 35 publications

Synergistic growth inhibitory effects of Phyllanthus emblica and Terminalia bellerica extracts with conventional cytotoxic agents: Doxorubicin and cisplatin against human hepatocellular carcinoma and lung cancer cells

Synergistic growth inhibitory effects of Phyllanthus emblica and Terminalia bellerica extracts with conventional cytotoxic agents: Doxorubicin and cisplatin against human hepatocellular carcinoma and lung cancer cells

Investigating the Cytotoxic Effect of Combined Rhizome Extract (Alpinia Calcarata and Alpinia Speciosa) and Gold Nanoparticle in the Breast Cancer McF-7 Cell Lines

Herbal Antioxidants as Rejuvenators in Alternative Medicine

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