Cytotoxicity and Oxidative Stress Evaluation of Alginate/Cockle Shell Powder Nanobiocomposite Bone Scaffold on Osteoblast

Ahmad, Nurnadiah; Bharatham, Hemabarathy; Hamid, Zariyantey Abdul; Zulkipli, Nor Zulaikha

doi:10.17576/jskm-2017-1501-12

Cited by 5 publications

(5 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Comparing these results with those of previous studies (Ahmad et al, 2017;Masson et al, 2014;Sun et al, 2018), HA, chitosan, and carbon nanotube composite biomaterial was not considered cytotoxic. This composite biomaterial appears to provide a favorable environment for use in association with sheep-derived MSCs to assist tissue regeneration.…”

Section: Discussionsupporting

confidence: 82%

“…The authors considered the biomaterials to be non-cytotoxic and emphasized the good quantitative characteristics of this assay. Previously, similar MTT results were obtained for other bone regeneration biomaterials, and no distinction was observed between the negative control and the biomaterial extract samples (Ahmad et al, 2017;Sun et al, 2018).…”

Section: Discussionsupporting

confidence: 77%

See 1 more Smart Citation

Avaliação in vitro do biomaterial compósito à base de hidroxiapatita, quitosana e nanotubo de carbono como adjuvante no reparo ósseo

Paretsis

Gonçalves

Queiroz‐Hazarbassanov

et al. 2021

Braz. J. Vet. Res. Anim. Sci.

View full text Add to dashboard Cite

O compósito à base de hidroxiapatita, quitosana e nanotubo de carbono foi desenvolvido com o intuito de auxiliar na consolidação óssea. Estudos anteriores sugerem que a combinação de substitutos ósseos e células-tronco mesenquimais (CTM) podem auxiliar a potencializar e promover a regeneração óssea. No presente estudo, o biomaterial foi desenvolvido e a viabilidade e a citotoxicidade de células Vero (ATCC CCL-81) e CTM obtidas de medula óssea provenientes de ovinos utilizando ensaios metil-tiazol-tetrazólio, MTT e iodeto de propídeo (PI) foram avaliadas em diferentes concentrações de extrato desse compósito. O compósito demonstrou ausência de citotoxicidade com comportamento semelhante ao grupo controle. Amostras com 50% e 10% de concentração de extrato do compósito mostraram resultados maiores comparados ao grupo controle (ensaio MTT). Esses resultados também sugerem que a presença do biomaterial pode ser utilizada em associação a CTM. Assim, esse estudo conclui que o compósito apresentado de hidroxiapatita, quitosana e nanotubo de cabono não foi considerado citotóxico e pode ser utilizado em teste in vivo.

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 77%

Avaliação in vitro do biomaterial compósito à base de hidroxiapatita, quitosana e nanotubo de carbono como adjuvante no reparo ósseo

Paretsis

Gonçalves

Queiroz‐Hazarbassanov

et al. 2021

Braz. J. Vet. Res. Anim. Sci.

View full text Add to dashboard Cite

show abstract

“…The naturally occurring aragonite from the cockle shells are found to be highly purified and was proved to be safe for human consumption or medical use due to the below detectable levels of heavy metals (Ghafar et al 2017;Hemabarathy et al 2014). The denser nature of aragonite also enhances its capability for drug delivery development and bone grafting making it an attractive biomaterial for tissue engineering applications (Ahmad et al 2017;Saffanah et al 2017;Saidykhan et al 2016).…”

Section: Cocklesmentioning

confidence: 99%

“…Calcium ions are known biomolecules that determines the early cell behavior which are also known as an osteogenic stimulant. Presences of calcium deposits from the cockle shell powder on the surface of the fabricated scaffold (Figure 2(b)) contribute to the topographical factor of the scaffolds that positively affects the cellular response (Ahmad et al 2017;Hemabarathy et al 2014;Peter et al 2010;Sharma et al 2016). Figure 3 shows the favorable adherence of osteoblast around fragments of the nanobiocomposite scaffold in a culture plate as well as on the underlying matrices of the scaffolds.…”

Section: Formulating An Ideal Cockle Shell Powder Based Bone Scaffoldmentioning

confidence: 99%

A Short Review on Cockle Shells as Biomaterials in the Context of Bone Scaffold Fabrication

George

Hamid²,

Perimal

et al. 2019

JSM

Self Cite

View full text Add to dashboard Cite

Cockle shells contribute to a large amount of waste product in South East Asia due to the extensive culturing of the mollusc for consumption. These nacreous materials in the recent years have been gaining wider popularity due to its potential use as biomaterials. As shown in various studies, cockle shell powder consists of 95-98% aragonite form of calcium carbonate (CaCO 3). The calcium carbonate obtained from cockle shells are easily converted into nanoparticles, which have shown encouraging results in bone tissue grafting. With the recent advancement in bone tissue engineering and development of a newer generation of biomaterial based bone scaffolds, the cockle shell powder has promising applications in the near future to be used in the formulation of bone grafting materials. In this review, the use of biomaterials in bone tissue grafting and nacreous materials as potential biomaterials with a focus on the cockle shell and its recent advancement as the main component in the formulation of a nanobiocomposite bone scaffold is discussed.

show abstract

“…Although the development of bone scaffold using SA and cockle shell has been reported [11,19], this research is still in its infancy. The previous works did not comprehensively consider the bioactivity analysis of the scaffold.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Bone Scaffolds from Cockle Shell Waste

2019

View full text Add to dashboard Cite

Bone scaffold is a three-dimensional structure composed of materials that could enhance bone regeneration. Bone scaffolds were prepared using freeze-drying by varying the cockle shell powder concentration where sodium alginate acted as matrix. The scaffolds were then characterized by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, texture analyzer, and liquid displacement method. The bioactivity of the scaffolds was evaluated by immersion into a simulated body fluid solution. Cockle shell powder concentrations affected the bone scaffold characteristics. The increment of the powder concentrations improved the physicochemical properties and bioactivity of the scaffolds.

show abstract

Cytotoxicity and Oxidative Stress Evaluation of Alginate/Cockle Shell Powder Nanobiocomposite Bone Scaffold on Osteoblast

Abstract: Biocompatibility and growth of osteoblast on bone scaffolds play an important role towards their therapeutic application.

Cited by 5 publications

References 9 publications

Avaliação in vitro do biomaterial compósito à base de hidroxiapatita, quitosana e nanotubo de carbono como adjuvante no reparo ósseo

Avaliação in vitro do biomaterial compósito à base de hidroxiapatita, quitosana e nanotubo de carbono como adjuvante no reparo ósseo

A Short Review on Cockle Shells as Biomaterials in the Context of Bone Scaffold Fabrication

Fabrication of Bone Scaffolds from Cockle Shell Waste

Contact Info

Product

Resources

About