Cytotoxicity and anti-
            <i>Leishmania amazonensis</i>
            activity of
            <i>Citrus sinensis</i>
            leaf extracts

Garcia, Andreza R.; Amaral, Ana Cláudia F.; Azevedo, Mariana M. B.; Cortê-Real, Suzana; Lopes, Rosana Conrado; Alviano, Celuta Sales; Pinheiro, Anderson S.; Vermelho, Alane Beatriz; Rodrigues, Igor A.

doi:10.1080/13880209.2017.1325380

Cited by 30 publications

(14 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The in vitro effect of natural antimicrobials including maltodextrin 15 , citric acid 16 , sodium citrate 17 , silica 18 , malic acid 19 , citrus extract 20 , and olive extract 21 against parasitic infections have been previously described. However, most have a limited impact when administered individually.…”

mentioning

confidence: 99%

The in vitro and in vivo anti-virulent effect of organic acid mixtures against Eimeria tenella and Eimeria bovis

Balta

Marcu

Linton

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Eimeria tenella and Eimeria bovis are complex parasites responsible for the condition of coccidiosis, that invade the animal gastrointestinal intestinal mucosa causing severe diarrhoea, loss of appetite or abortions, with devastating impacts on the farming industry. The negative impacts of these parasitic infections are enhanced by their role in promoting the colonisation of the gut by common foodborne pathogens. The aim of this study was to test the anti-Eimeria efficacy of maltodextrin, sodium chloride, citric acid, sodium citrate, silica, malic acid, citrus extract, and olive extract individually, in vitro and in combination, in vivo. Firstly, in vitro infection models demonstrated that antimicrobials reduced (p < 0.05), both singly and in combination (AG), the ability of E. tenella and E. bovis to infect MDBK and CLEC-213 epithelial cells, and the virulence reduction was similar to that of the anti-coccidial drug Robenidine. Secondly, using an in vivo broiler infection model, we demonstrated that AG reduced (p = 0.001) E. tenella levels in the caeca and excreted faeces, reduced inflammatory oxidative stress, improved the immune response through reduced ROS, increased Mn-SOD and SCFA levels. Levels of IgA and IgM were significantly increased in caecal tissues of broilers that received 0.5% AG and were associated with improved (p < 0.0001) tissue lesion scores. A prophylactic approach increased the anti-parasitic effect in vivo, and results indicated that administration from day 0, 5 and 10 post-hatch reduced tissue lesion scores (p < 0.0001) and parasite excretion levels (p = 0.002). Conclusively, our in vitro and in vivo results demonstrate that the natural antimicrobial mixture (AG) reduced parasitic infections through mechanisms that reduced pathogen virulence and attenuated host inflammatory events.

show abstract

mentioning

confidence: 99%

The in vitro and in vivo anti-virulent effect of organic acid mixtures against Eimeria tenella and Eimeria bovis

Balta

Marcu

Linton

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Macrophage cells (J774A.1 cell line) were cultured in DMEM medium containing 10% FBS at 37°C under 5% CO 2 atmosphere [32]. The macrophages at 10 5 cell/ml concentration were aliquoted in each well of a 96-well plate.…”

Section: Cytotoxicity Assay By Mtt Methodsmentioning

confidence: 99%

The therapeutic effects of larval excretion/secretion of Lucilia sericata on Leishmania major under in vitro and in vivo conditions

Sherafati

Dayer

Ghaffarifar

2021

Preprint

View full text Add to dashboard Cite

Background Leishmaniasis is a neglected infectious disease caused by a kinetoplastid protozoan. The disease generally manifests as characteristic skin lesions. Due to the lack of definitive treatment and drugs without side effects, many studies have focused on natural compounds as promising drugs for its treatment. This study aimed to evaluate the effects of larval excretion/secretion products (ES) of Lucilia sericata in crude and fractionated forms on Leishmania parasites under both in vitro and in vivo conditions. Methods In vitro experiments involved evaluation of ES products on both promastigotes and amastigotes inside infected macrophages, whereas in vivo experiments included comparative treatments of Leishmanial lesions of mice using Eucerin-formulated ES products and glucantime. Results The IC50 values were 38.7 µg/ml, 47.6 µg/ml, 63.3 µg/ml, and 29.1 µg/ml for crude ES, over 10 kDa ES-fraction, under 10 kDa ES-fraction, and glucantime respectively. Significant differences were observed between viability percentages of promastigotes treated with crude ES and its fractions compared to negative control (p < 0.0001). Crude ES was more effective on amastigote than other two ES fractions at 300 µg/ml concentration. Macroscopic measurement of lesion sizes revealed that the reduction of lesion size in mice treated with crude ES followed quicker cascades of healing than in those treated with glucantime and fractionated ES. Conclusion The present study showed that larval ES of Lucilia sericata in both crude and fractionated forms are effective on both intracellular and extracellular forms of L. major. It also provided evidence that the larval ES exerts both topical and systemic therapeutic effects on leishmanial lesions of the model animal.

show abstract

“…Cytotoxicity assay of the C. longa rhizome of different solvents extracts evaluated by the method of (Garcia et al 2017) with some modification. The macrophages RAW264.7 cell lines were used in this study for the cytotoxic assay.…”

Section: H Cytotoxicity Assaymentioning

confidence: 99%

Antileishmanial potential of different extracts of Curcuma longa rhizome against Leishmania donovani

Kumar¹,

Pal²,

Pratap³

et al. 2022

JSR

View full text Add to dashboard Cite

Plants are an excellent source of herbal medicines and their natural products have antimicrobial potential for the management of numerous diseases. This study investigated the antileishmanial activity (IC50 -50% inhibitory activity) of Curcuma longa (rhizome) crude extracts (80% methanol, chloroform, and acetone) against (Leishmania donovani) promastigote and amastigote forms. All three extracts of C. longa exhibited antileishmanial activity on both forms of parasite Leishmania. Miltefosine was used as a reference drug in this study. The methanolic extract showed the maximum antileishmanial activity followed by chloroform and acetone extract against both the forms promastigotes and amastigotes. The cytotoxicity (CC50) of these extracts were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) assay which was ranging from 889.89 to 969.01 µg/ml. The safety parameter was determined in terms of selectivity index (SI) which was found to be highest for methanolic extract (46.71) followed by chloroform (26.38), reference drug miltefosine (11.65) and acetone extract (4.41). Further, GC-MS analysis of 80% methanolic extract was carried out and identified 51 compounds, out of which were secondary metabolite (2,3-dihydrobenzofuran) sesquiterpenes, terpenoids, monoterpene and phenolic acids was most prominent that could be responsible for its antileishmanial activity. The results revealed that all three extracts have potent antileishmanial activity and it could be used in the formulation of herbal medicine against L. donovani in future prospect.

show abstract

Cytotoxicity and anti- Leishmania amazonensis activity of Citrus sinensis leaf extracts

Cited by 30 publications

References 27 publications

The in vitro and in vivo anti-virulent effect of organic acid mixtures against Eimeria tenella and Eimeria bovis

The in vitro and in vivo anti-virulent effect of organic acid mixtures against Eimeria tenella and Eimeria bovis

The therapeutic effects of larval excretion/secretion of Lucilia sericata on Leishmania major under in vitro and in vivo conditions

Antileishmanial potential of different extracts of Curcuma longa rhizome against Leishmania donovani

Contact Info

Product

Resources

About