Cytotoxic, Virucidal, and Antiviral Activity of South American Plant and Algae Extracts

Faral-Tello, Paula; Mirazo, Santiago; Dutra, Carmelo; Pérez, Andrés; Geis-Asteggiante, Lucía; Frabasile, Sandra; Koncke, Elina; Davyt, Danilo; Cavallaro, Lorenzo; Heinzen, Horacio; Arbiza, Juan

doi:10.1100/2012/174837

Cited by 40 publications

(20 citation statements)

References 14 publications

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“…Many studies have reported the existence of algae with bioactive compounds that display potent antiviral activity against numerous viruses, such as dengue (Lee et al, 2006;Pujol et al, 2012), avian influenza (Gerber et al, 1958), HIV (Lee et al, 1999;Thuy et al, 2015), human papillomavirus (HPV) (Buck et al, 2006), and picornavirus (Lee et al, 2009). Also, numerous studies have reported algae with antiviral activity against herpes simplex viruses (Faral-Tello et al, 2012;Ribeiro et al, 2012;Hassan et al, 2015). A study performed in Brazil analyzed more than 36 species of algae from its coasts and reported that four of them had significant antiviral activity against both, HSV-1 and HSV-2.…”

Section: Algae-derived Compounds With Antiviral Activity Against Hsvsmentioning

confidence: 99%

Current Antivirals and Novel Botanical Molecules Interfering With Herpes Simplex Virus Infection

et al. 2020

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Herpes simplex viruses type 1 (HSV-1) and type 2 (HSV-2) are highly prevalent within the human population and are characterized by lifelong infections and sporadic recurrences due to latent neuron infection. Upon reactivations, HSVs may manifest either, symptomatically or asymptomatically and be shed onto others through mucosae body fluids. Although, HSVs can produce severe disease in humans, such as lifethreatening encephalitis and blindness, the most common symptoms are skin and mucosal lesions in the oro-facial and the genital areas. Nucleoside analogs with antiviral activity can prevent severe HSV infection, yet they are not very effective for treating skin manifestations produced by these viruses, as they only reduce in a few days at most the duration of lesions. Additionally, HSV variants that are resistant to these antivirals may arise, especially in immunosuppressed individuals. Thus, new antivirals that can reduce the severity and duration of these cutaneous manifestations would certainly be welcome. Here, we review currently available anti-herpetic therapies, novel molecules being assessed in clinical trials and new botanical compounds reported in the last 20 years with antiviral activities against HSVs that might represent future treatments against these viruses.

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Section: Algae-derived Compounds With Antiviral Activity Against Hsvsmentioning

confidence: 99%

Current Antivirals and Novel Botanical Molecules Interfering With Herpes Simplex Virus Infection

et al. 2020

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“…By contrast, Acyclovir demonstrated only minor virucidal effect (IC 50 >200) against HSV-1, as noted by other researchers. 10,26,27 We can assume that the mechanisms of action of thioglucoside 17 are the direct inactivation of virus particles and partially the inhibition of virus attachment and virus binding. It is well known that natural polyphenols are targeted to the HSV-1 glycoproteins, The results are given as mean ± standard deviation (n = 3).…”

Section: Cytotoxicity and Antiviral Activity Of Compoundsmentioning

confidence: 99%

Synthesis and Screening of Anti-HSV-1 Activity of Thioglucoside Derivatives of Natural Polyhydroxy-1,4-Naphthoquinones

Полоник

Крылова²,

Компанец³

et al. 2019

Natural Product Communications

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Four 1,4-naphthoquinone dithioglucoside derivatives based on natural polyhydroxy-1,4-naphthoquinones were synthesized. These thioglucosides were screened for their antiradical and antiviral activity in vitro. Antiradical activity of tested compounds was determined by the 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay. The anti-herpes simplex virus type 1 (anti-HSV-1) activity of thioglucosides was analyzed by the cytopathic effect inhibition assay and mode of antiviral action was determined by the addition of the tested compounds to uninfected cells, to the virus prior to infection, or to herpes-infected cells. Most effective inhibition of HSV-1 replication was observed at pretreatment of virus by the compounds (direct virucidal effect). The dithioglucoside conjugate with the single β-OH group and lipophilic ethyl substituent in naphthoquinone core showed the greatest antiviral activity.

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“…Cercopithecus aethiops (green monkey) VERO cells (ATCC CCL-81 (Faral-Tello et al 2012) were cultured in MEM (PAA E15-888) supplemented with 10 percent FBS (PAA A15-151) and 2 mM L-glutamine at 37ºC and 5percent CO2. To perform the experiments, cells were seeded in complete media in 24-well plates on 12 mm-diameter coverslips.…”

Section: Cell Culturementioning

confidence: 99%

Peer Review #2 of "VERO cells harbor a poly-ADP-ribose belt partnering their epithelial adhesion belt (v0.1)"

2014

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Poly-ADP-ribose (PAR) is a polymer of up to 400 ADP-ribose units synthesized by poly-ADPribose-polymerases (PARPs) and degraded by poly-ADP-ribose-glycohydrolase (PARG). Nuclear PAR modulates chromatin compaction, affecting nuclear functions (gene expression, DNA repair). Diverse defined PARP cytoplasmic allocation patterns contrast with the yet still imprecise PAR distribution and still unclear functions. Based on previous evidence from other models, we hypothesized that PAR could be present in epithelial cells where cadherin-based adherens junctions are linked with the actin cytoskeleton (constituting the adhesion belt). In the present work, we have examined through immunofluorescence and confocal microscopy, the subcellular localization of PAR in an epithelial monkey kidney cell line (VERO). PAR was distinguished colocalizing with actin and vinculin in the epithelial belt, a location that has not been previously reported. Actin filaments disruption with cytochalasin D was paralleled by PAR belt disruption. Conversely, PARP inhibitors 3-aminobenzamide, PJ34 or XAV 939, affected PAR belt synthesis, actin distribution, cell shape and adhesion. Extracellular calcium chelation displayed similar effects. Our results demonstrate the existence of PAR in a novel subcellular localization. An initial interpretation of all the available evidence points towards TNKS-1 as the most probable PAR belt architect, although TNKS-2 involvement cannot be discarded. Forthcoming research will test this hypothesis as well as explore the existence of the PAR belt in other epithelial cells and deepen into its functional implications.

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Cytotoxic, Virucidal, and Antiviral Activity of South American Plant and Algae Extracts

Cited by 40 publications

References 14 publications

Current Antivirals and Novel Botanical Molecules Interfering With Herpes Simplex Virus Infection

Current Antivirals and Novel Botanical Molecules Interfering With Herpes Simplex Virus Infection

Synthesis and Screening of Anti-HSV-1 Activity of Thioglucoside Derivatives of Natural Polyhydroxy-1,4-Naphthoquinones

Peer Review #2 of "VERO cells harbor a poly-ADP-ribose belt partnering their epithelial adhesion belt (v0.1)"

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