Cytotoxicity Assay From Fractions of Hedyotis corymbosa Extract Against Breast Cancer Cell Line T47D

Abstract

Drug discovery for cancer medication is the most important effort that researcher do at this time. Indonesia bio diversities have possibility as a cancer medicine sources. Finding a herbal medicine for cancer treatment is a first step to find a right cancer medicine in the future. This research has already completed for the earlier another research. Some fractions of Hedyotis corymbosa extract has been analyzed using Sulforhodamine B method with UV wavelength 515 nm against T47D cell line, a human breast cancer. There are Hexane extract, Methylene chloride extract and Ethyl acetate extract, and give inhibitory concentration 50 (IC50) of 33.45 µg/ml, 54.59 µg/ml and 52.58 µg/ml, respectively. Ethanolic extract, itself has IC50 of 61.57 µg/ml, whereas IC50 value of Cisplatinum is 9.63 µg/ml. There is a difference between the ethanolic extracts with the other fraction.Keywords: breast cancer, herbal medicine, T47D, Hedyotis corymbosa

Isolation of an Anti-Cancer Asperuloside from Hedyotis corymbosa L.

“…After fractionation, the methylene fraction exhibits an IC 50 of 2.75 µg/mL. Our ethanol extract and its fraction in nhexane, methylene chloride, and ethyl acetate have been previously reported to be inhibitory to breast cancer T47D cell line [13], with IC 50 of 9.63, 33.45, 54.59 and 52.58 µg/mL, respectively.…”

“…Methine carbons at δ C 93.4 and 100.1 expected to have deshielding effect and suggested to be linked with two oxygen atom. By exposure of 13 C NMR, we suggested that it has monoterpene and glucopyranosyl basic skeleton. The basic skeleton of monoterpene was from ten carbon of one methylene sp , and one carbonyl (C=O).…”

“…Inspection of 13 C NMR spectra revealed about 18 signals. Methyl signal that were present at δ H 2.0 (s), was easily determined as carbon at δ C 20.7.…”

Ethanolic Extract of Hedyotis corymbosa L. Inhibits Migration and MMP-9 Activity on Metastatic Breast Cancer Cells

“…This phenomenon occurs due to its major compound, ursolic acid, has been known to exhibit cytotoxic effect after 48 hours of treatment (Harmand, et al, 2005;Li, et al, 2010). Several previous reports also have been revealed that Hedyotis corymbosa L. exhibited cytotoxic effect on cancer cell lines, including K562 cells, WiDr cells, COLO 205 cells, Hep3B cells, H460 cells, also on T47D and MCF-7 breast cancer cells (Pandey, et al, 2012;Meiftasari, et al, 2016;Lee, et al, 2011;Andriyani, et al, 2011;Haryanti, et al, 2009). According to study by Endrini (2011), the cytotoxic effect from Hedyotis corymbosa L. was associated with high antioxidant activities from flavonoid and phenolic compounds in extract.…”

“…extract, Previous study reported that Hedyotis corymbosa L. ethanolic extract showed significant anticancer activity on K562 human leukemia cell lines (Pandey, et al, 2012) and on WiDr colon cancer (Meiftasari, et al, 2016). Moreover, its ethanolic extract also revealed its cytotoxic effect on MCF-7 and T47D breast cancer cells with IC 50 value of 77 µg/mL and 62 µg/mL, respectively (Haryanti, et al, 2009;Andriyani, et al, 2011). However, no study has been done to evaluate the cytotoxic effect of the extract towards metastatic cancer cells.…”