Cytotoxic T lymphocytes directed against HLA-Bw35-linked target determinants show differences in sensitivity toward antibiotics during sensitization period

Recently we could demonstrate that antibiotics can interact with polymorphic membrane determinants. As a result, these determinants can no longer react with their corresponding antisera (Claas et al. 1981, 1982). In the present experiments it was found that allogeneic cytotoxic T cells (CTLs), which were cultured in the presence of antibiotics, induced significantly stronger lysis against the specific target than CTLs which were cultured in the absence of antibiotics. In some cases the CML assay was only positive according to the European Workshop criteria when penicillin was added to the cultures, whereas the assay was negative in the absence of penicillin. On the other hand when CTLs were induced in an MLC culture without penicillin, lysis was higher against the target cell in the absence of penicillin than in the presence of penicillin. By adding penicillin to the effector phase, inhibition of lysis was observed. No difference in 3H-thymidine-incorporation was observed between MLC cultures with and without antibiotics. We conclude that antibiotics in the culture medium can influence the outcome of CML experiments.

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“…Preliminary data show that C'I'Ls against some HLA-linked target structures arc influ-cnced by penicillin and others are not (Horai et al 1982).…”

Section: Cw3 Drw9mentioning

confidence: 96%

The outcome of cell mediated lympholysis is influenced by the antibiotics in the culture medium

et al. 1982

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“…Regarding CD8 + T cell effector function, penicillin and streptomycin were shown to accelerate target cell lysis by CTLs ( Horai et al, 1982 ) and sulfamethoxazole-hydroxylamine was found to promote the transcription of mitochondrial iron transporters that are important for CTL-mediated cytotoxicity ( Reinhart et al, 2018 ).…”

Section: Various Effects Of Antibiotics On Proliferation Apoptosis An...mentioning

confidence: 99%

Pleiotropic effects of antibiotics on T cell metabolism and T cell-mediated immunity

et al. 2022

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T cells orchestrate adaptive and innate immune responses against pathogens and transformed cells. However, T cells are also the main adaptive effector cells that mediate allergic and autoimmune reactions. Within the last few years, it has become abundantly clear that activation, differentiation, effector function, and environmental adaptation of T cells is closely linked to their energy metabolism. Beyond the provision of energy equivalents, metabolic pathways in T cells generate building blocks required for clonal expansion. Furthermore, metabolic intermediates directly serve as a source for epigenetic gene regulation by histone and DNA modification mechanisms. To date, several antibiotics were demonstrated to modulate the metabolism of T cells especially by altering mitochondrial function. Here, we set out to systematically review current evidence about how beta-lactam antibiotics, macrolides, fluoroquinolones, tetracyclines, oxazolidinones, nitroimidazoles, and amphenicols alter the metabolism and effector functions of CD4+ T helper cell populations and CD8+ T cells in vitro and in vivo. Based on this evidence, we have developed an overview on how the use of these antibiotics may be beneficial or detrimental in T cell-mediated physiological and pathogenic immune responses, such as allergic and autoimmune diseases, by altering the metabolism of different T cell populations.

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Interaction of Penicillin with HLA-A and -B antigens

Claas¹,

Nieuwkoop²,

Berge³

et al. 1982

Human Immunology

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Cytotoxic T lymphocytes directed against HLA-Bw35-linked target determinants show differences in sensitivity toward antibiotics during sensitization period

Cited by 4 publications

References 9 publications

The outcome of cell mediated lympholysis is influenced by the antibiotics in the culture medium

The outcome of cell mediated lympholysis is influenced by the antibiotics in the culture medium

Pleiotropic effects of antibiotics on T cell metabolism and T cell-mediated immunity

Interaction of Penicillin with HLA-A and -B antigens

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