Cytotoxic triterpenoids and triterpene sugar esters from the medicinal mushroom Fomitopsis betulina

Sofrenić, Ivana; Anđelković, Boban; Todorović, Nina; Stanojković, Tatjana; Vujisić, Ljubodrag; Novaković, Miroslav; Milosavljević, Slobodan; Tešević, Vele

doi:10.1016/j.phytochem.2020.112580

Cited by 21 publications

(22 citation statements)

References 29 publications

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“…The tested compounds were isolated in our previous research. 6 The identification was based on 1D and 2D NMR and HRESIMS spectra and their comparison with the literature data (for NMR data see Supplementary material,…”

Section: Resultsmentioning

confidence: 99%

“…Tables S-I to S-IV). Tested compounds were: pachymic acid (1), 29 polyporenic acid G (2), 6 polyporenic acid F (3), 6 polyporenic acid C (4), 30 piptolinic acid B (5), 8 3-epi-dehydropachymic acid (6), 8 polyporenic acid A (7), 31 fomefficinic acid A (8), 32 12α-hydroxy-3α-(3′-hydroxy-4′-methoxycarbonyl-3′-methylbutyryloxy)-24-methyllanosta-8,24(31)-dien-26-oic acid (9), 31 betulin (10), 32 3α-(acetyloxy)-16α-hydroxy-24-methylene-lanost-8-en-21-oic acid (11) 33 and dehydropachymic acid (12). 34 All the compounds tested exerted a beneficial effect on control lymphocyte cells giving a significant decrease of the frequency of MN in comparison with the control cell cultures (Fig.…”

Section: O N L I N E F I R S Tmentioning

confidence: 99%

“…To the best of our knowledge, in vitro protective effect of lanostanes on chromosomal aberrations was only tested by Mata et al 9 on those isolated from Euphorbia conspicua. Continuing our examination on wild growing F. betulina as a potential source of biologically active compounds, 6 we now report the evaluation of DNA protective activity of the lanostanes 1-12. The cytokinesis-block micronucleus assay (CBMN) used in this study is a standard method for determining the safety of chemicals and pharmaceuticals.…”

Section: Introductionmentioning

confidence: 98%

“…Lanostanes are a group of triterpenoids present in a large number of medicinal fungi such us Ganoderma lucidum, 1 Gloeophyllum odoratum 2 as well as other related fungi, Poria cocos, 3 Laetiporus sulphureus, Inonotus obliquus, Antrodia camphorate, Daedalea dickinsii, Elfvingia applamata, 4 F. pinicola, 5 F. betulina, 6 etc. Many lanostanes have shown great potential as anticancer agents owing to their cytotoxic and apoptotic effects on various cancer cell lines (Meth-A and LLC; 7 K562, 8 HepG2, Huh7 and Hep3B; 4 HL-60; 6 THP-1 7 etc.). To the best of our knowledge, in vitro protective effect of lanostanes on chromosomal aberrations was only tested by Mata et al 9 on those isolated from Euphorbia conspicua.…”

Section: Introductionmentioning

confidence: 99%

“…22 Lanostanes 1-16 were isolated and purified as described. 6 The culture lymphocytes were treated with three concentrations (1, 2, and 4 μg mL -1 ) of tested compounds. One cell culture served as the control and the tested chemical was not added in it.…”

Section: Introductionmentioning

confidence: 99%

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DNA protective activity of triterpenoids isolated from medicinal mushroom Fomitopsis betulina

Sofrenic

Andjelkovic

Vujisić

et al. 2021

JSCS

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Eleven 31-methylenlanostane triterpenoids, i.e. seven 21- and four 26-oic acids, as well as a lupane triterpenoid betulin, isolated from the fruiting bodies of the mushroom Fomitopsis betulina were tested for in vitro protective effect on chromosome aberrations in peripheral human lymphocytes using cytochalasin-B blocked micronucleus (CBMN) assay. Most of the tested compounds exerted a beneficial effect by reducing DNA damage of human lymphocytes more effectively than amifostine, a radioprotective agent, used as a positive control. All the tested compounds decreased MN frequency in concentration dependent manner, with the concentration of 2.0 ?g mL-1 being the most effective - with increase of the concentration the activity slightly decreases. The structure-activity relationship (SAR) studies indicated that the lanostanes containing a conjugated 7,9 (11)-diene system exhibit lower activity than D8-analogues. It was also demonstrated that the DNA protective activities within the D8-lanostane-26-oic acid group are affected by 3-substitution pattern. In the D8 series the oxygenation at C-12 or 16 as well as 21- or 26-oic acid functionality proved beneficial for in vitro protective effect on chromosomal aberrations. Betulin exhibited the lowest protective activity, but still comparable to that of amifostine.

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Section: Resultsmentioning

confidence: 99%