Cytotoxicity and cytokinetic effects of Mitomycin C and/or photochemotherapy in a human colon adenocarcinoma cell line

Ma, L.-W.; Steen, H.B.; Moan, Johan Emelian; Berg, Kristian; Peng, Qian; Sæther, H.; Rimington, C.

doi:10.1016/0020-711x(92)90132-k

Cited by 11 publications

(6 citation statements)

References 15 publications

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“…The potentiation of photodynamic therapy by MMC has previously been described in human colon adenocarcinoma cells using Photofrin as the photosensitizer (Ma et al, 1992a). By studying cell cycle kinetics, it was established that MMC had the effect of causing a greater proportion of cells to accumulate within the Sphase of the cell cycle.…”

Section: Discussionmentioning

confidence: 96%

Effect of photodynamic therapy in combination with mitomycin C on a mitomycin-resistant bladder cancer cell line

Datta

Allman²,

Loh³

et al. 1997

Br J Cancer

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Summary Photodynamic therapy is a method for treating cancer using drugs activated by light. A new compound, 5-aminolaevulinic acid (ALA), is a precursor of the active photosensitizer protoporphyrin IX (PpIX) and has fewer side-effects and much more transient phototoxicity than previous photosensitizers. Cell survival of ALA-mediated photodynamic therapy was measured in the J82 bladder cancer cell line, along with its mitomycin C-resistant counterpart J82/MMC. This demonstrated that mitomycin resistance is not cross-resistant to photodynamic therapy. There was also a suggestion that the mitomycin-resistant cells were more susceptible to photodynamic therapy than the parent cell line. Photodynamic therapy appeared to enhance the effect of mitomycin C, when mitomycin C was given first. This phenomenon was apparent for both drug-resistant and drug-sensitive cell lines. This suggests a possible role for combined mitomycin C and photodynamic therapy in superficial bladder tumours that have recurred despite intravesical cytotoxic drug treatment.

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Section: Discussionmentioning

confidence: 96%

Effect of photodynamic therapy in combination with mitomycin C on a mitomycin-resistant bladder cancer cell line

Datta

Allman²,

Loh³

et al. 1997

Br J Cancer

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“…Mitomycin C has been demonstrated to potentiate photodynamic therapy both at the cellular level (Ma et al, 1992a(Ma et al, , 1993aDatta et al, 1997) and in vivo (Baas et al, 1994(Baas et al, , 1996. This potentiation of PDT is associated with a mitomycin-C induced cell cycle arrest in late S-phase/G2 (Ma et al, 1992b). It would therefore be reasonable to expect that a radiation induced cell cycle arrest in G2 would also enhance the effectiveness of PDT.…”

Section: Discussionmentioning

confidence: 99%

Effect of photodynamic therapy in combination with ionizing radiation on human squamous cell carcinoma cell lines of the head and neck

2000

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Photodynamic therapy (PDT) is a method for the treatment of cancer that involves the administration of systemic or topical photosensitizing drugs that are preferentially taken up by the tumour and then activated in the presence of light to cause tissue destruction (Dougherty, 1988). Photodynamic therapy works by the generation of singlet oxygen that results in damage to cell membrane structures, microvascular ischaemia, and tissue necrosis. Numerous clinical trials of photodynamic therapy have been conducted over the past decade and PDT has been approved for clinical use in recurrent bladder carcinomas, obstructing oesophageal tumours, and early carcinomas of the bladder, oesophagus, stomach, and tracheobronchial tree. PDT has also been shown to provide curative treatment of early carcinomas of the head and neck, including the oral cavity, pharynx, and larynx (Biel, 1995;Feyh, 1996). Initially PDT was tested in advanced cancers of the head and neck that were untreatable or refractory to conventional therapy, however these trials produced only limited success (Schuller et al, 1984;Wile et al, 1984). A recent retrospective review of the clinical data available for the treatment of head and neck neoplasia using photodynamic therapy indicated that complete response rates of 89.5% are achievable for early squamous cell carcinoma of the head and neck (Biel, 1998). Based on a range of photosensitizers and treatment modalities, cure rates of 95% and 80% were obtained for carcinoma in situ and T1 squamous cell carcinoma of the vocal chord and oral cavity/tongue respectively (follow up 70 months) (Biel, 1998).Most of the available photosensitizers, until recently, have been mixtures of porphyrins such as haematoporphyrin derivative and Photofrin (Quadralogic Technologies, Vancouver, Canada). The main problem with these first-generation photosensitizers is that of prolonged skin photosensitivity. Phototoxic incidences of 20-40% have been reported during follow-up of patients having received Photofrin, with a mean duration of skin photosensitivity exceeding 6 weeks (Dougherty et al, 1990).The use of 5-aminolaevulinic acid (ALA) represents a different strategy in the administration of photosensitizers. ALA itself is not the photo-active drug, but rather it induces, in situ, the synthesis of a pure endogenous porphyrin called protoporphyrin IX (PpIX). The formation of PpIX forms part of the haem synthesis pathway and all nucleated cells that use oxidative metabolism are probably capable of forming this photosensitizer. However, malignant tissue appears to preferentially accumulate PpIX, forming the basis of photodynamic therapy in cancer (Battle, 1993;Kennedy and Pottier, 1994). Intravenous ALA is rapidly cleared from the body, with no PpIX fluorescence within the skin or other body organs detectable after 24 h (Kennedy et al, 1991).Because of this reduced phototoxicity and excellent tumour localizing properties, ALA has been used with great success for the treatment of several neoplastic diseases, particularly of the skin, b...

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“…The complementary way in which mitomycin C and PDT target different subcellular organelles suggest how the two treatments might act independently. The enhancement of PDT by mitomycin C was previously described in human colon adenocarcinoma cells, using Photofrin® as the photosensitizer [10]. However, caution is needed when applying results of in vitro PDT to conclusions about treatment at a tissue level, because PDT has a direct cellular effect as well as an indirect effect at a tissue level, through vascular stasis.…”

Section: Discussionmentioning

confidence: 99%

“…mitomycin-C, which target DNA and the cell nucleus. The ability of mitomycin C to enhance the effect of PDT has been established both in vivo [9] and at a cellular level [10,11]. Previous studies have suggested that mitomycin C may also enhance the effect of PDT in a mitomycin C-resistant cell line [12].…”

Section: Introductionmentioning

confidence: 99%

Investigation of sequential mitomycin C and photodynamic therapy in a mitomycin‐resistant bladder cancer cell‐line model

et al. 2003

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with sequential ALA-mediated PDT and mitomycin C, and with the sequence of treatments reversed. Cell survival was estimated using the tetrazolium assay. The relative mitochondrial density of the two cell lines was estimated using flow cytometry to measure 123 rhodamine fluorescence. RESULTSThe effect of sequential mitomycin C followed by ALA-mediated PDT enhanced the effect of PDT in both cell lines. In J82/MMC this effect was marginally supra-additive. When ALAmediated PDT was administered before mitomycin C, the combined effect was 'sub-additive' . 123Rhodamine fluorescence was > 10 times greater in J82/MMC than J82, suggesting a significantly higher mitochondrial density in the former than the latter.

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Cytotoxicity and cytokinetic effects of Mitomycin C and/or photochemotherapy in a human colon adenocarcinoma cell line

Cited by 11 publications

References 15 publications

Effect of photodynamic therapy in combination with mitomycin C on a mitomycin-resistant bladder cancer cell line

Effect of photodynamic therapy in combination with mitomycin C on a mitomycin-resistant bladder cancer cell line

Effect of photodynamic therapy in combination with ionizing radiation on human squamous cell carcinoma cell lines of the head and neck

Investigation of sequential mitomycin C and photodynamic therapy in a mitomycin‐resistant bladder cancer cell‐line model

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