Cytotoxicity and Antibacterial Activity of Alginate Hydrogel Containing Nitric Oxide Donor and Silver Nanoparticles for Topical Applications

Abstract

Nitric oxide (NO) and silver nanoparticles (AgNPs) are well-known for their antibacterial activity. In this work, S-nitroso-mercaptosuccinic acid (S-nitroso-MSA), a NO donor, and green tea synthesized AgNPs were individually or simultaneously incorporated into alginate hydrogel for topical antibacterial applications. The obtained hydrogels were characterized and the NO release and diffusion of AgNPs and S-nitroso-MSA from alginate hydrogels were investigated. The hydrogels showed a concentration dependent toxicity toward Vero cells. The potent antibacterial effect of the hydrogels was demonstrated toward Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, and Streptococcus mutans UA159. Interestingly, the combination of S-nitroso-MSA and AgNPs into alginate hydrogels had a superior antibacterial effect, compared with hydrogels containing S-nitroso-MSA or AgNPs individually. This is the first report to describe the synthesis, cytotoxicity, and antibacterial effects of alginate hydrogel containing NO donor and AgNPs. These hydrogels might find important local applications in the combat of bacterial infections.

Polypropylene oxide/polyethylene oxide‐cellulose hybrid nanocomposite hydrogels as drug delivery vehicle

“…This end result suggests that the PPO/PEO‐C@Ag hybrid nanocomposite hydrogel would be a better drug carrier and release agent of ornidazole drug in the colon. The present findings are in accordance with previous work 83 . Further, the outcome of these results similar to reported literature where synthesis of Curcumin and silver nanoparticles carried out from polysaccharide‐based hydrogels improved the photodynamic properties of curcumin through metal‐enhanced singlet oxygen effect 84 …”

Antibacterial efficacy from NO-releasing MOF–polymer films

“…Indeed, insufficient delivery is often a factor that results in low, ineffective and/or slow antimicrobial efficacy from many systems and warrants the combination of NO with other agents such as silver nanoparticles. 15,16,41,42 Our previous studies have confirmed that concentrations of NO released from MOF powders can be effective against a wide range of bacteria, including antibiotic resistant strains, such as MRSA. 43 However, to date, our reported studies have focused on the function of and efficacy from MOF powders.…”

H2S- and NO-releasing gasotransmitter platform: A crosstalk signaling pathway in the treatment of acute kidney injury

“…Thus, small RSNOs such as GSNO may transfer the NO group from one thiol to another (S—NO → protein thiols), leading to S-nitrosated proteins, which is a mechanism dependent on the cell’s surrounding chemical environment [ 93 ]. GSNO is an endogenous molecule that increases NO bioavailability and has NO-like activity, such as vasodilation [ 94 ], anti-oxidant effects [ 95 ], tissue repair [ 96 , 97 ], anti-microbial activity [ 98 , 99 ], among others. GSNO (50 μg/kg body weight) has shown renoprotective effects on the treatment of sepsis-induced AKI applied to rat models with lipopolysaccharide-induced sepsis [ 100 ].…”