Cytotoxicity Assay Using Brine Shrimp Lethality Test on Collagen-Chitosan Wond Dressing Sterilized by Ultraviolet Light

Abstract

Collagen gives a moist state on the wound area to accelerate the wound healing process. Chitosan is a polymer as known as non-toxic, antibacterial, antifungal, biodegradable, and biocompatible materials. Combination of collagen and chitosan expected to be the best biomaterials as a wound dressing for the healing process. The study aimed to determine cytotoxicity assay on collagen-chitosan wound dressing sterilized by ultraviolet (UV) Light using Brine Shrimp Lethality Test (BSLT) method. The test groups were divided into K0, K1, K2, and K3 groups. K0 contained pure chitosan as a control group, K1 contained collagen 25%-chitosan 75%, K2 contained collagen 50%-chitosan 50%, K3 contained collagen 75%-Chitosan 25%. Collagen extracted from skin and scalp of snakehead fish (Channa striata) then mixed with chitosan until formed collagen-chitosan wound dressing. This study used Brine Shrimp Level Test (BSLT) method with solution concentration 10, 50, 100, 250, 500, 750 and 1000 ppm. Based on the results showed that K0, K1, K2, and K3 group had LC50 > 1000 that proven collagen-chitosan wound dressing was non-toxic materials. As conclusion of the study explained composite wound dressing based on collagen-chitosan in all groups that sterilized under UV-Light along 15 minutes was not toxic based on Brine Shrimp Lethality Test showed LC50> 1000.

Effectivity of uv-light exposure on bacterial and fungal growth in Channa striata collagen-chitosan composite dressing for wound healing

“…UV exposure on composites is able to maintain material sterility. 4 UV spectrum divided into UVA with a wavelength of 400 nm to 315 nm, UVB with 315 nm to 280 nm, and UVC with 280 nm to 200 nm. UVC 32 , UV spectrum entirely could inhibit or inactivation of microorganisms, but based on Martin et al (2008) showed UVC with 265 nm was an optimum wavelength as being the most germicidal effect.…”

“…Wound dressing is categorized into traditional dressing including cotton wool, bandages, plasters, lint's, gauzes, and modern wound dressing including foams, composites, films, hydrogels hydrocolloids. 1,2, 4 Selected biomaterial should be considered as a component wound dressing to ensure wound healing gong faster. Collagen and chitosan are known as excellent biomaterial to produce a various biomedical application.…”

“…6 Collagen from fish is a halal product with some advantages such as Foot and Mouth Disease (FMD), Bovine Spongioform Encelopathy (BSE), and Transmissible Spongioform Encephalopathy (TSE). 4,6,7 Chitosan is a biopolymer that widely uses for wound healing and artificial muscles. It has excellent bio properties such as non-toxic, bacteriostatic, biodegradable, and biocompatible.…”

LC-MS of Grammatophyllum scriptum (Lindl.)Bl. and Cytoxic Activity Test

“…BSLT sering digunakan sebagai uji pendahuluan/ praskrining aktivtas biologis yang sederhana untuk menentukan toksisitas suatu senyawa dari sampel dengan menggunakan hewan uji larva udang (A. salina Leach.). Hasil yang diperoleh dihitung sebagai nilai LC₅₀ (Lethal Concentration), yaitu jumlah konsentrasi ekstrak uji yang dapat menyebabkan kematian larva udang sejumlah 50% setelah masa inkubasi 24 jam (29). Nilai LC₅₀ >1000 ppm dikategorikan tidak beracun (30).…”

study on the properties of zinc-enriched spent brewer’s yeast hydrolysate

“…Судалгаанд авсан цайраар Авгалдай үүсэж буй II-р шатанд 10 авгалдайг авч 5 мл далайн ус агуулсан петрийн аяганд хийсний дараа шинжилж буй дээжнээс 0.5 мл (10-4000 ppm) нэмж, тасалгааны температурт 24 цаг ажиглана. 24 цагийн хугацаанд үхсэн болон амьд үлдсэн авгалдайн тоог тоолж, дараах томьёогоор хоруу чанарыг тодорхойлно[18][19][20]. Гарган авсан цайраар баяжуулсан гидролизатын ерөнхий үзүүлэлтүүд болох уураг, амины азот, тос, чийг, үнс, азот уусалтын индекс зэргийг тодорхойлон, дүнг Хүснэгт 2-д үзүүлэв.…”