Cytotoxicity and genotoxicity of sewage treatment plant effluents in rainbow trout cells (RTG-2)

Llorente, Marı́a Teresa; Parra, J.; Sánchez-Fortún, S.; Castaño, Argelia

doi:10.1016/j.watres.2012.08.039

Cited by 34 publications

(16 citation statements)

References 28 publications

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“…However, only recently researchers have started to utilize cell cultures in order to evaluate effluents toxicity. There are still a few studies in the literature but some examples are Shi et al (2009) who used HepG2 cell line and MTT assay to evaluate the toxicity of surface water disinfected by different treatments and Llorente et al (2012) who used rainbow trout cells to test the cytotoxicity and genotoxicity of sewage treatment plants effluents.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity assays to evaluate tannery effluents treated by photoelectrooxidation

et al. 2015

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The advanced oxidation process (AOP) is used to increase the treatment efficiency of effluents however, it is necessary to compare the toxicity of treated and untreated effluents to evaluate if the decontamination process does not cause any biological harm. Cultured cells have been previously used to assess the genotoxic and cytotoxic potential of various compounds. Hence, the aim of this work was to assess the applicability of cytotoxicity assays to evaluate the toxicity related to the AOP treatment. Samples of an industrial effluent were collected after their treatment by a conventional method. Cytotoxicity of standard and AOP treated effluents was assessed in CRIB and HEp-2 cell line using the MTT and neutral red assays. We observed decrease at cell viability in the both assays (50% MTT and 13% NRU) when cells were exposed to the AOP treatment in the highest concentration. Thus, cytotoxic assays in cultured cells can be explored as an useful method to evaluate toxicity as well as to optimize effluents treatment process.Keywords: cytotoxicity, cell culture, effluent, photoelectrooxidation, tannery. Ensaios de citotoxicidade na avaliação de efluentes de curtume tratados por fotoeletrooxidação ResumoO processo de oxidação avançada (POA) tem sido usado para aumentar a eficiência do tratamento de efluentes; no entanto, é necessário comparar a toxicidade de efluentes tratados e não tratados para avaliar se o processo de descontaminação não é capaz de causar algum risco biológico. Cultivos celulares têm sido utilizados para avaliar o potencial genotóxico e citotóxico de vários compostos. Assim, o objetivo deste trabalho foi avaliar a aplicabilidade de ensaios de citotoxicidade para avaliar a toxicidade relacionada ao tratamento com POA. As amostras de um efluente industrial foram recolhidas após o tratamento por um método convencional. A citotoxicidade dos efluentes padrão e tratado com POA foi avaliada nas linhagens celulares CRIB e HEp-2 usando os ensaios do MTT e do vermelho neutro. Observou-se diminuição da viabilidade celular em ambos os ensaios (50% MTT e 13% VN) quando as células foram expostas à concentração mais elevada do efluente tratado com POA. Assim, os ensaios de citotoxicidade em cultivos celulares podem ser explorados como um método útil para avaliar a toxicidade, bem como para otimizar os processos de tratamento de efluentes.

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Section: Discussionmentioning

confidence: 99%

Cytotoxicity assays to evaluate tannery effluents treated by photoelectrooxidation

et al. 2015

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“…Daphnia magna is used in aquatic toxicology because of its easy culturing, its high sensitivity to toxins, and its clonal method of reproduction. Other fish assays measure ATP (adenosine triphosphate) as a biochemical indicator of energy stress in white muscle tissue [44][45][46]. In the former, the toxicological effect is death; in the latter, it is the inhibition of normal reproduction [39].…”

Section: Bioassaysmentioning

confidence: 99%

“…These tests are practical but require skilled personnel for culturing and maintaining the organisms. An automated in vitro micronucleus assay developed for the cell line was used to test the genotoxicity, whereas neutral red uptake, kenacid blue protein assay, and ATP content were used to evaluate cytotoxicity [46]. Fish bioassays are very important for environmental monitoring as they are a reflection of the true state of the environment.…”

Section: Bioassaysmentioning

confidence: 99%

Application of Bioassays/Biosensors for the Analysis of Pharmaceuticals in Environmental Samples

Valera

Babington

Broto

et al. 2013

Analysis, Removal, Effects and Risk of Pharmaceuticals in the Water Cycle - Occurrence and Transformation in the Environment

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“…Since chemical analysis is cumbersome and does not warrant exhaustive coverage of all potential toxic substances, biotests have been developed to define the toxic effects of industrial wastewaters at different concentrations and exposure periods (Reifferscheid et al, 2008;Llorente et al, 2012). Metals when in excess into the exposure pathways, cause lethal effects, genotoxicity, mutagenicity, and carcinogenicity to microorganisms, aquatic organisms, plants, animals, and human beings .…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Avena Sativa L. And Trifolium Alexandrinum L. Test System for Detecting the Genotoxic and Phytotoxic Potential of Water Pollution

Khelif¹

2016

Appl Ecol Env Res

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Abstract. The Reghaïa Lake (Algiers, Algeria) is contaminated by the untreated industrial effluents discharge of Reghaïa-Rouiba industrial park, via El Biar River. The present report is the first study which addresses the effects of industrial wastewaters rejected into the lake in light of genotoxic and phytotoxic effects, using Avena sativa L. and Trifolium alexandrinum L. test system. The wastewaters were collected seasonally at the mouth of El Biar River and analyzed using chemical analysis and biological assays, including the germination and root elongation inhibition test, mitotic index and micronucleus test. Results showed that samples collected during the summer season (SM) were high contaminated by heavy metals than that of the winter season (WN). Furthermore, the WN samples have relatively low phytotoxicity on A. sativa seed germination and T. alexandrinum root elongation (no EC50 was determined). Genotoxicity of the SM samples is evident in A. sativa by a significant depression of mitotic activity, induction of several chromosomal aberrations and occurrence of micronuclei in T. alexandrinum. All the results indicated that the samples from the El Biar discharging river in Reghaïa Lake exhibited genotoxicity and argue for the possible use of A. sativa and T. alexandrinum plant system for genotoxicity assays.

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Cytotoxicity and genotoxicity of sewage treatment plant effluents in rainbow trout cells (RTG-2)

Cited by 34 publications

References 28 publications

Cytotoxicity assays to evaluate tannery effluents treated by photoelectrooxidation

Cytotoxicity assays to evaluate tannery effluents treated by photoelectrooxidation

Application of Bioassays/Biosensors for the Analysis of Pharmaceuticals in Environmental Samples

Efficacy of Avena Sativa L. And Trifolium Alexandrinum L. Test System for Detecting the Genotoxic and Phytotoxic Potential of Water Pollution

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