Cytotoxicity and chemical composition of women's personal PM<sub>2.5</sub> exposures from rural China

Lai, Alexandra; Baumgartner, Jill; Schauer, James J.; Rudich, Yinon; Pardo, Michal

doi:10.1039/d1ea00022e

Cited by 6 publications

(2 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies suggest that ROS generation and pro-oxidant cellular states are due to not only bioavailable PAHs but also their specific metabolic pathways (Briedé et al, 2004;Park et al, 2008). To gain insight into the metabolic processes of bioavailable PM-bound organic compounds, we measured the alteration of aryl hydrocarbon receptor activated phase I enzymes as an indicator of chemical surveillance (Lai et al, 2021). (Arrieta et al, 2003).…”

Section: Genomic Instability Intracellular Ros Gene Mutation and Il-8...mentioning

confidence: 99%

In vitro genotoxic and mutagenic potentials of combustion particles from marine fuels with different sulfur contents

Jeong,

Pantzke,

Offer

et al. 2024

Preprint

View full text Add to dashboard Cite

Ship emissions cause serious environmental impacts and adverse effects toward human health. Therefore, the International Maritime Organization (IMO) restricted the fuel sulfur content (FSC) of marine fuels: FSC must be <0.5% m/m or <0.1% m/m in sulfur emission control areas, covering a range of fuels from distillate diesel-like fuels to low-sulfur heavy fuel oils (HFOs). As a result, ship emissions, e.g., sulfur oxides and particulate matter (PM) have been reduced. However, how FSC correlates with the toxicological potential of ship emissions is still uncertain. The objective of this study was to understand how the physical and chemical properties of particulate emissions from a marine engine operating on five marine fuels with different FSCs influence their toxicological outcome. For this scope, cytotoxic, genotoxic, mutagenic, and pro-inflammatory potentials of collected particles were evaluated in lung cell model systems. The involvement of intracellular reactive oxygen species and xenobiotic metabolism was also explored. While PM from different fuels’ combustion resulted in up to approximately 20% of reduction of cytotoxicity at the highest concentration, other toxicological outcomes, including clonogenic and genotoxic potentials, showed a stronger trend with the polycyclic aromatic hydrocarbon contents in PM compared with FSC. This trend was supported by evidence of a significant increase in gene mutation frequency and alterations in cellular mechanisms induced by an aromatic-rich HFO with an intermediate FSC. In conclusion, apart from reducing FSC in marine fuels, additional particle abatement systems should be considered to reduce the adverse effects of particulate emissions from shipping operations on human health.

show abstract

Section: Genomic Instability Intracellular Ros Gene Mutation and Il-8...mentioning

confidence: 99%

In vitro genotoxic and mutagenic potentials of combustion particles from marine fuels with different sulfur contents

Jeong,

Pantzke,

Offer

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…It is worth noting that these vary in cost, physiological relevance, and throughput. Studies have also looked at a variety of environmental pollutants including PM10, PM2.5, and PM0.1 (particulate matter with aerodynamic diameters of less than 10µm, 2.5µm, and 0.1µm, respectively) collected from cities including Beijing, Milan, Seoul and others (21)(22)(23)(24)(25)(26).…”

Section: Introductionmentioning

confidence: 99%

Particulate matter composition drives differential molecular and morphological responses in lung epithelial cells

Engels,

Kamat,

Pafilis

et al. 2023

Preprint

View full text Add to dashboard Cite

Particulate matter (PM) is a ubiquitous component of indoor and outdoor air pollution that is epidemiologically linked to many human pulmonary diseases. PM has many emission sources, making it challenging to understand the biological effects of exposure due to the high variance in chemical composition. However, the effects of compositionally unique particulate matter mixtures on cells have not been analyzed using both biophysical and biomolecular approaches. Here, we show that in a human bronchial epithelial cell model (BEAS-2B), exposure to three chemically distinct PM mixtures drives unique cell viability patterns, transcriptional remodeling, and the emergence of distinct morphological subtypes. Specifically, PM mixtures modulate cell viability and DNA damage responses and induce the remodeling of gene expression associated with cell morphology, extracellular matrix organization and structure, and cellular motility. Profiling cellular responses showed that cell morphologies change in a PM composition-dependent manner. Lastly, we observed that particulate matter mixtures with high contents of heavy metals, such as cadmium and lead, induced larger drops in viability, increased DNA damage, and drove a redistribution among morphological subtypes. Our results demonstrate that quantitative measurement of cellular morphology provides a robust approach to gauge the effects of environmental stressors on biological systems and determine cellular susceptibilities to pollution.

show abstract

A comprehensive review of the production, adoption and sustained use of biomass pellets in Ghana

Mawusi

Shrestha

Chen

et al. 2023

Heliyon

View full text Add to dashboard Cite

Cytotoxicity and chemical composition of women's personal PM_2.5 exposures from rural China

Abstract: Direct measurement with personal samplers captures the chemical complexity of PM2.5 exposures better than fixed monitors. To investigate biological effects, lung cells were exposed to extracts of exposure PM2.5 samples.

Cited by 6 publications

References 73 publications

In vitro genotoxic and mutagenic potentials of combustion particles from marine fuels with different sulfur contents

In vitro genotoxic and mutagenic potentials of combustion particles from marine fuels with different sulfur contents

Particulate matter composition drives differential molecular and morphological responses in lung epithelial cells

A comprehensive review of the production, adoption and sustained use of biomass pellets in Ghana

Contact Info

Product

Resources

About

Cytotoxicity and chemical composition of women's personal PM2.5 exposures from rural China

Abstract: Direct measurement with personal samplers captures the chemical complexity of PM2.5 exposures better than fixed monitors. To investigate biological effects, lung cells were exposed to extracts of exposure PM2.5 samples.

Cited by 6 publications

References 73 publications

In vitro genotoxic and mutagenic potentials of combustion particles from marine fuels with different sulfur contents

In vitro genotoxic and mutagenic potentials of combustion particles from marine fuels with different sulfur contents

Particulate matter composition drives differential molecular and morphological responses in lung epithelial cells

A comprehensive review of the production, adoption and sustained use of biomass pellets in Ghana

Contact Info

Product

Resources

About

Cytotoxicity and chemical composition of women's personal PM_2.5 exposures from rural China