Cytotoxicity, Apoptosis Induction and Anti-Metastatic Potential of Oroxylum indicum in Human Breast Cancer Cells

Ragupathi, Naveen Kumar Devanga; George, V. Cijo; Suresh, P.K.; Kumar, R. Ashok

doi:10.7314/apjcp.2012.13.6.2729

Cited by 56 publications

(21 citation statements)

References 28 publications

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“…The measurement of deoxyribonucleic acid (DNA) fragmentation after exposure to chemical substances has been used as a tool for apoptosis detection for a number of years [ 19 ] [ 20 ]. This event which is mediated by nucleases can be measured using DNA content and measuring cells containing less than 1DNA equivalent known as Sub-G 1 .…”

Section: Methodsmentioning

confidence: 99%

Biological Activity of N-Hydroxyethyl-4-aza-2,3-didehydropodophyllotoxin Derivatives upon Colorectal Adenocarcinoma Cells

Vélez

Zayas

Kumar

2014

OJMC

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Etoposide is a chemotherapy drug derived from the natural lignin podophyllotoxin. Our novel generated Aza-podophyllotoxin compounds (AZP 8a & AZP 9a) are analogues of podophyllotoxin and were previously screened for anti-cancer activity through the NCI 60 cell line screening panel showing activity on various cell types including colon cancer. This study expands the toxicological screening by studying apoptosis and various hallmark events as part of the mechanism of action of these compounds on colon cancer cells. The COLO 205 cell line was selected and exposed to AZP to determine the IC50 doses at 24 hours treatment. Apoptosis hallmark events such as migration of phosphatidylserine (PS) to the cell membrane, DNA fragmentation, cell cycle effects, mitochondrial membrane permeabilization and caspase activation were included. Experiments were performed in triplicates for all tested compounds including AZP 8a, AZP 9a, camptothecin as positive control and vehicle as negative control. Our results present contrasting apoptotic activity between the experimental compounds. Compound 8a presented migration of PS (annexin V assay), DNA fragmentation and cell cycle arrest at S phase. Compound 9a presented PS migration with fragmented DNA, cell cycle arrest at S phase, mitochondrial membrane permeabilization and activation of caspase 3, 8 and 9. Compound 8a without the oxygen atoms in ring A appears to cause effects similarly to autophagy as induced by etoposide, a cancer drug analogue of our heterocyclic compounds. Compound 9a with the oxygen atoms in expanded ring A presented induction of cell death following activation of a classical apoptosis pathway. Our results suggest that minor structural differences among these AZP can account for the difference in biological response and cancer cell toxicity.

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Section: Methodsmentioning

confidence: 99%

Biological Activity of N-Hydroxyethyl-4-aza-2,3-didehydropodophyllotoxin Derivatives upon Colorectal Adenocarcinoma Cells

Vélez

Zayas

Kumar

2014

OJMC

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“…For bark or stem bark, many biochemical activities have been assessed like antimicrobial, antidiarrheal, analgesic, cytotoxic, hepatoprotective, gastroprotective, antiproliferative, antimetastatic, antiobesity potential, and antioxidant activities [20][21][22][23][24][25][26][27][28][29][30][31] and seed has antidiabetic potentials and showed synergistic potentials with acarbose [32]. Various flavonoids, namely, 5,7-dihydroxyflavone, 5,7-dihydroxy-3methoxyflavone, 3,5,7-trihydroxyflavone, 5,7,4 -trihydroxy-3-methoxyflavone, 3,5,7,4 -tetrahydroxy flavone, and 5,7,4trihydroxyflavone, were identified and separated from the stem bark portion of O. indicum and structure of isolated chemicals was characterized [33][34][35][36][37][38][39][40].…”

Section: Introductionmentioning

confidence: 99%

Ethnopharmacological Inspections of Organic Extract ofOroxylum indicumin Rat Models: A Promising Natural Gift

Begum

Islam

Begum

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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The stem bark of Oroxylum indicum (O. indicum) was aimed at testing for anti-inflammatory, antiulcerative, antihyperglycemic, and antidyslipidemic activities. Liver enzyme concentration (SGPT, SGOT) had also been assessed. After being extracted in organic solvent, 3 distinct doses, 100, 200, and 400 mg/kg b.w. (p.o.), were used. For edema formation 0.1 ml carrageenan at a dose of 1% w/v was injected into paw of left hind. It showed a fall of edemas 37.50%, 48.34%, and 55.83% while used doses were 100, 200, and 400 mg/kg b.w. (p.o.) individually. The EtOH extract of O. indicum (50%) and its fractions PET, CLF, EtOAc, and nBUT were studied against ethanol-induced gastric mucosal damage. Only PET and n-BuOH exhibited the highest percentage of protection and were 96% and 99%, respectively, persuaded by ethanol. In OGTT glibenclamide revealed reduction of glucose level to 7.55 ± 0.22 mmol/L from 10.57 ± 0.32 mmol/L after 30 minutes. Antihyperglycemic activities were assessed for 8- and 12-week duration in diabetic rats. Glibenclamide reduced glucose level from 33.50±0.31 to 7.90±0.19 mmol/L in 12 weeks. In 12 and 8 weeks, combination therapy lowered blood glucose level to a normal extent by 79% and 61% individually. In antidyslipidemic activities after 12-week treatment, it revealed simvastatin; MEOI (400 mg/kg b.w.) and combination of both reduced TC level by 44%, 28%, and 48% consequently followed by TG and LDL. In 8-week treatment, HDL levels were increased by 34%, 13%, and 36%, and in 12 weeks increased by 36%, 8%, and 38% consequently. Liver enzyme concentration after 12 weeks of treatment with glibenclamide, 400 mg/kg b.w. (p.o.) of MEOI and combination of both, exhibited the fact that concentration of SGPT showed downturn by 43.23%, 8.01%, and 54.86% and SGOT by 42.40%, 5.31%, and 44.85%. This study remarked that O. indicum has anti-inflammatory, antiulcer, antidiabetic, and antidyslipidemic potentials but has no ameliorative effect on liver enzyme.

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“…Also, the polar bark extracts of O. indicum (L.) possess antiproliferative and cytotoxic properties against the human breast cancer cells. Few other studies have also shown that non-polar extract of O. indicum (L.) possesses apoptosis promoting ability due to few bioactive phytochemicals present in the extract [ 18 ]. In the present study, the effect of O. indicum (L.) leaf extract was evaluated against 4-NQO induced oxidative stress in a dose-dependent manner against normal control using albino Wistar rats.…”

Section: Introductionmentioning

confidence: 99%

Alleviation of 4-nitroquinoline 1-oxide induced oxidative stress by Oroxylum indicum (L.) leaf extract in albino Wistar rats

Mohan

Kalaivani

Sundaramoorthy

et al. 2016

BMC Complement Altern Med

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Background4-nitroquinoline 1-oxide (4-NQO) is a mutagen known to be responsible for causing cancer by generating oxidative stress in humans. Oroxylum indicum (L.) possesses various bioactive compounds with antioxidant properties. In this connection, the present study aims to analyze the alleviation of 4-NQO induced oxidative stress in albino Wistar rats using O. indicum (L.) leaf extract.MethodsO. indicum (L.) belonging to the family Bignoniaceae, has anticancer and anti-inflammatory properties. In this study, we observed severe oxidative stress in 4-NQO induced albino Wistar rats when compared to untreated control. Alleviation of this condition was seen after the oral administration of O. indicum (L.) leaf extract at 50, 100, and 200 mg/kg body weight.Results4-NQO (50 ppm) administration in drinking water resulted in the generation of reactive oxygen species (ROS) leading to cellular damage, lipid peroxidation and imbalance in antioxidant status. Administration of O. indicum (L.) leaf extract has alleviated the level of 4-NQO induced oxidative stress by increasing the antioxidant status and decreasing the elevation of liver markers in serum.ConclusionsResults clearly suggest that O. indicum (L.) leaf extract when administered orally in a dose dependent manner has the ability to overcome the oxidative stress induced by 4-NQO with hepatoprotective and lipid protective properties.

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Cytotoxicity, Apoptosis Induction and Anti-Metastatic Potential of Oroxylum indicum in Human Breast Cancer Cells

Cited by 56 publications

References 28 publications

Biological Activity of N-Hydroxyethyl-4-aza-2,3-didehydropodophyllotoxin Derivatives upon Colorectal Adenocarcinoma Cells

Biological Activity of N-Hydroxyethyl-4-aza-2,3-didehydropodophyllotoxin Derivatives upon Colorectal Adenocarcinoma Cells

Ethnopharmacological Inspections of Organic Extract ofOroxylum indicumin Rat Models: A Promising Natural Gift

Alleviation of 4-nitroquinoline 1-oxide induced oxidative stress by Oroxylum indicum (L.) leaf extract in albino Wistar rats

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