Cytotoxic T Lymphocytes Elicited by Dendritic Cell-Targeted Delivery of Human Papillomavirus Type-16 E6/E7 Fusion Gene Exert Lethal Effects on CaSki Cells

Wu, Xiangbing; Liu, Xing; Jiao, Qing-fang; Fu, Shao-yue; Bu, Youquan; Song, Fangzhou; Yi, Faping

doi:10.7314/apjcp.2014.15.6.2447

Cited by 11 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies reported pre-clinical and clinical trials based on dendritic cell vaccines for cervical cancer and HNSCC [ 75 – 77 ]. DCs are very cytotoxic towards HPV16 E6 and E7 proteins expressing cells [ 78 ], thus autologous DCs are generally pulsed with these antigens (E6, E7 or both peptides or proteins), with ex vivo altered tumor cells [ 53 , 75 , 76 ] or with transfected DNA to be activated [ 53 ].…”

Section: Dendritic Cells and Immune Checkpoint Moleculesmentioning

confidence: 99%

Activities of stromal and immune cells in HPV-related cancers

Júnior

Melo

Barros

et al. 2018

J Exp Clin Cancer Res

View full text Add to dashboard Cite

The immune system is composed of immune as well as non-immune cells. As this system is a well-established component of human papillomavirus- (HPV)-related carcinogenesis, high risk human papillomavirus (hrHPV) prevents its routes and mechanisms in order to cause the persistence of infection. Among these mechanisms are those originated from stromal cells, which include the cancer-associated fibroblasts (CAFs), the myeloid-derived suppressor cells (MDSCs) and the host infected cells themselves, i.e. the keratinocytes. These types of cells play central role since they modulate immune cells activities to create a prosperous milieu for cancer development, and the knowledge how such interactions occur are essential for prognostic assessment and development of preventive and therapeutic approaches. Nevertheless, the precise mechanisms are not completely understood, and this lack of knowledge precluded the development of entirely efficient immunotherapeutic strategies for HPV-associated tumors. As a result, an intense work for attaining how host immune response works, and developing of effective therapies has been applied in the last decade. Based on this, this review aims to discuss the major mechanisms of immune and non-immune cells modulated by hrHPV and the potential and existing immunotherapies involving such mechanisms in HPV-related cancers. It is noticed that the combination of immunotherapies has been demonstrated to be essential for obtaining better results, especially because the possibility of increasing the modulating capacity of the HPV-tumor microenvironment has been shown to be central in strengthening the host immune system.

show abstract

Section: Dendritic Cells and Immune Checkpoint Moleculesmentioning

confidence: 99%

Activities of stromal and immune cells in HPV-related cancers

Júnior

Melo

Barros

et al. 2018

J Exp Clin Cancer Res

View full text Add to dashboard Cite

show abstract

“…A genetically-modified DC vaccine expressing HPV16 E6/E7 fusion oncoproteins was able elicit the action of E6/E7-specific CTLs against cervical carcinoma CaSki cells and induce their apoptosis. These elicited specific protective immunity functions by the HPV-16 E6/E7 fusion protein produced an effective approach against cervical cancer cell growth (65).…”

Section: Preventive and Therapeutic Hpv Vaccinesmentioning

confidence: 99%

Effect of human papillomavirus infection on the immune system and its role in the course of cervical cancer

et al. 2015

View full text Add to dashboard Cite

Abstract. Human papillomavirus (HPV) is widely known as a cause of cervical intraepithelial neoplasia (CIN) and cervical cancer. The mechanisms involved have been studied by numerous studies. The integration of the virus genome into the host cells results in the abnormal regulation of cell cycle control. HPV can also induce immune evasion of the infected cells, which enable the virus to be undetectable for long periods of time. The induction of immunotolerance of the host's immune system by the persistent infection of HPV is one of the most important mechanisms for cervical lesions. The present review elaborates on the roles of several types of immune cells, such as macrophages and natural killer cells, which are classified as innate immune cells, and dendritic cells (DCs), cluster of differentiation (CD)4 + /CD8 + T cells and regulatory T cells, which are classified as adaptive immune cells. HPV infection could effect the differentiation of these immune cells in a unique way, resulting in the host's immune tolerance to the infection. The immune system modifications induced by HPV infection include tumor-associated macrophage differentiation, a compromised cellular immune response, an abnormal imbalance between type 1 T-helper cells (Th1) and Th2 cells, regulatory T cell infiltration, and downregulated DC activation and maturation. To date, numerous types of preventative vaccines have been created to slow down carcinogenesis. Immune response activation-based therapeutic vaccine is becoming more and more attractive for the treatment of HPV-associated diseases.

show abstract

“…Although our study was limited in HCC cell lines, the results supplied us with a theory and practice basis for exploring the antitumor function of CTL immune response in animal models. A report by Wu et al [ 28 ] has shown that DCs loaded with Ad vector carrying human papillomavirus-16 E6/E7 (HPV-16 E6/E7) fusion gene cocultured with isogenous T cells induced CTL response resulting in a remarkably lethal effect on cervical cancer cell CaSki in vitro. Fu et al [ 29 ] also reported the significant inhibition function of CTLs elicited by HPV E6/E7-loaded DCs against tumor-bearing nude mice.…”

Section: Discussionmentioning

confidence: 99%

Construction and Characterization of Adenovirus Vectors Encoding Aspartate-β-Hydroxylase to Preliminary Application in Immunotherapy of Hepatocellular Carcinoma

Zhou

Liu

et al. 2018

Journal of Immunology Research

View full text Add to dashboard Cite

Dendritic cells (DCs) harboring tumor-associated antigen are supposed to be a potential immunotherapy for hepatocellular carcinoma (HCC). Aspartate-β-hydroxylase (AAH), an overexpressed tumor-associated cell surface protein, is considered as a promising biomarker and therapeutic target for HCC. In this study, we constructed adenovirus vector encoding AAH gene by gateway recombinant cloning technology and preliminarily explored the antitumor effects of DC vaccines harboring AAH. Firstly, the total AAH mRNA was extracted from human HCC tissues; the cDNA was amplified by RT-PCR, verified, and sequenced after TA cloning. Gateway technology was used and the obtained 18T-AAH was used as a substrate, to yield the final expression vector Ad-AAH-IRES2-EGFP. Secondly, bone marrow-derived DCs were infected by Ad-AAH-IRES2-EGFP to yield AAH-DC vaccines. Matured DCs were demonstrated by increased expression of CD11c, CD80, and MHC-II costimulatory molecules. A dramatically cell-killing effect of T lymphocytes coculturing with AAH-DCs on HepG2 HCC cell line was demonstrated by CCK-8 and FCM assays in vitro. More importantly, in an animal experiment, the lysis effect of cytotoxic T lymphocytes (CTLs) on HepG2 cells in the AAH-DC group was stronger than that in the control groups. In conclusion, the gateway recombinant cloning technology is a powerful method of constructing adenovirus vector, and the product Ad-AAH-IRES2-EGFP may present as a potential candidate for DC-based immunotherapy of HCC.

show abstract

Cytotoxic T Lymphocytes Elicited by Dendritic Cell-Targeted Delivery of Human Papillomavirus Type-16 E6/E7 Fusion Gene Exert Lethal Effects on CaSki Cells

Cited by 11 publications

References 24 publications

Activities of stromal and immune cells in HPV-related cancers

Activities of stromal and immune cells in HPV-related cancers

Effect of human papillomavirus infection on the immune system and its role in the course of cervical cancer

Construction and Characterization of Adenovirus Vectors Encoding Aspartate-β-Hydroxylase to Preliminary Application in Immunotherapy of Hepatocellular Carcinoma

Contact Info

Product

Resources

About