Cytotoxicity and Oral Acute Toxicity Studies of b-mangostin Isolated from Cratoxylum arborescens

Abstract

Introduction:The objective of this study was to investigate the cytotoxicity and oral acute toxicity of β-mangostin isolated from Cratoxylum arborescens. Material and methods: Healthy male and female ICR mice (8 weeks) were fed orally with 250 and 500mg/kg of β-mangostin. Body weight of each animal was measured and any gross behavioral change was observed daily. Hematological and clinical biochemical parameters as well as histopathological analysis were carried out on 15 th day. The level of oxidative stress was analyzed using MDA and GSH measurement. Discussion: The results showed that oral administration of the β-mangostin had no adverse effect on the growth rate, hematological and clinical biochemical parameters. Histological studies showed that the treatments did not induce any pathological changes in the liver and kidney. The compound at both the doses did not alter the oxidative stress biomarkers. The in vitro cytotoxicity of β Mangostin was investigated in HepG2, A549, MCF-7, MDA-MB-231 and PC3 cells. There was significant cytotoxicity in both type of breast cancer cells (MCF-7 and MDA-MB-231).In conclusion, our results show that there was no treatment-related acute toxicity in mice following 14-days oral administration of 250 and 500mg/kg of β-mangostin. Conclusion: The results showed that the compound can be selected for detailed in vitro and in vivo breast cancer research.

Synthesis, Spectral Characterisation and Antimicrobial Properties of Cu(II) and Fe(II) Complexes with Xanthone

“…Table 1 represents 1 H-NMR, 13 C-NMR and DEPT of β-mangostin. 1 H-NMR and 13 C-NMR result was compared with reported data by Sen et al [14], Al-Massarani et al, [15] and Syam et al [16] ] appeared as green and dark brown complex respectively. They are stable at room temperature.…”

Synthesis, Characterization and Antimicrobial Properties of Copper(ii) Complex of Heterocyclic Ligands

“…The purity of isolated -mangostin was 98% determined from HPLC analysis. -mangostin 1 H-NMR and 13 C-NMR data were compared with reported data by Shen et al[15]; Al-Massarani et al[16] and Syam et al[17]. The 1 H NMR spectrum showed the presence of one chelated hydroxyl group at  13.66.…”

Local wisdom on processing and utilization of geronggang’s oil for daily remedy and healthcare

“…The results showed that no acute toxicity was identified in experimental rats. The results showed that oral administration of mangostin did not decrease the growth rate, hematology and clinical biochemical parameters and did not alter oxidative stress biomarkers [33]. Histologically, there are no pathological changes in the liver and kidneys [33].…”