Cytotoxicity and mutagenicity of the fungicides captan and folpet in cultured mammalian cells (CHO/HGPRT system)

O’Neill, J. Patrick; Forbes, Nancy L.; Hsie, Abraham W.

doi:10.1002/em.2860030306

Cited by 18 publications

(7 citation statements)

References 10 publications

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“…Induction of HGPRT-mutants resulted from treatment with 21 of the 33 chemicals tested (Table III), and all of the chemicals that produced a positive response were recognized carcinogens and/or mutagens [Hsie, 1980;O'Neill et al, 1981;Probst et al, 1981;Amacher and Turner, 1982;Soderman, 1982;Neal and Probst, 1983;Oberly et al, 19841. A negative response was obtained with 12 chemicals.…”

Section: Discussionmentioning

confidence: 99%

An evaluation of the cho/hgprt mutation assay involving suspension cultures and soft agar cloning: Results for 33 chemicals

Oberly

Rexroat

Bewsey

et al. 1990

Environ and Mol Mutagen

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The Chinese hamster ovary cell assay (CHO), which measures forward mutation of the HGPRT locus, is used in several laboratories for the detection of mutagens. A procedure involving treatment of CHO cells in suspension culture and mutant selection in soft agar cloning has been developed (Oberly TJ, Bewsey BJ, Probst GS (1987): Mutat Res 182:99-111). In order to evaluate the effectiveness of these modifications, 33 chemicals representing six chemical classes were tested, and the results were compared to findings obtained in other tests for genotoxicity at Lilly Research Laboratories (LRL). A positive response was obtained with 21 chemicals, all of which are recognized mutagens. Of the 12 compounds that produced negative results, 4 were considered to be mutagens and/or carcinogens. Twelve of the compounds mentioned in this report have been previously tested in the CHO/HGPRT assay by other laboratories, and the results showed strong agreement between laboratories. These findings support the conclusion that the use of suspension cultures and soft agar cloning in the CHO assay provides a sensitive test for the identification of mutagens and is a viable alternative to the traditional monolayer procedure of O'Neill et al. (O'Neill JP, Couch DB, Machanoff R, San Sebastian JR, Brimer PA, Hsie AW (1977): Mutat Res 45:103-109).

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Section: Discussionmentioning

confidence: 99%

An evaluation of the cho/hgprt mutation assay involving suspension cultures and soft agar cloning: Results for 33 chemicals

Oberly

Rexroat

Bewsey

et al. 1990

Environ and Mol Mutagen

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“…Folpet and the other dicarboximide compounds have been found to be the pesticides both the most irritant for the skin, the most sensitizing [6,7]. Toxic effects have been reported both in vitro [8][9][10][11] and in vivo [12].…”

Section: Introductionmentioning

confidence: 99%

Quantification methods of folpet degradation products in plasma with HPLC-UV/DAD: Application to an in vivo toxicokinetic study in rats

Canal-Raffin¹,

Receveur²,

Martínez³

et al. 2008

Journal of Chromatography B

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“…The fungicide Captan has been shown to be mutagenic and to induce other genetic effects in procaryotes [Bridges et al, 1973;Bridges, 1975;Shirasu et al, 1976;Siebertet al, 19761, lower eucaryotes [Waters et al, 19801, and mammalian cells [Arlett et al, 1975;Fry and Fiscor, 1978;O'Neill et al, 1981;Garrett et al, 1986;Jingyie and Baoyeng, 19871. In addition, chronic feeding studies of Captan in mice have demonstrated an induction of duodenal hyperplasia and tumor formation [Stauffer Chemical Company, 1985;Chevron Chemical Company, 19801.…”

Section: Introductionmentioning

confidence: 99%

Effects of captan on dna and dna metabolic processes in human diploid fibroblasts

Snyder¹

1992

Environ and Mol Mutagen

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The fungicide Captan has been examined for its effects on DNA and DNA processing in order to better understand the genotoxicity associated with this agent. Captan treatment resulted in production of DNA single strand breaks and DNA-protein cross-links and elicited an excision repair response in human diploid fibroblasts. Captan was also shown to inhibit cellular DNA synthesis and to form stable adducts in herring sperm and human cellular DNA. Misincorporation of nucleotides into Captan-treated synthetic DNA templates was significantly elevated in an in vitro assay using E. coli DNA polymerase I, suggesting that DNA adduct formation by Captan could have mutagenic consequences. In sum, these studies demonstrate that Captan is capable of interacting with DNA at a number of levels and that these interactions could provide the basis for Captan's genotoxicity. The extreme cytotoxicity of this fungicide, however, could be due to other cellular effects since at the IC50 for cell killing, approximately 0.8 microM, none of the above genotoxic events could be detected by the methods employed.

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Cytotoxicity and mutagenicity of the fungicides captan and folpet in cultured mammalian cells (CHO/HGPRT system)

Cited by 18 publications

References 10 publications

An evaluation of the cho/hgprt mutation assay involving suspension cultures and soft agar cloning: Results for 33 chemicals

An evaluation of the cho/hgprt mutation assay involving suspension cultures and soft agar cloning: Results for 33 chemicals

Quantification methods of folpet degradation products in plasma with HPLC-UV/DAD: Application to an in vivo toxicokinetic study in rats

Effects of captan on dna and dna metabolic processes in human diploid fibroblasts

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