Cytotoxicity and genotoxicity of silver nanoparticles of different sizes in CHO-K1 and CHO-XRS5 cell lines

Souza, Tiago Alves Jorge de; Franchi, Leonardo Pereira; Rosa, Lilian R.; Veiga, Márcia Andréia Mesquita Silva da; Takahashi, Catarina Satie

doi:10.1016/j.mrgentox.2015.11.002

Cited by 102 publications

(51 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study, the comet assay and the cytokinesis‐block micronucleus assay were combined with mouse bone marrow micronuclear experiments to explore the genetic characteristics of two different nanosilver and the dose‐effect relationship of the genetic toxicity of nanosilver from the internal and external perspectives and multiple genetic endpoint. The comet's results suggested that both two kinds of nanosilver could cause DNA damage, which is similar to many studies (Bastos, Duarte, Santos, & Oliveira, ; Souza, Franchi, Rosa, Da Veiga, & Takahashi, ). For example, when PVP‐AgNPs treated cervical cancer cells (HeLa) and breast cancer (MDA‐MB‐231 and MCF‐7) cells for 12 or 24 hours, the results of the comet showed severe DNA damage (Juarez‐Moreno et al, ).…”

Section: Discussionsupporting

confidence: 88%

Genotoxic effects of silver nanoparticles with/without coating in human liver HepG2 cells and in mice

Wang

et al. 2019

J of Applied Toxicology

View full text Add to dashboard Cite

With the rapid expansion of human exposure to silver nanoparticles (AgNPs), the genotoxicity screening is critical to the biosafety evaluation of nanosilver. This study assessed DNA damage and chromosomal aberration in the human hepatoma cell line (HepG2) as well as the effects on the micronucleus of bone marrow in mice induced by 20 nm polyvinylpyrrolidone‐coated nanosilver (PVP‐AgNPs) and 20 nm bare nanosilver (AgNPs). Our results showed that the two types of AgNPs, in doses of 20‐160 μg/mL, could cause genetic toxicological changes on HepG2 cells. The DNA damage degree of HepG2 cells in 20 nm AgNPs was higher than that in 20 nm PVP‐AgNPs, while the 20 nm PVP‐AgNPs caused more serious chromosomal aberration than 20 nm AgNPs. Both kinds of AgNPs caused genetic toxicity in a dose‐dependent manner in HepG2 cells. In the micronucleus test on mouse bone marrow cells, in doses of 10, 50 and 250 mg/kg body weight administered orally for 28 days once a day, the two kinds of AgNPs have no obvious inhibitory effect on the mouse bone marrow cells, and the effect of chromosome aberration could be documented at the high dose of 250 mg/kg. These results suggest that AgNPs have genotoxic effects in HepG2 cells and limited effects on bone marrow in mice; both in vitro and in vivo tests could be of great importance on the evaluation of genotoxicity of nanosilver. These findings can provide useful toxicological information that can help to assess genetic toxicity of nanosilver in vitro and in vivo.

show abstract

Section: Discussionsupporting

confidence: 88%

Genotoxic effects of silver nanoparticles with/without coating in human liver HepG2 cells and in mice

Wang

et al. 2019

J of Applied Toxicology

View full text Add to dashboard Cite

show abstract

“…However, there are still controversies regarding the association between the size and the toxicity of the particles. Some papers have demonstrated that the exposure to greater size particles (100 nm) can cause more adverse biological effects than the smaller ones (10 nm) [44]. Other authors have reported the contrary: a stronger cytotoxicity induced by nanoparticles with smaller dimensions [45,46].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity Evaluation of Ammonia-Modified Graphene Oxide Particles in Lung Cancer Cells and Embryonic Stem Cells

Keremidarska-Markova

Hristova-Panusheva

Andreeva

et al. 2018

Advances in Condensed Matter Physics

View full text Add to dashboard Cite

Potential toxicity of graphene oxide (GO) is a subject of increasing research interest in the recent years. Here, we have evaluated the cytotoxicity of ammonia-modified GO (GO-NH2) and pristine GO particles in human lung cancer cells, A549 and embryonic stem cells, Lep3 exposed to different particles concentrations (0.1, 1, 10, 20, and 50 μg/ml) for different times (24 and 48h). Compared with GO, GO-NH2 particles possessed smaller size, positive surface charge and higher thickness. An increased propensity to aggregation in cell cultures was also found for GO-NH2 particles. Cytotoxicity evaluation revealed that GO-NH2 particles are more toxic than pristine GO. Applied at concentrations of 10, 20 and 50 μg/ml for 24h they affect significantly cell morphology of viable embryonic stem cells whereas human lung cancer A549 cells seem to be relatively more resistant to short-time exposure. After 48h exposure however cell proliferation of A549 cells was strongly suppressed in a dose-dependent manner while the proliferation ability of embryonic stem cells was not affected. These results suggested that both GO particles exert different degree of cytotoxicity which is time, dose and cell dependent. In general, ammonia-modified GO particles are more toxic than the pristine GO which should be taken into account for future biomedical applications.

show abstract

“…The induction of MN by AgNPs has been attributed to size, with MN induction at the small size (20 nm) in both Caco-2 and HepG2 cells but only MN induction in HepG2 cells at a larger size (50 nm) [21,23]. Nevertheless, an opposite effect was reported using the CBMN assay, where the induction of MN increased with the size of the used AgNPs [58]. Although the effects of AgNPs have been attributed to their ability to release silver ions, as demonstrated by the positive induction of MN by silver nitrate [59], negative effects were reported when silver acetate was used as a source of silver ions [22].…”

Section: Fcmn Assay Cbmn Assaymentioning

confidence: 99%

Micronuclei Detection by Flow Cytometry as a High-Throughput Approach for the Genotoxicity Testing of Nanomaterials

et al. 2019

View full text Add to dashboard Cite

Thousands of nanomaterials (NMs)-containing products are currently under development or incorporated in the consumer market, despite our very limited understanding of their genotoxic potential. Taking into account that the toxicity and genotoxicity of NMs strongly depend on their physicochemical characteristics, many variables must be considered in the safety evaluation of each given NM. In this scenario, the challenge is to establish high-throughput methodologies able to generate rapid and robust genotoxicity data that can be used to critically assess and/or predict the biological effects associated with those NMs being under development or already present in the market. In this study, we have evaluated the advantages of using a flow cytometry-based approach testing micronucleus (MNs) induction (FCMN assay). In the frame of the EU NANoREG project, we have tested six different NMs—namely NM100 and NM101 (TiO2NPs), NM110 (ZnONPs), NM212 (CeO2NPs), NM300K (AgNPs) and NM401 (multi-walled carbon nanotubes (MWCNTs)). The obtained results confirm the ability of AgNPs and MWCNTs to induce MN in the human bronchial epithelial BEAS-2B cell line, whereas the other tested NMs retrieved non-significant increases in the MN frequency. Based on the alignment of the results with the data reported in the literature and the performance of the FCMN assay, we strongly recommend this assay as a reference method to systematically evaluate the potential genotoxicity of NMs.

show abstract

Cytotoxicity and genotoxicity of silver nanoparticles of different sizes in CHO-K1 and CHO-XRS5 cell lines

Cited by 102 publications

References 43 publications

Genotoxic effects of silver nanoparticles with/without coating in human liver HepG2 cells and in mice

Genotoxic effects of silver nanoparticles with/without coating in human liver HepG2 cells and in mice

Cytotoxicity Evaluation of Ammonia-Modified Graphene Oxide Particles in Lung Cancer Cells and Embryonic Stem Cells

Micronuclei Detection by Flow Cytometry as a High-Throughput Approach for the Genotoxicity Testing of Nanomaterials

Contact Info

Product

Resources

About