Cytotoxicity effect of honey, bee pollen, and propolis from seven stingless bees in some cancer cell lines

Abstract

Our effort to find new material for anti cancer from natural resources leads us to focus on stingless bee products such as honey, bee pollen, and propolis. The products were from seven stingless bees named Homotrigona fimbriata, Heterotrigona itama, Heterotrigona bakeri, Tetragonula sarawakensis, Tetragonula testaceitarsis, Tetragonula fuscobalteata, Tetragonula laeviceps. The stingless bee products were evaluated for their cytotoxicity effect on MCF-7, HeLa and Caco-2 cancer cell lines. This is the first time to be reported that the honey, ethanol extracts of bee pollen and propolis of H. fimbriata displayed more potent cytotoxicity than other stingless bee products. By chromatography and biological activity-guided fractionation, ethanol extract of propolis from H. fimbriata was fractionated and isolated its active compound named mangiferonic acid. Mangiferonic acid showed a cytotoxicity effect with IC 50 values 96.76 µM in MCF-7, >110.04 µM in HeLa, and > 110.04 µM in Caco-2, respectively. These results exhibited the potential of ethanol extracts from propolis of H. fimbriata to be further developed for drug and experiments to verify the function are essential.

Antineoplastic and Antitrypanosomal Properties of Propolis from Tetragonula biroi Friese

“…Additionally, it is assumed that propolis extracts exhibit cytotoxicity by targeting cell cycle regulators such as cyclin D, cyclin-dependent kinases Cdk-2/4/6, and cyclin-dependent kinase inhibitors, thereby arresting the progression of the cancer cell cycle at the G2/M and G0/G1 phases [ 30 , 31 ]. The anticancer properties exhibited by the isolated compounds 27-hydroxymangiferolic acid and 27-hydroxyisomangiferolic acid are in line with a previous report [ 21 ].…”

“…In the current study, the chemical characterization of propolis resulted in the detection of terpenoids as the major constituents in the propolis sample. The spectral characteristics and the occurrence of terpenoids in the propolis extract obtained here were similar to previous investigations on propolis from Indonesia and the United Kingdom [ 20 , 21 ]. Terpenoids role as antioxidants and promising tools in the treatment of CNS disorders such as Parkinson’s disease has been documented [ 22 ].…”

“…Similarly, in 2017, Nguyen et al confirmed chemical shifts similar to those documented for 27-hydoxyisomangiferonic acid [ 13 ], shown in Figure 4 b. Similar bioactive compounds were obtained in a previous report of a study characterizing propolis from H. fibriata [ 21 ].…”

La miel de abejas sin aguijón: una medicina diferente

“…Después de 48 horas de exposición a la miel observaron una inhibición del crecimiento de las células tumorales; apoyando el papel anticancerígeno de la miel [85]. También, en una línea celular derivada de cáncer de mama [86], cáncer cervical [87], adenocarcinoma cervical [88],…”

Jenis-Jenis Kelulut Dan Tumbuhan Pakannya Pada Peternakan Kelulut Di Desa Koto Baru Kabupaten Kuantan Singingi

“…Popularitas lebah tanpa sengat meningkat pesat, selain dari jumlah propolis yang dihasilkan, budidaya lebah tanpa sengat ini juga dapat memberikan manfaat lainnya bagi manusia maupun alam sekitarnya terutama terhadap tumbuhan yang menjadi bahan pakannya (Priawandiputra et al 2020). Bagi manusia bahkan dapat menjadi pembunuh sel kanker dengan senyawa sitotoxinnya (Arung et al 2021) selain itu juga mengandung senyawa yang dibutuhkan untuk tubuh manusia seperti sukrosa yang sudah sesuai standar SNI (Ridoni, Radam, and Fatriani 2020). Pemahaman jenis lebah madu tanpa sengat dalam usaha budidaya lebah madu sangat penting, salah satunya berkaitan dengan preferensi jenis tumbuhan pakan dan produk yang dihasilkan.…”