Cytotoxic Triterpenes From Trametes orientalis

Abstract

One new triterpene, 24-ene-2′-methyl carboxy acetylquercinicate, and 3 known compounds were isolated from the ethyl acetate extract of the fruiting bodies of Trametes orientalis (Yasuda) Imazeki. Their structures were elucidated by means of extensive spectroscopic analysis and compared with data reported in the literature. Furthermore, they were evaluated for their cytotoxicity against 5 human cancer cell lines in vitro. Compound 4 showed significant cytotoxicity, especially to the leukemic HL-60 cell line (IC50 = 1.77 ± 0.07 μM), while compounds 1-3 showed significant cytotoxicity to SMMC-7721 (hepatoma cell line).

Identification of 1,3,4-Thiadiazolyl-Containing Thiazolidine-2,4-dione Derivatives as Novel PTP1B Inhibitors with Antidiabetic Activity

“…After seeding for 24 h, HepG2 cells (2.5 × 10 6 cells/well) were treated with PA (0.4 μM) and MY17 (2–20 μM) for 24 h, followed by treatment with insulin (100 nM) for 10 min. After harvesting, HepG2 cells were lysed with cell lysis buffer, and the protein concentration was measured by BCA protein quantification kit. , Each protein sample was detected by Western blotting using the corresponding primary antibody and secondary antibody. , …”

“…After harvesting, HepG2 cells were lysed with cell lysis buffer, and the protein concentration was measured by BCA protein quantification kit. 43,44 Each protein sample was detected by Western blotting using the corresponding primary antibody and secondary antibody. 45,46 4.7.…”