Cytotoxicity assessment of functionalized CdSe, CdTe and InP quantum dots in two human cancer cell models

Liu, Jing; Hu, Rui; Liu, Jianwei; Zhang, Butian; Wang, Yucheng; Liu, Xin; Law, Wing Cheung; Liu, Liwei; Ye, Ling; Yong, Ken‐Tye

doi:10.1016/j.msec.2015.07.044

Cited by 95 publications

(36 citation statements)

References 84 publications

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“…This result suggests that the lectin-QD interaction did not affect the biological activity of the lectin, thus allowing its use for the study of the mechanism of action. However, the toxicity of QD remains a concern in biological systems, because of the release of heavy metal ions (as Cd +2 ), the generation of reactive oxygen species, and intracellular effects [65,66]. Nevertheless, the cytotoxicity of QD depends on the fabrication materials, coating and other factors, so their harmfulness cannot be generalized.…”

Section: Discussionmentioning

confidence: 99%

Quantum Dot Labelling of Tepary Bean (Phaseolus acutifolius) Lectins by Microfluidics

et al. 2020

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Lectins are bioactive proteins with the ability to recognize cell membrane carbohydrates in a specific way. Diverse plant lectins have shown diagnostic and therapeutic potential against cancer, and their cytotoxicity against transformed cells is mediated through the induction of apoptosis. Previous works have determined the cytotoxic activity of a Tepary bean (Phaseolus acutifolius) lectin fraction (TBLF) and its anti-tumorigenic effect on colon cancer. In this work, lectins from the TBLF were additionally purified by ionic-exchange chromatography. Two peaks with agglutination activity were obtained: one of them was named TBL-IE2 and showed a single protein band in two-dimensional electrophoresis; this one was thus selected for coupling to quantum dot (QD) nanoparticles by microfluidics (TBL-IE2-QD). The microfluidic method led to low sample usage, and resulted in homogeneous complexes, whose visualization was achieved using multiphoton and transmission electron microscopy. The average particle size (380 nm) and the average zeta potential (−18.51 mV) were determined. The cytotoxicity of the TBL-IE2 and TBL-IE2-QD was assayed on HT-29 colon cancer cells, showing no differences between them (p ≤ 0.05), where the LC50 values were 1.0 × 10−3 and 1.7 × 10−3 mg/mL, respectively. The microfluidic technique allowed control of the coupling between the QD and the protein, substantially improving the labelling process, providing a rapid and efficient method that enabled the traceability of lectins. Future studies will focus on the potential use of the QD-labelled lectin to recognize tumor tissues.

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Section: Discussionmentioning

confidence: 99%

Quantum Dot Labelling of Tepary Bean (Phaseolus acutifolius) Lectins by Microfluidics

et al. 2020

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“…[18] The synthesis of these fluorescent proteins is also tedious and expensive. Fluorescent inorganic nanomaterials, including semiconductor quantum dots [19,20], carbon quantum dots [21][22][23][24], silicon quantum dots [25], upconversion nanoparticles [26] and metal nanoclusters [27], Ln ions doped luminescent nanomaterials [28,29] were perplexed by their toxicity and poor degradability. Therefore, some efforts in searching new methods should be done to deal with such dilemma.…”

Section: Introductionmentioning

confidence: 99%

Facile synthesis of polymeric fluorescent organic nanoparticles based on the self-polymerization of dopamine for biological imaging

Shi

Jiang

Liu

et al. 2017

Materials Science and Engineering: C

151

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Polymeric fluorescent organic nanoparticles (polymer-FONs) have raised considerable research attention for biomedical applications owing to their advantages as compared with fluorescent inorganic nanoparticles and small organic molecules. In this study, we presented an efficient, facile and environment-friendly strategy to produce polymer-FONs, which relied on the self-polymerization of dopamine and polyethyleneimine (PEI) in rather mild conditions. To obtain the final polymer-FONs, aldehyde group-containing copolymers (named as poly(UA-co-PEGMA)) were synthesized by reversible addition-fragmentation chain-transfer polymerization using polyethylene glycol methyl ether methacrylate (PEGMA) and 1-undecen-10-al (UA) as monomers. The dopamine was conjugated onto poly(UA-co-PEGMA) through a multicomponent reaction between UA and dopamine to obtain poly(UA-co-PEGMA)-DA, which was further utilized for preparation of polymer-FONs through self-polymerization of dopamine and PEI. H nuclear magnetic resonance, Fourier transform infrared spectroscopy, transmission electron microscopy and fluorescence spectroscopy were employed to characterize the structure, morphology, compositions and optical properties of these polymer-FONs. Cell viability and cell uptake behavior results suggested that these polymer-FONs possess good biocompatibility and can be potentially utilized for biomedical applications. More importantly, the method can be also applied to fabricate many other multifunctional polymer-FONs with great potential for biomedical applications.

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“…[1][2][3][4][5][6][7][8][9] Traditional fluorescence probes with intense emission have been frequently emerged based on different fluorescent materials such as fluorescent proteins, [10][11][12] organic dyes [13][14][15][16][17] and inorganic quantum dots (IQDs) etc. [18][19][20][21][22][23][24] However, the malicious drawbacks such as low photobleaching and molar absorptivity were encountered in these above mentioned bioprobes based on fluorescent proteins and organic dyes. [25,26] meanwhile IQDs also suffered from deathful defects for their long-time application and accumulation in reticuloendothelial system because of their poor biodegradability and high cytotoxicity.…”

Section: Introductionmentioning

confidence: 99%

Preparation of PEGylated polymeric nanoprobes with aggregation-induced emission feature through the combination of chain transfer free radical polymerization and multicomponent reaction: Self-assembly, characterization and biological imaging applications

Wan

Liu

Mao

et al. 2017

Materials Science and Engineering: C

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Self-assembly of amphiphilic luminescent copolymers is a general route to fabricate fluorescent polymeric microparticles (FPMs). In this work, the FPMs with aggregation-induced emission (AIE) feature were fabricated via the combination of the chain transfer free radical polymerization and "one-pot" multicomponent reaction, which conjugated the aldehyde-containing AIE active dye AIE (CHO-An-CHO) and amino-terminated hydrophilic polymer (ATPPEGMA) using mercaptoacetic acid (MTA) as the "lock" molecule. The structure, chemical compositions, optical properties as well as biological properties of the PPEGMA-An-PPEGMA FPMs were characterized and investigated by means of a series of techniques and experiments in detail. We demonstrated the final copolymers showed amphiphilic properties, strong yellow fluorescence and high water dispersibility. Biological evaluation suggested that PPEGMA-An-PPEGMA FPMs possess low cytotoxicity and can be used for cell imaging. More importantly, many other AIE active FPMs are expected to be fabricated using the similar strategy because of the good substrate and monomer applicability of the multicomponent reaction and chain transfer living radical polymerization. Therefore, we could conclude that the strategy described in this work should be of great interest for fabrication of multifunctional AIE active nanoprobes for biomedical applications.

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Cytotoxicity assessment of functionalized CdSe, CdTe and InP quantum dots in two human cancer cell models

Cited by 95 publications

References 84 publications

Quantum Dot Labelling of Tepary Bean (Phaseolus acutifolius) Lectins by Microfluidics

Quantum Dot Labelling of Tepary Bean (Phaseolus acutifolius) Lectins by Microfluidics

Facile synthesis of polymeric fluorescent organic nanoparticles based on the self-polymerization of dopamine for biological imaging

Preparation of PEGylated polymeric nanoprobes with aggregation-induced emission feature through the combination of chain transfer free radical polymerization and multicomponent reaction: Self-assembly, characterization and biological imaging applications

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