Cytotoxicity characterization of catanionic vesicles in RAW 264.7 murine macrophage-like cells

Kuo, Jung-hua Steven; Jan, Ming‐Shiou; Chang, Chien‐Hsiang; Chiu, Hsin‐Tien; Li, Cih-Ta

doi:10.1016/j.colsurfb.2004.12.008

Cited by 71 publications

(32 citation statements)

References 25 publications

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“…A few recent reports have relevantly addressed the mechanisms behind the toxicity of such vesicles, based on conventional surfactants, towards mammalian cells [36,37]. To our knowledge, the present report is the first to directly address acute toxicity and hemolytic activity/potential ocular irritancy of catanionic vesicles, in particular those synthesized from natural sources (amino acids).…”

Section: Introductionmentioning

confidence: 84%

Physicochemical and toxicological properties of novel amino acid-based amphiphiles and their spontaneously formed catanionic vesicles

Brito

Marques

Silva

et al. 2009

Colloids and Surfaces B: Biointerfaces

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a b s t r a c tThe design of efficient liposomal systems for drug delivery is of considerable biomedical interest. In this context, vesicles prepared from cationic/anionic surfactants may offer several advantages, mainly due to their spontaneity in formation and long-term stability. There is also an impending need to produce less toxic, more biocompatible amphiphiles, while maintaining the desirable aggregation properties. In this work, we present data for acute toxicity to Daphnia magna (IC 50 ), and potential ocular irritation (HC 50 ) for some newly prepared ionic surfactants with dodecyl chains, derived from the amino acids tyrosine (Tyr), serine (Ser), hydroxyproline (Hyp) and lysine (Lys). The micellization behavior of the compounds, evaluated from surface tension measurements, is presented and compared to more conventional ionic amphiphiles. Two types of spontaneouly formed catanionic vesicles, composed either by a dodecyltrimethylammonium bromide (DTAB)/Lys-derivative and or Ser-/Lys-derivative mixture, have also been tested for their ecotoxicity and hemolytic potential. All the micelle-forming surfactants as well as the vesicle-containing mixtures are found to have lower ecotoxicity than the reference surfactant DTAB. Moreover, the results from hemolysis and hemoglobin denaturation tests show that the Tyr-and Lys-derivatives are moderately irritant, whereas the Hyp-and Ser-ones are just slightly irritant. Even more significantly, the vesicle-containing mixtures exhibit lower hemolytic activity than the neat surfactants, a positive result for their potential use in liposomal formulations.

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Section: Introductionmentioning

confidence: 84%

Physicochemical and toxicological properties of novel amino acid-based amphiphiles and their spontaneously formed catanionic vesicles

Brito

Marques

Silva

et al. 2009

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

show abstract

“…The incorporation of DXDAB into liposomes was important to the inhibition of the proliferation of HaCaT and SCC25 cells, and the potentially consequent apoptosis of cells in the G o /G 1 and S-G 2 /M phases. Recently, others have shown that cationic vesicles prepared from neat surfactants induced macrophage-like cell line apoptosis (Aramaki et al, 1999;Kuo et al, 2005). Therefore, the incorporation of DXDAB into phosphatidylcholines vesicles causes obvious cytotoxicity and apoptosis, whose effect may be partially responsible for this cytotoxicity of HaCaT and SCC25 cells.…”

Section: Apoptosis Of Cells In Presence Of Cationic Vesiclesmentioning

confidence: 98%

Effect of chain length on physicochemical properties and cytotoxicity of cationic vesicles composed of phosphatidylcholines and dialkyldimethylammonium bromides

Liang

Chou

2009

Chemistry and Physics of Lipids

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“…Another study has been made on a catanionic vesicle consisting of two oppositely charged surfactants. Kuo et al (2005) characterized the cytotoxicity of catanionic vesicles in RAW 264.7 murine macrophage-like cells. The catanionic vesicles consisted of SDS and hexadecyltrimethylammonium bromide (HTMA/DS), and cholesterol.…”

Section: Discussionmentioning

confidence: 99%

Pharmaceutical applications for catanionic mixtures

Bramer

Dew

Edsman

2007

Journal of Pharmacy and Pharmacology

120

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Mixtures of oppositely charged surfactants, so called catanionic mixtures, are a growing area of research. These mixtures have been shown to form several different types of surfactant aggregates, such as micelles of various forms and sizes, and lamellar structures, such as vesicles. In this review, a short introduction to the field of catanionic mixtures is presented and the pharmaceutical possibilities offered by such mixtures are reviewed. There are several interesting ideas on how to apply catanionic mixtures to improve the delivery of, for example, drug compounds and DNA, or for HIV treatment.

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Cytotoxicity characterization of catanionic vesicles in RAW 264.7 murine macrophage-like cells

Cited by 71 publications

References 25 publications

Physicochemical and toxicological properties of novel amino acid-based amphiphiles and their spontaneously formed catanionic vesicles

Physicochemical and toxicological properties of novel amino acid-based amphiphiles and their spontaneously formed catanionic vesicles

Effect of chain length on physicochemical properties and cytotoxicity of cationic vesicles composed of phosphatidylcholines and dialkyldimethylammonium bromides

Pharmaceutical applications for catanionic mixtures

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