Cytotoxicity and genotoxicity of pulp capping materials in two cell lines

Camargo, Samira Esteves Afonso; Camargo, Carlos Henrique; Hiller, Karl‐Anton; Rode, Sigmar de Mello; Schweikl, Helmut; Schmalz, Gottfried

doi:10.1111/j.1365-2591.2008.01506.x

Cited by 73 publications

(72 citation statements)

References 35 publications

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“…COB or RCP (Ricinus Communis Polyurethane) was originally developed as a biomaterial for bone repair and regeneration after local bone damage. Due to these positive characteristics, the material is considered to be an excellent candidate for use in pulp capping [40].…”

Section: Castor Oil Bean (Cob) Cementmentioning

confidence: 99%

Recent Advances in Pulp Capping Materials: An Overview

Qureshi

Nandakumar

Pratapkumar

et al. 2014

JCDR

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Section: Castor Oil Bean (Cob) Cementmentioning

confidence: 99%

Recent Advances in Pulp Capping Materials: An Overview

Qureshi

Nandakumar

Pratapkumar

et al. 2014

JCDR

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“…Contemporary used methods for this purpose are direct pulp capping and pulpotomy. A final goal of the application of capping materials is to stimulate the dentinogenic potential of the pulp cells [1,2]. The effectiveness of such therapy is mostly dependent on the location and type of injury, the state of tooth development, the capping material applied and the integrity of cavity restoration [3].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of cytotoxicity and pH changes generated by various dental pulp capping materials — an in vitro study

Łuczaj-Cepowicz¹,

Marczuk-Kolada²,

Pawińska³

et al. 2017

Folia Histochem Cytobiol.

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Introduction. Various materials are used in direct dental pulp capping method. Their biocompatibility and alkalizing abilities are of primary importance affecting therapeutic effects. The aim of this study was to evaluate and compare the cytotoxicity of various pulp-capping materials on human gingival fibroblasts and investigate the pH changes induced by these materials. Material and methods. Human gingival fibroblasts were cultured with nine direct pulp materials using culture plate inserts. The cytotoxic effects were recorded by using an MTT-based colorimetric assay after 3 and 24 h. In the second part of the experiment, the materials were inserted in dialysis tubes and transferred into plastic vials containing deionized water. The changes of the medium pH were measured after 3 and 24 h. Results. We showed differences in cell viability of gingival fibroblasts after varied time of exposition for the tested materials. Cell viability after 24 h increased for Dycal, Biopulp, and Calcipro, and decreased for Calcipulpe, Angelus, Angelus White, and ProRoot Regular. Cell viability for ProRoot and Life did not change. Non-setting calcium hydroxide preparations followed by the MTA group and setting calcium hydroxide materials produced the highest pH. All the tested materials significantly increased pH (p < 0.0001) at 24 h. Conclusions. Currently used pulp capping materials varied in their cytotoxicity relative to human gingival fibroblasts and their alkalizing capacities. Since most likely pH does not affect the viability of cultured cells, further investigations are required to determine physicochemical properties of these materials and the biological activity of the dental pulp.

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“…The primary objective of the application materials in vital pulp therapy is to stimulate the dentinogenic capabilities of pulp cells. 1 The success of such therapy is determined by the location and type of injury, the state of tooth development, the kind of capping material used, and the sealing ability of the restoration.…”

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confidence: 99%

“…[4][5][6] Moreover, high pH contributes to the antibacterial action of pulp capping preparations by having a destructive effect on bacterial cell walls and proteins. 1,7 For that reason, the effectiveness of the bioactive action is directly proportional to the ability of hydroxide ions to dissociate.…”

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confidence: 99%