Cytotoxicity and cellular mechanisms involved in the toxicity of CdS quantum dots in hemocytes and gill cells of the mussel Mytilus galloprovincialis

Katsumiti, Alberto; Gilliland, Douglas; Aróstegui, Inmaculada; Cajaraville, M.P.

doi:10.1016/j.aquatox.2014.02.003

Cited by 140 publications

(69 citation statements)

References 57 publications

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“…[9][10][11] This and earlier studies have demonstrated that following intravenous injection, QD800-RGD was mainly distributed in the liver, spleen, and lung. 27,30,31 Since the kidney is the main excretory organ in vivo, we focused our toxicity assay in the liver, spleen, lung, and kidney.…”

Section: Discussionmentioning

confidence: 92%

“…10,36 QDs-RGD containing 150-200 pmol equivalent of QDs intravenously injected to mice weighing 20-25 g have been shown to be adequate doses for clear in vivo tumor imaging. 27,[30][31][32] In our study, mice weighing 17-20 g were intravenously injected once or twice with QD800-RGD containing 200 pmol equivalent of QD800, which exceeds the maximum dose of QD800-RGD for achieving in vivo tumor imaging.…”

Section: Discussionmentioning

confidence: 99%

“…[3][4][5][6][7][8] Since the core components of QDs are heavy metals (such as Cd, Se, and so on), their toxicity and biosafety have aroused general concern. 9,10 Some studies have shown that due to the release of heavy metals from the QD core and the generation of reactive oxygen free radicals induced by QDs in cells, QDs are toxic to the organism and cells. [9][10][11] However, other studies show that for some functional QDs coated with bioactive substances, no cell or organism toxicity was observed in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

“…9,10 Some studies have shown that due to the release of heavy metals from the QD core and the generation of reactive oxygen free radicals induced by QDs in cells, QDs are toxic to the organism and cells. [9][10][11] However, other studies show that for some functional QDs coated with bioactive substances, no cell or organism toxicity was observed in vitro and in vivo. [12][13][14] Since there is a large number of functionalized QDs species, each having different physical and chemical properties, their toxicity varies greatly.…”

Section: Introductionmentioning

confidence: 99%

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Toxicity assessment of repeated intravenous injections of arginine–glycine–aspartic acid peptide conjugated CdSeTe/ZnS quantum dots in mice

Wang

Yang

Tang

et al. 2014

IJN

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Background Nanotechnology-based near-infrared quantum dots (NIR QDs) have many excellent optical properties, such as high fluorescence intensity, good fluorescence stability, and strong tissue-penetrating ability. Integrin αvβ3 is highly and specifically expressed in tumor angiogenic vessel endothelial cells of almost all carcinomas. Recent studies have shown that NIR QDs linked to peptides containing the arginine–glycine–aspartic acid (RGD) sequence (NIR QDs-RGD) can specifically target integrin αvβ3 expressed in endothelial cells of tumor angiogenic vessels in vivo, and they offer great potential for early cancer diagnosis, in vivo tumor imaging, and tumor individualized therapy. However, the toxicity profile of NIR QDs-RGD has not been reported. This study was conducted to investigate the toxicity of NIR QDs-RGD when intravenously administered to mice singly and repeatedly at the dose required for successful tumor imaging in vivo. Materials and methods A NIR QDs-RGD probe was prepared by linking NIR QDs with the maximum emission wavelength of 800 nm (QD800) to the RGD peptide (QD800-RGD). QD800-RGD was intravenously injected to BALB/C mice once or twice (200 pmol equivalent of QD800 for each injection). Phosphate-buffered saline solution was used as control. Fourteen days postinjection, toxicity tests were performed, including complete blood count (white blood cell, red blood cell, hemoglobin, platelets, lymphocytes, and neutrophils) and serum biochemical analysis (total protein, albumin, albumin/globulin, aspartate aminotransferase, alanine aminotransferase, and blood urea nitrogen). The coefficients of liver, spleen, kidney, and lung weight to body weight were measured, as well as their oxidation and antioxidation indicators, including superoxide dismutase, glutathione, and malondialdehyde. The organs were also examined histopathologically. Results After one or two intravenous injections of QD800-RGD, as compared with control, no significant differences were observed in the complete blood count; biochemical indicators of blood serum, organ coefficient, and oxidation and antioxidation indicators; and no cell necrosis or inflammation were seen in the liver, spleen, kidney, or lung through histopathological examination. Conclusion Our data demonstrate that the single and repeated intravenous injection of QD800-RGD at a dose needed for successful tumor imaging in vivo is not toxic to mice. Our work lays a solid foundation for further biomedical applications of NIR QDs-RGD.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Toxicity assessment of repeated intravenous injections of arginine–glycine–aspartic acid peptide conjugated CdSeTe/ZnS quantum dots in mice

Wang

Yang

Tang

et al. 2014

IJN

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“…1 The cytotoxic effects of Cd-based QDs can be ascribed to the following mechanisms: degradation of the QDs and consequent release of free Cd ions (Cd 2+ ) 2-4 and generation of ROS. [5][6][7] ROS are a group of compounds endowed with high reactivity and short half-lives because of their tendency to give or receive electrons to attain stability. The three major types of ROS are H 2 O 2 , ⋅O 2 − and ⋅OH.…”

Section: Introductionmentioning

confidence: 99%

Dose and time effect of CdTe quantum dots on antioxidant capacities of the liver and kidneys in mice

Wang

Sun

Meng

et al. 2017

IJN

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Although quantum dot (QD)-induced toxicity occurs due to free radicals, generation of oxidative stress mediated by reactive oxygen species (ROS) formation is considered an important mechanism. However, free radical mechanisms are essentially difficult to elucidate at the molecular level because most biologically relevant free radicals are highly reactive and short-lived, making them difficult to directly detect, especially in vivo. Antioxidants play an important role in preventing or, in most cases, limiting the damage caused by ROS. Healthy people and animals possess many endogenous antioxidative substances that scavenge free radicals in vivo to maintain the redox balance and genome integrity. The antioxidant capacity of an organism is highly important but seldom studied. In this study, the dose and time effects of CdTe QDs on the antioxidant capacities of the liver and kidneys were investigated in mice using the electron paramagnetic resonance (EPR) spin-trapping technique. We found that the liver and kidneys of healthy mice contain specific antioxidant capacities that scavenge ·OH and ·O 2 − . Furthermore, oxidative stress markers (superoxide dismutase [SOD], catalase [CAT], glutathione peroxidase [GPx], glutathione [GSH] and malondialdehyde [MDA]) were examined. In dose course studies, the free radical scavenging efficiencies of the liver and kidneys were found to gradually decrease with increasing concentration of CdTe QD exposure. The activities and levels of SOD, CAT, GPx and MDA were observed to increase in treated groups, whereas those of GSH were reduced. The time course studies revealed that the QD-induced antioxidant efficiency reduction was time dependent with GSH decrease and could recover after a period of time. These experimental results offer new information on QD toxicity in vivo. Specifically, CdTe QDs can deplete GSH to reduce the elimination ability of the liver and kidneys for ·OH and ·O 2 − , thus inducing oxidative damage to tissues.

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Effective Transplantation of 2D and 3D Cultured Hepatocyte Spheroids Confirmed by Quantum Dot Imaging

et al. 2018

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Liver transplantation is the most effective treatment for patients with end‐stage liver diseases, but hepatocyte transplantation using spheroids is expected to become an alternative strategy owing to the limited number of liver donors. However, the effects of the transplantation route and spheroid size on cell dynamics and the organ‐specific accumulation of hepatocytes are not well characterized. In this study, the influence of three delivery routes (tail vein, portal vein, and spleen) and three spheroid sizes (50, 100, and 150 µm in diameter) on the accumulation of human fetal hepatocytes labeled with quantum dots in the liver and other organs (heart, lung, kidney, and spleen) of recipient mice is investigated. Ex vivo fluorescence imaging reveals that the accumulation of transplanted cells in the liver is highest for transplantation via the portal vein compared with other routes. The accumulation efficiency depends on spheroid size, and spheroids with a diameter of 50 µm display greater accumulation than that of spheroids of other sizes. It is concluded that hepatocyte transplantation via the portal vein using small spheroids of 50 µm in diameter may be effective for clinical applications.

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Cytotoxicity and cellular mechanisms involved in the toxicity of CdS quantum dots in hemocytes and gill cells of the mussel Mytilus galloprovincialis

Cited by 140 publications

References 57 publications

Toxicity assessment of repeated intravenous injections of arginine–glycine–aspartic acid peptide conjugated CdSeTe/ZnS quantum dots in mice

Toxicity assessment of repeated intravenous injections of arginine–glycine–aspartic acid peptide conjugated CdSeTe/ZnS quantum dots in mice

Dose and time effect of CdTe quantum dots on antioxidant capacities of the liver and kidneys in mice

Effective Transplantation of 2D and 3D Cultured Hepatocyte Spheroids Confirmed by Quantum Dot Imaging

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Cytotoxicity and cellular mechanisms involved in the toxicity of CdS quantum dots in hemocytes and gill cells of the mussel Mytilus galloprovincialis

Cited by 140 publications

References 57 publications

Toxicity assessment of repeated intravenous injections of arginine&ndash;glycine&ndash;aspartic acid peptide conjugated CdSeTe/ZnS quantum dots in mice

Toxicity assessment of repeated intravenous injections of arginine&ndash;glycine&ndash;aspartic acid peptide conjugated CdSeTe/ZnS quantum dots in mice

Dose and time effect of CdTe quantum dots on antioxidant capacities of the liver and kidneys in mice

Effective Transplantation of 2D and 3D Cultured Hepatocyte Spheroids Confirmed by Quantum Dot Imaging

Contact Info

Product

Resources

About

Toxicity assessment of repeated intravenous injections of arginine–glycine–aspartic acid peptide conjugated CdSeTe/ZnS quantum dots in mice

Toxicity assessment of repeated intravenous injections of arginine–glycine–aspartic acid peptide conjugated CdSeTe/ZnS quantum dots in mice