Cytotoxicity, antitumor-promoting and antioxidant activities of Annona muricata in vitro

Roduan, Mohd Rohaizad Md; Hamid, Roslida Abd; Cheah, Yoke Kqueen; Mohtarrudin, Norhafizah

doi:10.1016/j.hermed.2018.04.004

Cited by 28 publications

(22 citation statements)

References 47 publications

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“…The phytochemical content found in soursop fruit between alkaloids, Annonaceous acetogenin and phenol, in which if compared with its content in soursop leaves shows that each of the phytochemical classes has a smaller variation in fruit 5 . In addition it has been proven from cytotoxic studies using samples of leaves and soursop fruit with water solvents on Raji cells, showing IC 50 values of 73.1 ± 1.4 μg/mL and 385.2 ± 1.7 μg/mL 16 . Although the content in soursop leaves has proven to be more varied and has the potential as an anticancer, soursop leaves are not a normal part of daily consumption as food.…”

Section: Discussionmentioning

confidence: 98%

Free radical scavenging and cytotoxic assay of soursop fruit juice (Annona muricata Linn.) on cervical cancer cell lines (HeLa)

Widyanto

Putri

Kurniasari

et al. 2020

IJND

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ABSTRAKLatar belakang: Kanker serviks merupakan penyakit yang menduduki posisi kedua penyebab kematian pada wanita. Berbagai terapi pendukung mulai dikembangkan, seperti melalui bahan makanan yang dipercaya memiliki efek anti-kanker. Buah sirsak disebut memiliki kandungan fitokimia seperti Annonaceous acetogenin, flavonoid dan fenol yang bermanfaat sebagai anti-kanker. Tujuan: Penelitian ini dilakukan untuk mengetahui potensi anti-oksidan dan sitotoksisitas dari sari buah sirsak pada sel HeLa secara in vitro. Metode: Penelitian ini dilakukan dengan membuat sari buah sirsak dengan cara diblender kemudian dikeringkan dengan metode freeze-drying, yang kemudian dilanjutkan dengan uji 2,2-diphenyl-1-picrylhydrazyl (DPPH) untuk mengetahui potensi penghambatan radikal bebas, dan potensi sitotoksisitas melalui uji MTT (3-(4,5-dimetiltiazol-2-il)-2,5-difenitetrazolium bromide) assay pada sel kanker serviks HeLa. Hasil: Hasil uji aktivitas anti-oksidan menunjukkan persamaan regresi linier (y=0,0149x – 2,8812) dan nilai perhitungan IC50 sari buah sirsak sebesar 3549 μg/mL dan hasil uji sitotoksisitas menunjukkan persamaan regresi linier (y=0,0197x + 0,3101) dan nilai perhitungan IC50 sari buah sirsak pada sel HeLa sebesar 2522,33 μg/mL.Kesimpulan: Sari buah sirsak memiliki aktivitas anti-oksidan yang sangat rendah dan tidak berpotensi sebagai anti-kanker terhadap sel HeLa secara in vitro. KATA KUNCI : Annona muricata Linn, anti-oksidan, kanker, sitotoksisitas ABSTRACTBackground: Cervical cancer is the second leading cause of death in women. Various supporting therapies have been developed, such as through food ingredients which are believed to have anti-cancer effects. Soursop is known to be high phytochemical content such as Annonaceous acetogenin, flavonoid and phenols which are useful as anti-cancer. Objectives: This research was conducted to determine the antioxidant and cytotoxic activity of soursop juice on HeLa cell lines.Methods: This study started by making the soursop fruit extract by blending then dried with freeze-drying method, and then proceed with 2.2-diphenyl-1-picrylhydrazyl (DPPH) assay to determine the potential free radical scavenging activity, cytotoxic potential activity in vitro through MTT (3-(4,5-dimethylthiazol-2-il)-2,5-difenitetrazolium bromide) assay on HeLa cell lines.Results: The IC50 antioxidant activity of soursop fruit extract is 3549 μg/mL with linear regression equation (y=0.0149x – 2.8812) and the IC50 cytotoxicity test of soursop fruit extracton HeLa was 2522,33 μg/mL with linier regression equation (y=0.0197x + 0.3101). Conclusion: The conclusion in this study is that soursop fruit extract has very low antioxidant activity and has no in vitro potential effect as an anti-cancer on HeLa cell lines. KEYWORDS : Annona muricata Linn, antioxidant, cancer, cytotoxic

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Section: Discussionmentioning

confidence: 98%

Free radical scavenging and cytotoxic assay of soursop fruit juice (Annona muricata Linn.) on cervical cancer cell lines (HeLa)

Widyanto

Putri

Kurniasari

et al. 2020

IJND

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“…In another study by Wang et al [32], annonacin (10 mg/kg) was able to inhibit mouse lung cancer by 57.9% in an in vivo model using hybrid mice (BDF-1) when administered orally. In addition, several studies on the antitumoral effect of Annona muricata leaves extract (AML) were conducted in various in vivo model [33–35]. Recently, the antitumoral effect of Annona muricata was shown to be primarily due to the activity of annonacin [35].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, several studies on the antitumoral effect of Annona muricata leaves extract (AML) were conducted in various in vivo model [33–35]. Recently, the antitumoral effect of Annona muricata was shown to be primarily due to the activity of annonacin [35].…”

Section: Discussionmentioning

confidence: 99%

Modulation of cancer signalling pathway(s) in two -stage mouse skin tumorigenesis by annonacin

Roduan

Hamid

Mohtarrudin

2019

BMC Complement Altern Med

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Background: Annonacin, an annonaceous acetogenin isolated from Annona muricata has been reported to be strongly cytotoxic against various cell lines, in vitro. Nevertheless, its effect against in vivo tumor promoting activity has not been reported yet. Therefore, this study was aimed to investigate antitumor-promoting activity of annonacin via in vivo two-stage mouse skin tumorigenesis model and its molecular pathways involved. Methods: Mice were initiated with single dose of 7,12-dimethylbenz[α]anthracene (DMBA) (390 nmol/100 μL) followed by, in subsequent week, repeated promotion (twice weekly; 22 weeks) with 12-O-tetradecanoylphorbol-13-acetate (TPA) (1.7 nmol/100 μL). Annonacin (85 nM) and curcumin (10 mg/kg; reference) were, respectively, applied topically to DMBA/ TPA-induced mice 30 min before each TPA application for 22 weeks. Upon termination, histopathological examination of skin, liver and kidney as well as genes and proteins expression analysis were conducted to elucidate the potential mechanism of annonacin. Results: With comparison to the carcinogen control, Annonacin significantly increased the tumor latency period and reduced the tumor incidence, tumor burden and tumor volume, respectively. In addition, it also suppressed tumorigenesis manifested by significant reduction of hyperkeratosis, dermal papillae and number of keratin pearls on skin tissues. Annonacin also appeared to be non-toxic to liver and kidney. Significant modulation of both AKT, ERK, mTOR, p38, PTEN and Src genes and proteins were also observed in annonacin-targeted signaling pathway(s) against tumorigenesis. Conclusions: Collectively, results of this study indicate that annonacin is a potential therapeutic compound targeting tumor promoting stage in skin tumorigenesis by modulating multiple gene and protein in cancer signaling pathways without apparent toxicity.

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“…Penelitian secara in vitro melaporkan bahwa A. muricata memiliki efek antimikroba, antiinflamasi, anti protozoa, antioksidan, insektisida, larvisida, dan sitotoksik pada sel tumor. Sedangkan, studi in vivo membuktikan bahwa tanaman ini memiliki aktivitas anxiolitik, anti-stres, anti-inflamasi, kontrasepsi, antitumor, anti-ulkus, penyembuhan luka, hepatoprotektor, anti-ikterik dan hipoglikemik (Coria-Téllez et al, 2018;Gavamukulya et al, 2017 Téllez et al, 2018;Gavamukulya et al, 2017;Roduan et al, 2019) (Liaw et al, 2016;Rady et al, 2018). (Rady et al, 2018;Moghadamtousi et al, 2014;Torres et al, 2012;Deep et al, 2016).…”

Section: Pendahuluanunclassified

EFEK FARMAKOLOGI DAN TOKSIK SIRSAK (Annona muricata): A MINI-REVIEW

Cahyawati

2020

Biomedika

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ABSTRAKAnnona muricata (sirsak) telah digunakan secara tradisional sejak lama untuk mengatasi demam, nyeri, gangguan pernapasan dan kulit, sebagai antiparasit, mengatasi infeksi bakteri, menurunkan tekanan darah, mengatasi peradangan, mengatasi diabetes dan kanker. Oleh karenanya, banyak studi baik in vitro maupun in vivo ditujukan untuk membuktikan manfaat tersebut. Senyawa kimia telah berhasil diisolasi dari tanaman ini diantaranya: alkaloid, fenol, dan asetogenin. Senyawa-senyawa tersebut diyakini berperan terhadap efek farmakologi pada berbagai kondisi penyakit. Walaupun demikian, keamanan atau toksisitas tanaman ini masih dipertanyakan baik untuk pemakaian jangka pendek maupun jangka panjang. Dosis efektif tanaman ini juga belum diketahui secara pasti. Melalui tulisan ini, penulis hendak merangkum berbagai efek farmakologi serta efek toksik yang dapat ditimbulkan oleh Annona muricata berdasarkan hasil penelitian terdahulu.Kata Kunci: Annona Muricata, Sirsak, Efek Farmakologi, Efek ToksikABSTRACTAnnona muricata (sirsak) has been used traditionally for a long time to treated fever, pain, respiratory and skin disorders, as an antiparasitic, to treat bacterial infections, reduce blood pressure, to treat inflammation, diabetes and cancer. Therefore, many studies both in vitro and in vivo were aimed to prove these benefits. Various chemical compounds have been successfully isolated from these plants. These compounds were believed to play a vital role in pharmacological effects in various diseases. Nevertheless, the safety or toxicity of this plant was still questionable for both short and long term use. The effective dose of this plant was also not known with certainty. Through this paper, the author wishes to summarize the various pharmacological and toxic effects that can be caused by Annona muricata based on the results of previous studies.Keywords: Annona Muricata, Soursop, Pharmacological Effects, Toxic Effects

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Cytotoxicity, antitumor-promoting and antioxidant activities of Annona muricata in vitro

Cited by 28 publications

References 47 publications

Free radical scavenging and cytotoxic assay of soursop fruit juice (Annona muricata Linn.) on cervical cancer cell lines (HeLa)

Free radical scavenging and cytotoxic assay of soursop fruit juice (Annona muricata Linn.) on cervical cancer cell lines (HeLa)

Modulation of cancer signalling pathway(s) in two -stage mouse skin tumorigenesis by annonacin

EFEK FARMAKOLOGI DAN TOKSIK SIRSAK (Annona muricata): A MINI-REVIEW

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