Cytotoxicity Evaluation of Endodontic Pins on L929 Cell Line

Cannella, Vincenza; Altomare, Roberta; Chiaramonte, Gabriele; Bella, Santina Di; Russotto, Laura; Pisano, Patrizia; Guercio, Annalisa

doi:10.1155/2019/3469525

Cited by 96 publications

(56 citation statements)

References 14 publications

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“…According to the UNI EN ISO 10993 regulation, the L929 cell line was chosen for cytotoxicity assay, cell proliferation test, and cell morphology evaluation by haematoxylin/eosin staining. As demonstrated in a previous study, L929 cells are suitable to undergo a cytotoxicity test by MTS assay, as they are sensible to the reference materials indicated in the rule and able to well respond against any cytotoxic substance released in the culture media [12]. The…”

Section: Discussionmentioning

confidence: 91%

“…All plates were incubated at +37 ± 1°C in a 5% CO 2 atmosphere and examined microscopically after 24 h, 48 h, and 72 h of incubation in order to assess vitality and general morphology of cells. The vitality MTS assay was performed at the same time points as previously described [12]. The absorbance recorded is directly proportional to the number of living cells.…”

Section: Sample Preparationmentioning

confidence: 99%

“…The interaction of cell cultures with potential toxic compounds released by a biomaterial induces a detectable biological response through which it is possible to establish the safety of a material under examination. Cytotoxicity tests allow to highlight the possible alterations in basic cellular functions through the analysis of cellular metabolism, morphology, and the proliferation rate or vitality [12]. The UNI EN ISO 10993/2009 rule, part five, describes suitable in vitro methods to perform the biological evaluation of medical devices [13].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Biocompatibility Evaluation of Nine Dermal Fillers on L929 Cell Line

Cannella

Altomare

Leonardi

et al. 2020

BioMed Research International

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Objective. Biomaterial research for soft tissue augmentation is an increasing topic in aesthetic medicine. Hyaluronic acid (HA) fillers are widely used for their low invasiveness and easy application to correct aesthetic defects or traumatic injuries. Some complications as acute or chronic inflammation can occur in patients following the injection. Biocompatibility assays are required for medical devices intended for human use, in order to prevent damages or injuries in the host. In this study, nine HA fillers were tested in order to evaluate their cytotoxicity and their effects on L929 cell line, according to the UNI EN ISO 10993 regulation. Methods. Extracts were prepared from nine HA fillers, and MTS viability assay was performed after 24 h, 48 h, and 72 h of exposure of cells to extracts. Cells cultured with HA filler extracts were monitored for up to 72 h, counted, and stained with haematoxylin/eosin in order to evaluate the cell proliferation rate and morphology. Results. None of the filler tested showed a cytotoxic effect. Two samples showed a higher vitality percentage and higher cell number while two samples showed a lower vitality percentage and lower cell number at 72 h. Conclusion. Data obtained suggest that although examined fillers are not cytotoxic, they show different effects on the in vitro cell proliferation rate. In vitro studies of medical devices could lead to important implications since these could aid to predict effects about their in vivo application. These easy and rapid assays could be useful to test new materials intended for human use avoiding animal tests.

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Section: Discussionmentioning

confidence: 91%

Section: Sample Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In Vitro Biocompatibility Evaluation of Nine Dermal Fillers on L929 Cell Line

Cannella

Altomare

Leonardi

et al. 2020

BioMed Research International

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“…In order to evaluate the cytotoxicity of composite membranes, extracts of the material and MTT (3-dimethylthiazol-2,5-diphenyltetrazolium bromide; Aldrich 135038, Sigma-Aldrich, Saint Louis, USA) assay were applied using L929 mouse fibroblasts as stated in the International Organization for Standardization 109993-5 [23,24]. Briefly, the extracts were prepared with sterile membrane samples incubated in the cell-cultured medium (1 g membrane in 5 mL of medium) at 37 • C under 5% CO 2 atmosphere and extracted after 1, 7, 14, and 21 days.…”

Section: Mtt Assaymentioning

confidence: 99%

“…This test was performed according to the International Organization for Standardization (ISO) norms [24], using the indirect contact method with extracts as applied to L929 cells, a line derived from mouse fibroblasts [23]. A MTT cell viability assay was performed to evaluate the effect of the C 3 S addition on the cellular performance of the PLLA/C 3 S composite samples in terms of cellular viability.…”

Section: Mtt Assaymentioning

confidence: 99%

Fabrication of PLLA/C3S Composite Membrane for the Prevention of Bone Cement Leakage

et al. 2019

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Kyphoplasty is an important treatment for stabilizing spine fractures due to osteoporosis. However, leakage of polymethyl-methacrylate (PMMA) bone cement during this procedure into the spinal canal has been reported to cause many adverse effects. In this study, we prepared an implantable membrane to serve as a barrier that avoids PMMA cement leakage during kyphoplasty procedures through a hybrid composite made of poly-l-lactic acid (PLLA) and tricalcium silicate (C 3 S), with the addition of C 3 S into PLLA matrix, showing enhanced mechanical and anti-degradation properties while keeping good cytocompatibility when compared to PLLA alone and most importantly, when this material design was applied under standardized PMMA cement injection conditions, no posterior wall leakage was observed after the kyphoplasty procedure in pig lumbar vertebral bone models. Testing results assess its effectiveness for clinical practice.2 of 17 the created cavity after balloon inflation during the kyphoplasty procedure [10]. Following which the balloon is re-inserted and inflated, creating a cement shell or a cement membrane around the inner walls of the created cavity, and another batch of cement was mixed and injected into the remaining cavity thereafter with limited cement leakage possibility from initial cement setting. Although this double cement application with PMMA as the cement anti-leakage shell or membrane has demonstrated its efficacy in clinical trial during the kyphoplasty procedure [11], the long term complications might be devastating due to PMMA presence on the bone cement surface [9], which makes the development of a new material design for anti-leakage membrane necessary. For example, Tetsushi Taguchi et al. had fabricated the reactive poly(vinyl alcohol) (PVA) membrane for the prevention of bone cement leakage with good potential for implantable balloon kyphoplasty, but further investigations on its clinical efficiency are still in progress [12].On the other hand, polymeric membranes fabricated from a single material more often have limited biological performance compared to the use of hybrid biomaterials composed of biodegradable synthetic polymers and inorganic materials, with the hybrid biomaterials fitting better to bone tissue engineering applications due to their similar compositions to natural bones [13,14]. For example, biodegradable polymers such as poly(L-lactic acid) (PLLA) (L-lactic acid isomer of polylactic acid (PLA), an aliphatic thermoplastic polyester obtained by polymerizing lactide monomers) have been used for scaffold design doped with dicalcium silicate (C 2 S) nanoparticles as an ideal candidate for novel bone graft substitutes with enhanced mechanical and biodegradable properties, and biointeractive nature [15,16]. Scaffolds fabricated from (PLLA)/dicalcium phosphate dihydrate (DCPD) composite by indirect casting had also shown to effectively improve the mechanical strength and biocompatibility for the repair of bone defects when compared to the scaffold from neat PLLA [17]. From which the h...

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Enhancement of functional properties by blending cocoon extracted Antheraea mylitta silk fibroin with polyvinyl alcohol for applications in biomedical field

Batra

Bansal

Yadav

et al. 2021

J of Applied Polymer Sci

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In this study, regenerated Antheraea mylitta silk fibroin (ASF) and polyvinyl alcohol (PVA) dissolved in formic acid were blended together in variable ratios to prepare blend films. The blend films presented homogeneous morphology with no visible phase separation as observed in scanning electron microscope (SEM). The structural analysis of blend films through fourier transform infrared spectroscopy and X-ray diffraction indicated the formation of co-crystals due to interaction of fibroin with PVA molecular chains. The thermal properties assessed using thermogravimetric analyzer and differential scanning calorimeter supported the molecular interactions with altered thermal stability of blend films. Incorporation of PVA into ASF enhanced the mechanical properties of blend films. The water uptake capacity, biocompatibility and biodegradability were analyzed. The L929 fibroblast skin cells seeded on blend films were found to have attached the film surface and started attaining their conventional morphology after 48 h, as observed under SEM. Alamar blue assay confirmed the cytocompatibility of blend films with more than 95% cells being viable. Based on the aforementioned encouraging results, we can assert the utility of these blend films in biomedical field for tissue regeneration applications.

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Cytotoxicity Evaluation of Endodontic Pins on L929 Cell Line

Cited by 96 publications

References 14 publications

In Vitro Biocompatibility Evaluation of Nine Dermal Fillers on L929 Cell Line

In Vitro Biocompatibility Evaluation of Nine Dermal Fillers on L929 Cell Line

Fabrication of PLLA/C3S Composite Membrane for the Prevention of Bone Cement Leakage

Enhancement of functional properties by blending cocoon extracted Antheraea mylitta silk fibroin with polyvinyl alcohol for applications in biomedical field

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