Cytotoxicity effect of orthodontic miniscrew-implant in different types of mouthwash

Abstract

CONTEXT: Orthodontic miniscrew implants (OMIs) are widely used as anchorage alternatives, but recent studies revealed the corrosion behavior of OMIs when they come in contact with mouthwashes. The corrosion materials that are released can cause toxicity, allergy, and mutagenicity. AIMS: This study aims to analyze the cytotoxicity effects of OMIs exposed to different types of mouthwash using human gingival fibroblast (HGFs). SETTINGS AND DESIGN: Experimental laboratory research. METHODS AND MATERIAL: Twenty-eight samples of Ti alloy OMIs immersed separately in four groups of different types of mouthwash (chlorhexidine gluconate 0.2% mouthwash (CHX), fluoridated (sodium fluoride 0.2%) mouthwash, chitosan mouthwash 1.5%, and aquadest) for 28 d. Elution of each group and the mouthwash itself were added to the cell culture and incubated for 24 h. Changes in cell viability were performed by MTT Assay. STATISTICAL ANALYSIS USED: Data were tested for normality with Shapiro–Wilk, homogeneity with Levene test, and analyzed using an independent T-test ( P < 0.05). RESULTS: The differences between the cytotoxicity of the elution of MIO and the mouthwash solution itself in the group of CHX and Fluoride were statistically significant ( P < 0.05). No significant differences were found in the group of chitosan and aquadest ( P > 0.05). CONCLUSIONS: The 1.5% chitosan mouthwash can be offered to patients with Ti alloy-based OMIs rather than the 0.2% chlorhexidine gluconate and 0.2% sodium fluoride mouthwashes.

Biocompatibility of a Ti-Rich Medium-Entropy Alloy with Glioblastoma Astrocytoma Cells

“…Many researchers have noted that surfaces with high corrosion resistance improve cell viability [ 34 , 35 , 36 ]. Significantly, the corrosion resistance of β-phase Ti-alloys is generally believed to exceed those of Ti-alloys with other phase structures (α’, α”, α + β, or β) [ 3 , 37 ], due to elements in β-phase alloys that have high corrosion resistance (such as Zr, Nb, Mo, and Ta) [ 38 ].…”

Stability of Mini Implants in the Periodontum during Orthodontic Treatment

“…However, different studies have revealed the corrosion behavior of mini-implants when they come into contact with mouthwashes [31]. Any solid material in a chemical environment results in loss of structural integrity, change of structural characteristics, and loss of material substance [32].…”

The Orthodontic Mini-Implants Failures Based on Patient Outcomes: Systematic Review

“…15 Various studies have suggested reducing inflammation rates by taking daily mouthwash safely. 16,17 According to Nugraha et al, they concluded that Robusta Green Coffee Bean (RGCB) ethanol extract might be effective against periimplantitis bacteria in vitro, and chlorogenic acid in RGCB has antibacterial, anti-inflammatory, antioxidant, pro-osteogenic properties, and antibone resorption. 18 According to some researchers, mini-implant failure is interpreted as the occurrence of implant mobility less than 8 months after insertion, causing the implants to be unable to act as an anchor, and implant replacement is required.…”

“… 15 Various studies have suggested reducing inflammation rates by taking daily mouthwash safely. 16 17 According to Nugraha et al, they concluded that Robusta Green Coffee Bean (RGCB) ethanol extract might be effective against periimplantitis bacteria in vitro , and chlorogenic acid in RGCB has antibacterial, anti-inflammatory, antioxidant, pro-osteogenic properties, and antibone resorption. 18 …”