Cytotoxicity and anti-biofilm properties of novel hybrid-glass-based caries infiltrant

Nedeljković, Ivana; Doulabi, Behrouz Zandieh; Abdelaziz, Marwa; Feilzer, Albert J.; Exterkate, R.A.M.; Szafert, Sławomir; Gulia, Nurbey; Krejci, Ivo; Kleverlaan, Cornelis J.

doi:10.1016/j.dental.2022.11.018

Cited by 10 publications

(3 citation statements)

References 43 publications

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“…The MTT assay was used for detecting CLDENs’ cytotoxic effects on HIEC-6 cells, L-02 cells, RAW264.7 cells. Based on well-established guidelines ISO 10993.5, the materials for medical use were considered not cytotoxic when the cell viability rate was greater than 70% [ 38 ]. It appeared that the cell viability rate remained above 70% even at the highest concentration (1000 μg/ml) of CLDENs (Fig.…”

Section: Resultsmentioning

confidence: 99%

Novel plant-derived exosome-like nanovesicles from Catharanthus roseus: preparation, characterization, and immunostimulatory effect via TNF-α/NF-κB/PU.1 axis

Ou,

Wang,

Tie

et al. 2023

J Nanobiotechnol

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Background Plant-derived exosomes-like nanovesicles (PDENs) have been found to be advantageous in disease treatment and drug delivery, but research on their biogenesis, compositional analysis, and key marker proteins is still in its infancy, which limits the standardized production of PDENs. Efficient preparation of PDENs continues to be a major challenge. Results Novel PDENs-based chemotherapeutic immune modulators, Catharanthus roseus (L.) Don leaves-derived exosome-like nanovesicles (CLDENs) were isolated from apoplastic fluid. CLDENs were membrane structured vesicles with a particle size of 75.51 ± 10.19 nm and a surface charge of −21.8 mV. CLDENs exhibited excellent stability, tolerating multiple enzymatic digestions, resisting extreme pH environments, and remaining stable in the gastrointestinal simulating fluid. Biodistribution experiments showed that CLDENs could be internalized by immune cells, and targeted at immune organs after intraperitoneal injection. The lipidomic analysis revealed CLDENs’ special lipid composition, which contained 36.5% ether-phospholipids. Differential proteomics supported the origin of CLDENs in multivesicular bodies, and six marker proteins of CLDENs were identified for the first time. 60 ~ 240 μg/ml of CLDENs promoted the polarization and phagocytosis of macrophages as well as lymphocyte proliferation in vitro. Administration of 20 mg/kg and 60 mg/kg of CLDENs alleviated white blood cell reduction and bone marrow cell cycle arrest in immunosuppressive mice induced by cyclophosphamide. CLDENs strongly stimulated the secretion of TNF-α, activated NF-κB signal pathway and increased the expression of the hematopoietic function-related transcription factor PU.1 both in vitro and in vivo. To ensure a steady supply of CLDENs, plant cell culture systems of C. roseus were established to provide CLDENs-like nanovesicles which had similar physical properties and biological activities. Gram-level nanovesicles were successfully obtained from the culture medium, and the yield was three times as high as the original. Conclusions Our research supports the use of CLDENs as a nano-biomaterial with excellent stability and biocompatibility, and for post-chemotherapy immune adjuvant therapy applications. Graphical Abstract

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Section: Resultsmentioning

confidence: 99%

Novel plant-derived exosome-like nanovesicles from Catharanthus roseus: preparation, characterization, and immunostimulatory effect via TNF-α/NF-κB/PU.1 axis

Ou,

Wang,

Tie

et al. 2023

J Nanobiotechnol

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“…The serum-free medium was replaced with the liner extracts. Three wells containing 10Ùª volume of dimethyl sulfoxide (DMSO) served as the positive control [ 24 ]. Three other wells containing cells and culture medium served as the negative control.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of cytotoxicity and antibacterial activity of different pulp capping liners

Moradi,

Abbasi,

Bahador

et al. 2023

Biomaterial Investigations in Dentistry

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This study compares the cytotoxicity and antibacterial activity of five pulp capping liners. This in vitro study was conducted on Fuji II LC glass ionomer, Dycal, Calcimol LC, TheraCal LC, and ACTIVA BioACTIVE. For cytotoxicity, the (MTT) methyl thiazolyl tetrazolium assay was performed on 3 samples from each group of human dental pulp cells (HDPSCs) after 24 h of incubation. The direct contact test (DCT) for antibacterial activity, 6 samples (3 for each material, and 3 for negative control), from each liner were made to evaluate Streptococcus mutans ( S. mutans ), Lactobacillus casei ( L. casei ), and Lactobacillus acidophilus ( L. acidophilus ). Data were analyzed by one-way ANOVA and Tukey’s post-hoc test (alpha = 0.05). Data analysis showed that the cytotoxicity of the materials was significantly different ( p < 0.001). Fuji II LC and ACTIVA BioACTIVE showed strong cytotoxicity, TheraCal LC moderate cytotoxicity, and Dycal and Calcimol LC slight cytotoxicity. The analysis also revealed a significant difference among the materials regarding antibacterial activity ( p < 0.001). Tukey’s test showed that the mean percentage of reduction in colony count was significant for all liners compared with the positive control ( p < 0.001). The mean percentage of reduction in colony count for Dycal was significantly greater than that of Fuji II LC ( p = 0.014), Calcimol LC ( p = 0.003), and TheraCal LC ( p = 0.001). ACTIVA BioACTIVE did not significantly differ from the other materials as regards antibacterial activity. Dycal showed significantly higher antibacterial activity than the other materials.

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“…Frontiers in Bioengineering and Biotechnology frontiersin.org cytotoxicity, CCK-8 experiment was performed using L-929 cells, a common ideal in vitro model for detecting materials' biocompatibility (Yu et al, 2017;Nedeljkovic et al, 2022). Based on the results, the relative cell survival rate decreased with the prolongation of culture time.…”

Section: Figurementioning

confidence: 99%

Bio-inspired nanocomposite coatings on orthodontic archwires with corrosion resistant and antibacterial properties

Chen,

Zheng

et al. 2023

Front. Bioeng. Biotechnol.

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The corrosion resistance and antibacterial properties of fixed orthodontic devices are insufficient in the complex oral cavity, which delays tooth movement and causes enamel demineralization. To overcome the challenges, this research constructs a series of polydopamine-graphene oxide (PDA-GO) nanocoatings on representative NiTi archwires via self-assembly. The morphology, chemical structure, and multifunctional properties of coatings showed tunability dependent on the PDA/GO ratio. Optimized PDA-GO coatings with uniform and dense characteristics prolonged the diffusion path for the corrosive medium and reduced Ni dissolution in NiTi alloys. Meanwhile, the applied coatings endowed NiTi alloys with antibacterial activity against Streptococcus mutans due to the surface structures and inherent properties of PDA-GO. In vitro cytotoxicity tests further verified their good biocompatibility. This bio-inspired nanocomposite coating provides a practical reference for modification of dental metal surfaces to better behave in the intraoral environment.

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Cytotoxicity and anti-biofilm properties of novel hybrid-glass-based caries infiltrant

Cited by 10 publications

References 43 publications

Novel plant-derived exosome-like nanovesicles from Catharanthus roseus: preparation, characterization, and immunostimulatory effect via TNF-α/NF-κB/PU.1 axis

Novel plant-derived exosome-like nanovesicles from Catharanthus roseus: preparation, characterization, and immunostimulatory effect via TNF-α/NF-κB/PU.1 axis

Evaluation of cytotoxicity and antibacterial activity of different pulp capping liners

Bio-inspired nanocomposite coatings on orthodontic archwires with corrosion resistant and antibacterial properties

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